The influence of unsaturation on the phase transition temperatures of a series of heteroacid phosphatidylcholines containing twenty-carbon chains

Keough, K. M. W.; Giffin, Bruce F.; Kariel, N.

doi:10.1016/0005-2736(87)90129-5

Cited by 62 publications

(18 citation statements)

References 29 publications

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“…Especially, they have an unsaturated chain in either sn -1 or sn -2 position. A few reports have been made on phase transitions of PCs with mixed acyl chains of a saturated and an unsaturated one [48–50]. We selected six kinds of mixed-chain PCs with an unsaturated acyl chain in the sn -1 or sn -2 position, 1-oleoyl-2-stearoyl-PC (OSPC), 1-oleoyl-2-palmitoyl-PC (OPPC), 1-oleoyl-2-myristoyl-PC (OMPC) and 1-stearoyl-2-oleoyl-PC (SOPC), 1-palmitoyl-2-oleoyl-PC (POPC), 1-myristoyl-2-oleoyl-PC (MOPC).…”

Section: Effect Of Acyl Chain Structure On the Phase Behavior Of Pmentioning

confidence: 99%

Thermotropic and Barotropic Phase Behavior of Phosphatidylcholine Bilayers

Matsuki

Goto

Tada

et al. 2013

IJMS

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Bilayers formed by phospholipids are frequently used as model biological membranes in various life science studies. A characteristic feature of phospholipid bilayers is to undergo a structural change called a phase transition in response to environmental changes of their surroundings. In this review, we focus our attention on phase transitions of some major phospholipids contained in biological membranes, phosphatidylcholines (PCs), depending on temperature and pressure. Bilayers of dipalmitoylphosphatidylcholine (DPPC), which is the most representative lipid in model membrane studies, will first be explained. Then, the bilayer phase behavior of various kinds of PCs with different molecular structures is revealed from the temperature–pressure phase diagrams, and the difference in phase stability among these PC bilayers is discussed in connection with the molecular structure of the PC molecules. Furthermore, the solvent effect on the phase behavior is also described briefly.

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Section: Effect Of Acyl Chain Structure On the Phase Behavior Of Pmentioning

confidence: 99%

Thermotropic and Barotropic Phase Behavior of Phosphatidylcholine Bilayers

Matsuki

Goto

Tada

et al. 2013

IJMS

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“…Similar results were observed when the perilipinenriched LD fraction was compared with the LD from which it was derived (Table 3). Since unsaturation in the fatty acyl chains increases rotational mobility (fluidity) upon insertion of the first double bond (27,54,100), enrichment of saturated FAs and depletion of unsaturated FA and monounsaturated FAs, along with increased cholesterol and sphingolipid content in the perilipin-enriched LD fraction, suggested increased lipid order (rigidity) in the PL monolayer.…”

Section: E995mentioning

confidence: 99%

The phospholipid monolayer associated with perilipin-enriched lipid droplets is a highly organized rigid membrane structure

Storey¹,

McIntosh²,

Senthivinayagam

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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(LD) in lipid metabolism, cell signaling, and membrane trafficking is increasingly recognized, yet the role of the LD phospholipid monolayer in LD protein targeting and function remains unknown. To begin to address this issue, two populations of LD were isolated by ConA sepharose affinity chromatography: 1) functionally active LD enriched in perilipin, caveolin-1, and several lipolytic proteins, including ATGL and HSL; and 2) LD enriched in ADRP and TIP47 that contained little to no lipase activity. Coimmunoprecipitation experiments confirmed the close association of caveolin and perilipin and lack of interaction between caveolin and ADRP, in keeping with the separation observed with the ConA procedure. The phospholipid monolayer structure was evaluated to reveal that the perilipin-enriched LD exhibited increased rigidity (less fluidity), as shown by increased cholesterol/phospholipid, Sat/Unsat, and Sat/ MUFA ratios. These results were confirmed by DPH-TMA, NBDcholesterol, and NBD-sphingomyelin fluorescence polarization studies. By structure and organization, the perilipin-enriched LD most closely resembled the adipocyte PM. In contrast, the ADRP/TIP47-enriched LD contained a more fluid monolayer membrane, reflecting decreased polarizations and lipid order based on phospholipid fatty acid analysis. Taken together, results indicate that perilipin and associated lipolytic enzymes target areas in the phospholipid monolayer that are highly organized and rigid, similar in structure to localized areas of the PM where cholesterol and fatty acid uptake and efflux occur. lipolysis; caveolae; caveolin; concanavalin-A; adipose differentiationrelated protein IT IS INCREASINGLY CLEAR THAT LIPID DROPLETS (LD) are dynamic organelles with regulatory roles in many cellular processes besides lipid metabolism, including cell signaling, immune function, membrane trafficking, and regulation of longevity (42,70). Whereas the mechanism of these processes is only partially understood, much less is known about how the structure of the LD phospholipid monolayer may affect LD protein targeting and function. Embedded in the monolayer that surrounds the LD neutral lipid (NL) core are proteins, such as perilipins (65) and adipose differentiation-related protein (ADRP) (16,41), that coat the LD surface and lipids such as cholesterol (4, 80) that help to define the LD structure. Perilipin and ADRP are two members of the PAT [perilipin, ADRP, tail-interacting protein 47 (TIP47)] family from the Plin group of genes (56) that were initially thought to act simply as barriers to protect the NL core against lipolytic action (63, 65). Current evidence suggests a more complex mechanism, one that requires coordination of perilipin with several lipases, including adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and the activator molecule comparative gene identification-58 (CGI-58), a protein with no lipolytic activity that increases ATGL activity to promote triacylglyerol hydrolysis (11,14,44). Perilipin and HSL upon hormonal stimu...

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“…see Hamamoto et al, 1994) indicates they are likely to be part of a homeoviscous adaptive response to regulate membrane fluidity, However, it has been demonstrated using model lipid systems that the introduction of more than two double bonds in a membrane lipid has little additional effect on lowering the liquid-crystalline to gel phase transition temperature and even monounsaturated phospholipids of the type found commonly in bacterial membranes remain fluid at temperatures a few degrees below zero (Russell, 1989). In fact the introduction of multiple double bonds restricts acylchain rotation and creates a structural element that influences the packing order of the acyl chains, because each double bond 'bends' the acyl chain so that molecules with four or five double bonds form ' hairpins ' which reduce the effective acyl chain length (Keough & Kariel, 1987;Keough et al, 1987). The presence of PUFAs may be more relevant to the maintenance of the correct phase of the lipids (i.e.…”

Section: Role Of Pufas In Bacterial Membranesmentioning

confidence: 99%