The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Abstract

In recent years, research of antitumor activity of natural compounds isolated from plant material has increased. Polyphenols have gained significant attention due to their proapoptotic abilities and their involvement in migration and inhibition of metastasis processes. The anticancer effects of polyphenolic extracts of Viburnum opulus fruit against human breast (MCF-7) and cervical (HeLa) cancer cell lines have been confirmed in this study. It was demonstrated that the tested preparations (methanol – M and acetone – A from pomace, juice – J and juice after extraction to the solid phase SPE – PF) show cytotoxic activity and regulate the migration process of cancer cells. The degree of inhibition of cell migration was measured at two times - 24 h and 48 h after addition of the tested preparations. The highest toxicity towards both cell lines was demonstrated by the polyphenol fraction obtained after juice purification SPE (IC50 values at concentration of 63,541 and 19,380 μg/mL for HeLa and MCF cell lines, respectively). At the same time, the same preparation inhibited cell migration the most (nearly 70% compared to controls at both times at the concertation of 15 and 30 μg/mL). All preparations showed the antioxidant ability, but the Viburnum opulus juice (200 and 350 g/mL) and the preparation after its purification (15 and 30 μg/mL) have larger ability to inhibit the intracellular oxidative stress (30-40%) than preparation obtained from pomace (nearly by 20% at concentration of 20 and 50 μg/mL of M and A). Despite the antioxidative capacity of the preparations, they simultaneously decreased cellular mitochondrial potential. The results obtained indicate the high potential of components of Viburnum opulus polyphenolic compounds can be used in the production of innovative dietary supplements or pharmacological preparations for people with an increased risk or inclination towards developing breast or cervical cancer.

The Effects of Trifolium pratense L. Sprouts’ Phenolic Compounds on Cell Growth and Migration of MDA-MB-231, MCF-7 and HUVEC Cells

“…To determine the effects of extracts on cellular migration, Oris™ Cell Migration Assays were done according to the manufacturer's protocol with modifications [16]. Briefly, cells were seeded on the 96-well plate with "stopper" barriers that create a central cell-free detection zone for cell migration experiments.…”

“…To evaluate red clover seed sprouts' chemopreventive properties, we treated cells with t-BOOH, which generates oxidative stress under in vitro conditions via production of tertbutoxyl, peroxyl, alkoxyl and methyl radicals catalyzing lipid peroxidation, DNA strand breaks and alteration in intracellular calcium homeostasis [14,16]. As compared to the untreated cells, t-BOOH lowered cells metabolic activity by almost 20%-30% (Table 3).…”

“…The second main T. pratense isoflavone, biochanin A, has an impact on membrane stiffening [37]. Because mitochondrial dysfunction can promote progression of a cancer to an apoptosisresistant/chemo-resistant and/or invasive phenotype, we have studied the effect of extracts on mitochondrial membrane potential (MMP) [16,25]. Though extracts dropped intracellular ROS level, surprisingly, we observed an almost 15% decrease of MMP in MDA-MB-231 cells preincubated with CUF and CUFH at IC0 ( Figure 4B).…”

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

“…The effects of the extracts on cellular migration was evaluated with a modified scratch-wound assay, which is based on measuring the free detection zone: the standardized round-shaped area decreases with cell migration [ 51 ]. Briefly, cells were seeded overnight on 96-well plates with “stopper” barriers that created a central cell-free detection zone for the cell-migration experiments.…”

Valorization of European Cranberry Bush (Viburnum opulus L.) Berry Pomace Extracts Isolated with Pressurized Ethanol and Water by Assessing Their Phytochemical Composition, Antioxidant, and Antiproliferative Activities

“…Therefore berry-rich diets are considered as effective means in disease prevention and even their treatment. Earlier studies on V. opulus reported its antioxidant activity [ 5 ] and strong radical scavenging capacity, mainly due to a high phenolic concentration [ 10 ], as well as anticancer activity [ 11 , 12 , 13 ]. For instance, Ceylan et al [ 14 ] reported that the extracts of V. opulus fruits exhibit in vitro and in vivo anticancer activity against Ehrlich ascites carcinoma (EAC) bearing mice, and these effects were related to alkaloids, phenolic compounds, flavonoids, and/or their synergistic effects.…”