The Influence of Vitamins A, B or D, Anemia or Fasting Upon the Rate of Fat Absorption in the Rat

Irwin, Margaret House; Steenbock, H.; Kemmerer, A. R.; Weber, Janet

doi:10.1093/jn/12.4.357

Cited by 8 publications

(1 citation statement)

References 9 publications

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“…In addition, the very active turnover rate of the epithelial lining of the intestine (11) imposes a continuous need for replenishment of structural protein. Recently we have shown a very close correlation between the level of amino acid-activating enzymes (AAAE) in homogenates of rat intestinal epithelium and prolonged fasting (12), a condition known to be associated with impaired intestinal absorption of fat (13,14). Since AAAE initiate the sequence of reactions involved in the synthesis of protein (15), the above observation suggested the possibility that substances having an inhibitory effect on the synthesis of protein may similarly impair the intestinal absorption of lipids.…”

Section: Introductionmentioning

confidence: 99%

Effect of dl-ethionine on the intestinal absorption and transport of palmitic acid-1-14C and tripalmitin-14C. Role of intramucosal factors in the uptake of luminal lipids

Kessler¹,

Mishkin²,

Stein³

1969

J. Clin. Invest.

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A B S T R A C T The effect of DL-ethionine on the uptake and transport of lipid by the rat small intestine was investigated. A cottonseed oil emulsion containing 1'C-labeled tripalmitin or palmitic acid was administered intragastrically to rats pretreated with DL-ethiOnine, DLethionine plus methionine, or saline, and the rats were sacrificed 2, 4, and 6 hr later. Lipids from the plasma, the stomach, the colon, the luminal contents of the small intestine, and the wall of the small intestine were extracted, fractionated, and their radioactivity assayed.Ethionine markedly inhibited the uptake of lipids by the small intestine. This inhibition was not related to impairment of intraluminal lipolysis since analagous inhibitions were observed when palmitic acid or predigested triglyceride (TG), obtained through a jejunal fistula from normal animals, was administered instead of tripalmitin. Ethionine also inhibited the transport of lipid from the wall of the small intestine. A significant fraction of the administered lipid remained in the wall of the small intestine, and only a small fraction was transported to the blood stream. Although most of the wall radioactivity was in the form of TG, significant proportions were also found in the free fatty acid (FFA) and partial glyceride fractions, indicating a marked inhibition of mucosal reesterification to TG. were directly related to the degree of inhibition of uptake of luminal radioactivity. This relationship suggests that the rate of reesterification, the level of mucosal FFA, and the rate of transport of intramucosal TG may be of importance in determining the extent of uptake of intraluminal lipid by the mucosal cells.Since a significant fraction of the wall radioactivity was in the form of TG, the decreased transport of wall lipids was attributed to an impairment of chylomicron completion due to inhibition of either the synthesis of chylomicron apoprotein or the association of preformed TG with the protein moiety of chylomicrons. Experiments with labeled amino acids support the first possibility.

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Section: Introductionmentioning

confidence: 99%

Effect of dl-ethionine on the intestinal absorption and transport of palmitic acid-1-14C and tripalmitin-14C. Role of intramucosal factors in the uptake of luminal lipids

Kessler¹,

Mishkin²,

Stein³

1969

J. Clin. Invest.

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Diseases of Metabolism and Nutrition

Wilder¹

1939

Arch Intern Med (Chic)

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Absorptive Capacity of the Jejunum of Obese and Lean Subjects

Billich¹,

Bray²,

Gallagher³

et al. 1972

Arch Intern Med

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The jejunum of eight obese (mean weight 139 kg [306 lb 8 oz]) and four lean subjects (mean weight, 71 kg [156 lb 8 oz]) was perfused with isotonic glucose-sodium chloride solutions containing a nonabsorbable marker polyethylene glycol and, in four subjects, also folic acid, before and after a fi ve\x=req-\ day total caloric fast. Absorption of glucose, water, sodium, chloride, and folic acid and secretion of urea and potassium were determined. The absorptive capacity of the sub-stances measured was identical in normal\x=req-\

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The Influence of Vitamins A, B or D, Anemia or Fasting Upon the Rate of Fat Absorption in the Rat

Cited by 8 publications

References 9 publications

Effect of dl-ethionine on the intestinal absorption and transport of palmitic acid-1-14C and tripalmitin-14C. Role of intramucosal factors in the uptake of luminal lipids

Effect of dl-ethionine on the intestinal absorption and transport of palmitic acid-1-14C and tripalmitin-14C. Role of intramucosal factors in the uptake of luminal lipids

Diseases of Metabolism and Nutrition

Absorptive Capacity of the Jejunum of Obese and Lean Subjects

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