The influence on biotissue laser resection of a strongly absorbing layer at the optical fiber tip

Kuznetsova, Daria; Elagin, Vadim; Karabut, Maria; Shakhova, Maria; Bredikhin, V. I.; Snopova, Liudmila; Шахов, А. В.; Sapogova, Natalia; Bityurin, N.; Баграташвили, В. Н.; Kamensky, Vladislav A.

doi:10.1142/s1793545816500115

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…13 According to a previous report, resections made using a laser scalpel with a bare tip led to the formation of a wide region (nearly 300 mm) of thermally coagulated tissue. 22 The thermal response of pancreatic cancer cells has been investigated in vitro. Exposure to 50 C for 5 minutes resulted in a significant decline in cell viability (70%) on day 1 with a continued decline to 0% by day 7 following the exposure.…”

Section: Discussionmentioning

confidence: 99%

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Can “Indirect” Contact Laser Surgery be Used for Fluorescence-Image Guided Tumor Resections? Preliminary Results

Elagin

Shakhova

Sirotkina

et al. 2018

Technol Cancer Res Treat

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Ensuring the complete removal of tumor tissue is the main challenge during resection operations. Recently, a technique of “indirect” contact laser surgery has been developed. In this study we assess the possibility of using such surgery for fluorescence image-guided tumor resection. Mouse colon adenocarcinoma CT-26 cells stably expressing the fluorescent protein mKate-2 was used as the tumor model. Resections of the tumor nodes were performed with either a scalpel blade, a laser scalpel with a bare tip, or a laser scalpel with a strongly absorbing coating on the fiber tip. Tumor-positive resection margins were detected using an IVIS Spectrum fluorescence imaging system. After tumor resection with the scalpel blade over half of the animals needed one additional resection to remove residual tumor cells. Animals in this group showed tumor recurrence within 7 days. Fluorescence imaging of the tumor bed, performed after resection to assess the presence of tumor cell clusters, was sufficiently effective only with a bloodless resection. The laser scalpels both with the bare tip and with the strongly absorbing coating on the tip provided such bloodless tumor resection in contact mode. Fewer animals required additional resections when the bare tipped scalpel was used and this also resulted in a reduction in tumor recurrence. After resections were carried out with the laser scalpel with the strongly absorbing coating on the tip, fluorescence was detected in the operative field and this led to undertaking additional resections, although subsequent investigation suggested that this was “false” fluorescence, resulting from the effects of the scalpel rather than the presence of residual tumor cells. The method of laser resection with a strongly absorbing coating on the tip therefore did not appear to demonstrate definite advantages over laser resection with a bare tip when removing tumors.

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Section: Discussionmentioning

confidence: 99%

“…A previous study had demonstrated the efficacy of this laser parameter for skin resection. 22 The laser light was delivered to the tissue via silica fiber 550 μm in diameter. A fiber tip cleared from the cladding layer was used for resection.…”

Section: Methodsmentioning

confidence: 99%

Can “Indirect” Contact Laser Surgery be Used for Fluorescence-Image Guided Tumor Resections? Preliminary Results

Elagin

Shakhova

Sirotkina

et al. 2018

Technol Cancer Res Treat

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“…In recent years, the progress in laser surgery has been mainly associated with the approbation of newly created laser sources [1][2][3] and the use of novel focusing elements [4] or coatings [5][6][7]. The effect of the laser radiation on a biological tissue has been theoretically considered in Refs [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Model for indirect laser surgery

Sapogova

Bredikhin

Bityurin

et al. 2016

Biomed. Opt. Express

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Abstract:We present a theoretical model for laser cutting of biological tissue by a strongly heated fiber tip with a highly absorbing coating. A significant dependence of the cutting speed and cutting depth on the inclination angle of the scalpel to the surface when scattering exceeds absorption in the biological tissue is shown. Experimental evidences of this effect are presented. In the experiment, we used silica fiber with coating made of carbon and silicon organic varnish, the 0.97-µm wavelength laser and porcine skin. The additional opportunity to increase the efficiency of cutting by deposition of the absorbing layer on the tissue surface is considered.

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