The influences of hyperbaric oxygen therapy with a lower pressure and oxygen concentration than previous methods on physiological mechanisms in dogs

Abstract

Recently, hyperbaric oxygen therapy with a lower pressure and oxygen concentration (L-HBOT) than previous methods has been used for dogs in Japan; however, the influences of L-HBOT on dogs have not been clarified. To verify the influences of L-HBOT on physiological mechanism in dogs, we investigated blood gas parameters, glutathione peroxidase (GPx) activity, heart rate variability, stress-related hormones and skin conductance (SC) in 4 clinically normal beagle dogs with catheters in their carotid arteries and jugular veins when they were quiet, after running, after receiving L-HBOT (30% oxygen concentration, 1.3 atmospheres absolute, 30 min) or after not receiving L-HBOT. The results showed there were no changes in blood gas parameters, heart rate variability and catecholamine levels after L-HBOT. GPx activity was significantly higher, and the SC and cortisol level were lower in dogs that received L-HBOT than those when they were quiet. These results suggested that L-HBOT may have a small influence on oxygenation dynamics, activate antioxidant enzymes such as GPx, restrain autonomic nervous activity and control the balance between oxidation and antioxidation inside the body.

Effects of hyperbaric oxygen therapy on uncomplicated incisional and open wound healing in dogs

“…Species and number of colony‐forming units (CFU) were recorded for each culture. A bacterial load score (BLS) was identified and assigned for each cultured bacteria as previously described: 0 = no growth or isolated from broth culture only, 1 = <50 CFU, 2 = 50‐100 CFU, and 3 = >100 CFU …”

“…These previously reported indications have provided only subjective evidence of benefit from HBOT in dogs, with no comparison or controlled group investigation . Investigative evidence regarding the efficacy of this therapy for any type of wound healing in dogs is lacking; however, recent data have been published on its safety and physiologic effects of improved tissue and arterial and transcutaneous oxygenation . Currently, use of HBOT as an adjunct to any wound healing in dogs is based on subjective or anecdotal evidence evaluating complicated wounds in dogs, and research studies evaluating complicated wounds in rabbits, rats, and man .…”

“…7,17,18,[26][27][28] Investigative evidence regarding the efficacy of this therapy for any type of wound healing in dogs is lacking; however, recent data have been published on its safety and physiologic effects of improved tissue and arterial and transcutaneous oxygenation. 17,29,30 Currently, use of HBOT as an adjunct to any wound healing in dogs is based on subjective or anecdotal evidence evaluating complicated wounds in dogs, 17,18,[26][27][28] and research studies evaluating complicated wounds in rabbits, rats, and man. 5,6,[19][20][21][22][23][24] The major deficit in the veterinary literature is the lack of prospective, randomized evaluations of the effects of HBOT on wound healing in dogs.…”

Effects of mild hyperbaric oxygen therapy on timing sequence recovery of muscle fatigue in Chinese university male athletes

“…Nuclear factor erythroid 2-related factor 2 (NRF2) is believed to play a crucial role in MHOT's cellular defense mechanism against oxidative stress. 55 It is suggested that MHOT interventions may aid in the removal of free radicals, thereby preventing potential apoptotic pathways triggered by oxygen-derived free radicals and therefore, exerting a protective effect. Typically administered daily for 1 to 2 h at pressures ranging from 2.0 to 2.8 ATA, HBOT is commonly utilized over 3 to 10 sessions for sports-related injuries.…”

Examining the Effects of Hyperbaric Oxygen Therapy on the Cardiovascular System and Oxidative Stress in Insulin-Treated and Non-Treated Diabetic Rats

“…Also, it has been demonstrated that 30 min after HBOT with 30% oxygen pressure at 1.3 ATA in dogs, GPx activity in erythrocytes increased. The nuclear factor erythroid 2-related factor 2 (Nrf2), a redox-sensitive transcription factor, is thought to play a key role in the cellular defense against oxidative stress by upregulating the transcription of antioxidant stress proteins like GPx and phase II defense enzymes [ 28 ]. Additionally, in the liver tissues of STZ-induced diabetic rats, oxidative-stress-related changes in the mRNA and protein expression of antioxidant enzymes, such as copper-zinc superoxide dismutase (Cu-Zn SOD) and catalase (CAT), have been found [ 29 ].…”