“…It is of some interest to compare the limiting aw of c. 0.945 in the present experiments with the approximate aw's which may be deduced from the limiting conditions reported by other workers. Severens and Tanner (1945) found no growth of unadapted salmonellae above 3 per cent. NaCI, but after adaptation growth occurred in up to 8 per cent.…”
Section: Discussionmentioning
confidence: 85%
“…The results reported in this paper have been obtained with unadapted cultures and no attempt has been made to determine whether salmonellae could be adapted or selected to grow at even lower aw's. Although Severens and Tanner (1945) reported that salmonellae acquired permanently increased tolerance to NaCI as a result of selection, the maximum concentration of salt tolerated after adaptation was 8 per cent. (aw 0.95).…”
Section: Discussionmentioning
confidence: 99%
“…Similar studies do not appear to have been made previously, although some authors have studied the inhibition of growth by solutes such as sodium chloride. Severens and Tanner (1945) reported that 3 per cent. NaCl was the highest concentration permitting growth of unadapted strains of S.…”
Sixteen strains of salmonellae have been grown in various media of known water activity (a..,) at 30�C. The reactions of'15 motile strains were very similar, whereas the single non-motile strain grew more slowly and over a smaller range of a",'s.
“…It is of some interest to compare the limiting aw of c. 0.945 in the present experiments with the approximate aw's which may be deduced from the limiting conditions reported by other workers. Severens and Tanner (1945) found no growth of unadapted salmonellae above 3 per cent. NaCI, but after adaptation growth occurred in up to 8 per cent.…”
Section: Discussionmentioning
confidence: 85%
“…The results reported in this paper have been obtained with unadapted cultures and no attempt has been made to determine whether salmonellae could be adapted or selected to grow at even lower aw's. Although Severens and Tanner (1945) reported that salmonellae acquired permanently increased tolerance to NaCI as a result of selection, the maximum concentration of salt tolerated after adaptation was 8 per cent. (aw 0.95).…”
Section: Discussionmentioning
confidence: 99%
“…Similar studies do not appear to have been made previously, although some authors have studied the inhibition of growth by solutes such as sodium chloride. Severens and Tanner (1945) reported that 3 per cent. NaCl was the highest concentration permitting growth of unadapted strains of S.…”
Sixteen strains of salmonellae have been grown in various media of known water activity (a..,) at 30�C. The reactions of'15 motile strains were very similar, whereas the single non-motile strain grew more slowly and over a smaller range of a",'s.
“…Cells in fresh culture medium and in contact with K C S for 1 hour before test. V. Fresh culture medium contained 2 X M KCN for [8][9][10] hours, then cells added to give 1 O P M KCN and usual cell density. VI.…”
Section: Table I the Effect Of Various Conditions Upon Respiration Bmentioning
SUMMARY. Cells from a clonal culture of Tetrahymena pyriformis W were cultured in the presence of 10‐4 M KCN for a prolonged time until they became “adapted” to cyanide. The effect of 10‐3 M KCN upon respiration in the “adapted” cells was compared with the effect upon “normal” cells of the same clone grown in the absence of cyanide. The direct method of Warburg was employed.
Adapted cells are more resistant against respiratory inhibition than are normal cells. Resistance is due both to a factor or factors retained within the cell and the extracellular factor(s) released into the medium by the adapted cells during their growth. The intracellular factor is acid labile, and apparently may be developed within a short time. The extracellular factor is heat labile and may cause normal cells to be more resistant against respiratory inhibition by KCN.
Resistance to respiratory inhibition is lost if adapted cells are cultured in the absence of KCN. It is proposed that changes involved in gain or loss of cyanide resistance are due to physiological adaptation and not to the usual genetic influences.
“…Certain color mutants of Drosophila also vary in their resistance to thiourea (Goldsmith and Harnly, 1946). Mutation in microorganisms leads to increased resistance to bacterial viruses (Luria, 1947;Demerec and Latarjet, 1947), to heavy metal salts (Severens and Tanner, 1945), and to penicillin (Demerec, 1945).…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.