The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

Abstract

In recent years, probiotics and their derivatives have been recognized as important therapeutic agents in the fight against cancer. Therefore, this study aimed to investigate the anticancer effects of membrane vesicles (MVs) from Lentilactobacillus buchneri strain HBUM07105 probiotic isolated from conventional and unprocessed yogurt in Arak province, Iran, against gastric and colon cancer cell lines. The MVs were prepared from the cell-free supernatant (CFS) of L. buchneri and characterized using field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) and SPS-PAGE techniques. The anticancer activity of MVs was evaluated using MTT, flow cytometry, qRT-PCR techniques, and a scratch assay. The study investigated the anti-adenocarcinoma effect of MVs isolated from L. buchneri on a human gastric adenocarcinoma cell line (AGS) and a human colorectal adenocarcinoma cell line (HT-29) at 24, 48, and 72-h time intervals. The results demonstrated that all prepared concentrations (12.5, 25, 50, 100, and 200 µg/mL) of MVs reduced the viability of both types of human adenocarcinoma cells after 24, 48, and 72 h of treatment. The analysis of the apoptosis results revealed that the percentage of AGS and HT-29 cancer cells in the early and late stages of apoptosis was significantly higher after 24, 48, and 72 h of treatment compared to the untreated cancer cells. After treating both AGS and HT-29 cells with the MVs, the cells were arrested in the G0/G1 phase. These microvesicles demonstrate apoptotic activity by increasing the expression of pro-apoptotic genes (BAX, CASP3, and CASP9). According to the scratch test, MVs can significantly decrease the migration of HT-29 and AGS cancer cells after 24, 48, and 72 h of incubation compared to the control groups. The MVs of L. buchneri can also be considered a potential option for inhibiting cancer cell activities.

In vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells

“…This retention of adenocarcinoma cells in a pre-synthetic period is advantageous because it would provide an opportunity to restore the euploid cell population by suppressing the division of aneuploid cells. A similar possibility was reported by other authors, who studied the influence of different probiotics on cervical or intestinal epithelium [26,27]. Like us, other authors have found an increase in the proportion of cells in the G0/G1 period of the cell cycle.…”

Staphylococcus Aureus Membrane Vesicles Kill Tumor Cells Through a Caspase-1-Dependent Pyroptosis Pathway

“…S. aureus MVs can load a variety of pore-forming toxins, such as alpha-hemolysin and leukocidin-like proteins, which may play a role in cytotoxicity. 21 LDH release assays were performed to evaluate the toxicity of S. aureus MVs against tumor cells. The results showed that LDH release increased in a dose-dependent manner after treatment with different concentrations of the MVs ( Figure 3C and D ).…”

“…Lentilactobacillus buchneri strain HBUM07105-produced MVs can reduce the viability of human gastric adenocarcinoma AGS cells and colorectal adenocarcinoma HT-29 cells in vitro. 21 However, the mechanisms for Gram-positive bacterial MVs killing of tumor cells are unclear. Uncertain components in bacterial MVs may induce apoptosis in the tumor cells.…”

“…Uncertain components in bacterial MVs may induce apoptosis in the tumor cells. 20 , 21 The hemolysin-loaded pneumococcal MVs can be internalized by A549 lung adenocarcinoma cells, while the production of proinflammatory cytokines is irrespective of pneumolysin content. 22 In this study, MVs of Staphylococcus aureus USA300 were prepared and characterized.…”