The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA

Liu, Sijin; Zhang, Daoqiang; Sui, Xiu-Mei; Zhang, Lin; Zi-wei, Cai; Sun, Liqiu; Liu, Yajun; Xue, Yan; Hu, Guo-Fa

doi:10.2478/s11658-007-0031-0

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…Reconstructing the networks of interactions of the various cellular components as enzyme activities and complexes, gene expression, metabolite pools or pathway flux modes is therefore possible. However, capturing the interactions of network elements requires experimental setups with a variety of conditions [90,[95][96][97][98][99]. The ultimate goal of systems biology in the context of plant research is to understand the molecular principles governing plant responses and consistently explain plant physiology [90,100,101].…”

Section: Resultsmentioning

confidence: 99%

The interactome: Predicting the protein-protein interactions in cells

Plewczyński

Ginalski

2009

Cellular and Molecular Biology Letters

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Abstract:The term Interactome describes the set of all molecular interactions in cells, especially in the context of protein-protein interactions. These interactions are crucial for most cellular processes, so the full representation of the interaction repertoire is needed to understand the cell molecular machinery at the system biology level. In this short review, we compare various methods for predicting protein-protein interactions using sequence and structure information. The ultimate goal of those approaches is to present the complete methodology for the automatic selection of interaction partners using their amino acid sequences and/or three dimensional structures, if known. Apart from a description of each method, details of the software or web interface needed for high throughput prediction on the whole genome scale are also provided. The proposed validation of the theoretical methods using experimental data would be a better assessment of their accuracy.

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Section: Resultsmentioning

confidence: 99%

The interactome: Predicting the protein-protein interactions in cells

Plewczyński

Ginalski

2009

Cellular and Molecular Biology Letters

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“…Similarly, antisense human osteopontin RNA has been successfully used to decrease tumor growth in osteosarcoma cells by blocking osteopontin mRNA translation (Liu et al 2008). Antisense oligonucleotides have also been successfully used for inhibiting osteopontin translation in oral cancer cells as was recently demonstrated by Muramatsu et al (2005).…”

Section: Abstract Argatroban · Antisense Human Osteopontin · Anti Ostmentioning

confidence: 97%

Inhibition of osteopontin dependent carcinogenesis

Kapoor¹

2008

J Cancer Res Clin Oncol

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Osteopontin is a molecule that promotes cellular proliferation as well as metastasis and plays a major role in the evolution of most systemic malignancies. Inhibition of carcinogenetic mechanisms that involve osteopontin will undoubtedly go a long way in controlling the growth of these tumors. A number of new molecules such as argatroban agelastatin A and selenium have been identiWed in this regard. For instance, Schulze et al. (in Breast Cancer Res Treat) have recently reported the successful utilization of argatroban for decreasing the growth of breast cancer tumor cells as well as their lymphatic metastasis. Similarly, anti osteopontin antibodies and antisense osteopontin RNA have been successfully used to inhibit tumor growth in prostate carcinomas and osteosarcomas, respectively. There is a clear and urgent need to expand the use of these molecules as well as to further identify other potent inhibitors of osteopontin mediated tumerogenesis.

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“…The importance of S100A4 in OS development has not been thoroughly investigated in patient cohorts; nevertheless, our results are in agreement with gene regulation and expression patterns previously described in vitro and in experimental metastatic models in vivo 3, 13. Similarly, OPN antisense RNA treatment led to reduced tumorigenicity of xenotransplanted OS cells, while the protein did not predict outcome in a study of OS patients 42, 43. Strengthening our hypothesis is, however, data from breast cancer patients where expression of S100A4 was found to have a borderline correlation with OPN, and where both proteins were independent factors of patient survival 44…”

Section: Discussionmentioning

confidence: 65%

Osteopontin—An important downstream effector of S100A4‐mediated invasion and metastasis

Berge

Pettersen

Grotterød

et al. 2011

Intl Journal of Cancer

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Substantial evidence has linked the small calcium-binding protein S100A4 to metastatic progression. S100A4-mediated effects include stimulation of angiogenesis, regulation of cell death and increased cell motility and invasion, but the exact molecular mechanisms by which the protein exerts these effects are incompletely elucidated. In the present study, we demonstrate that S100A4 induces NF-jB-dependent expression and secretion of osteopontin (OPN) in a selection of osteosarcoma cell lines. OPN is, as S100A4, known to result in a variety of cellular effects potentially leading to increased angiogenesis and metastasis, and several of the activated signaling pathways are common for the two proteins. In our study, extracellular S100A4 was found to upregulate enzymes of the plasminogen activator system and matrix metalloproteinase (MMP) family, especially urokinase plasminogen activator and MMP-13. Furthermore, increased motility and invasion was observed in vitro as a result of S100A4 treatment. OPN expression was inhibited by the use of siRNA molecules, and a partial blocking of S100A4-induced effects on protease expression and invasive capacity was detected. In conclusion, our results suggest regulation of OPN as a downstream molecular mechanism of S100A4 signaling. This novel finding adds more information to how S100A4 mediates tumor development and metastatic progression. The observation of a link between S100A4 and OPN, and also identification of common downstream effect molecules, highlights them, their receptors or downstream proteins, as targets for therapeutic approaches.The S100A4 protein belongs to the S100 protein family, a group of small, acidic, calcium-binding proteins with different expression patterns and distinct tissue distributions and biological functions. 1 Over the years, numerous reports have convincingly linked expression of S100A4 to the invasive and metastatic capacity of cancer cells. 2,3 The protein has been associated with more aggressive and invasive tumors and increased metastatic potential in transgenic animals. 4,5 In addition, clinical evidence has clearly indicated a correlation between augmented S100A4 expression and poor prognosis in a number of human cancers, and S100A4 has been proposed as a potential marker predicting metastasis and survival. 6,7 The biological function and molecular mechanisms by which S100A4 exerts its putative metastasis-promoting effects are largely unknown, and the protein is most likely involved in several facets of tumor progression. Moreover, both intracellular and extracellular S100A4 have been shown to promote cancer development. The protein has been demonstrated to interact with cytoskeletal proteins, suggesting a possible role in cell motility, 8 and extracellular S100A4 has been identified as a potent inducer of angiogenesis. 9 Importantly, an association between exposure to, or expression of, S100A4 and members of the matrix metalloproteinase (MMP) family is well documented, 10-13 and as a result, S100A4 is suggested to promote progression of c...

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The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA

Cited by 12 publications

References 28 publications

The interactome: Predicting the protein-protein interactions in cells

The interactome: Predicting the protein-protein interactions in cells

Inhibition of osteopontin dependent carcinogenesis

Osteopontin—An important downstream effector of S100A4‐mediated invasion and metastasis

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