The inhibition of mitochondrial metabolic activity in etiolated pea seedlings under water stress

Генерозова, И. П.; Maevskaya, S. N.; Шугаев, А. Г.

doi:10.1134/s1021443709010063

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…Plants also need energy under stress and the relative importance of the mitochondria as the principal organelle for ATP production increases [18]. While other work has shown that under intense water stress, mitochondria activity decreases [19], some have reported an increase in mitochondrial function under moderate, slow onset stress [20]. Water stress generates ethylene, and would also therefore generate cyanide.…”

Section: Discussionmentioning

confidence: 99%

The β-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana

Machingura

Sidibe

Wood

et al. 2013

Plant Physiology and Biochemistry

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The β-cyanoalanine pathway is primarily responsible for detoxification of excess cyanide produced by plants. Recent evidence suggests that cyanide detoxification via this pathway may be involved in the response and tolerance to water deficit in plants. The aim of this study was to explore this role in Arabidopsis thaliana in greater detail. The first objective was to establish responsiveness of the pathway to the magnitude and duration of water deficit. The second objective was to examine how interruption of single genes (AtCysA1, AtCysC1 and AtNIT4) encoding enzymes of the pathway influenced the ability to metabolize cyanide and withstand water deficit. Arabidopsis plants were exposed to conditions which emulated acute and chronic water deficit, followed by measurement of tissue cyanide concentration, activity of enzymes, and physiological parameters. The results for wild-type Arabidopsis demonstrated a transient increase in cyanide concentration and β-cyanoalanine synthase activity, followed by a decrease in both. The increase in enzyme activity was localized to the tissue in direct proximity to the stress. The knockdown AtCysA1 mutant did not differ from wild-type while AtCysC1 mutants were slightly more sensitive to water deficit. The AtNIT4 mutant was the most sensitive showing decreased growth along with altered chlorophyll content under water deficit as compared to wild-type. Collectively, the results indicated that the pathway is responsive to water deficit although the severity of stress did not alter the nature of the response, implying that the capacity to remove cyanide generated during water deficit may contribute to tolerance to this stress in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

The β-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana

Machingura

Sidibe

Wood

et al. 2013

Plant Physiology and Biochemistry

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“…It has long been known that mt function is plastic in plants. For example, pea seedlings grown under water stress had lower RCRs than controls (Generozova et al ., 2009) and AOX flux control also varies with environment (Fiorani et al ., 2005; Vanlerberghe, 2013; Ahanger et al ., 2017). Given our relatively low sample sizes and the large amount of within-accession (i.e., within genotype) variation seen in basic plant fitness metrics (Fig.…”

Section: Discussionmentioning

confidence: 99%

Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes

et al. 2018

Preprint

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Angiosperm mitochondrial (mt) genes are generally slow-evolving, but multiple lineages have undergone dramatic accelerations in rates of nucleotide substitution and extreme changes in mt genome structure. While molecular evolution in these lineages has been investigated, very little is known about their mt function. Here, we develop a new protocol to characterize respiration in isolated plant mitochondria and apply it to species of Silene with mt genomes that are rapidly evolving, highly fragmented, and exceptionally large (∼11 Mbp). This protocol, complemented with traditional measures of plant fitness, cytochrome c oxidase activity assays, and fluorescence microscopy, was used to characterize inter-and intraspecific variation in mt function. Contributions of the individual “classic” OXPHOS complexes, the alternative oxidase, and external NADH dehydrogenases to overall mt respiratory flux were found to be similar to previously studied angiosperms with more typical mt genomes. Some differences in mt function could be explained by inter-and intraspecific variation, possibly due to local adaptation or environmental effects. Although this study suggests that these Silene species with peculiar mt genomes still show relatively normal mt function, future experiments utilizing the protocol developed here can explore such questions in a more detailed and comparative framework.

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“…Водний дефіцит є одним із найпоширеніших факторів навколишнього сере довища, який здійснює негативний вплив на ріст і продуктивність рослин [4]. У цьому разі адаптація рослин до посухи пов'язана не лише зі структурними перебудовами в клітині, але й із особливостями обміну речовин, а саме з нагромадженням активних низькомолекулярних сполук [10].…”

Section: вступunclassified

“…Останніми роками значна увага приділяється також ролі гормоноподібних речовин в індукуванні стійкості рослин до різноманітних стресових факторів. До таких сполук належить і саліцилова кислота (СК), яка бере активну участь у підвищенні стійкості рослин різних таксономічних груп до високої температури та дефіциту вологи [4].…”

Section: вступunclassified

Influence of salicylic acid on the amount of free aminoacids and proline in plants of wheat and corn under drought conditions

Malenka¹,

Kobyletska²,

Терек³

2014

Biol. Stud.

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The inhibition of mitochondrial metabolic activity in etiolated pea seedlings under water stress

Cited by 15 publications

References 26 publications

The β-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana

The β-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana

Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes

Influence of salicylic acid on the amount of free aminoacids and proline in plants of wheat and corn under drought conditions

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