The inhibition of motility that results from contact between two oligodendrocytes in vitro can be blocked by pertussis toxin

Moorman, Stephen J.

doi:10.1002/(sici)1098-1136(199603)16:3<257::aid-glia8>3.0.co;2-z

Cited by 12 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Disruption of the cytoskeleton might then reflect inhibitory effects of OL–OL contact at high densities where GSLs in apposed membranes of different cells could interact. Addition of isolated myelin fragments or OL “ghosts” to immature OLs in culture caused an increase in intracellular Ca +2 , a decrease in motility of OL processes, and a collapse of fine structure of OLs at the point of contact, also suggestive of an inhibitory effect of cell‐cell contact (Moorman and Hume, 1994; Moorman, 1996). The immature OLs used in those studies had lower GalC expression than more mature ones, and the results observed may have been due to interaction of the added myelin with membrane constituents other than GSLs.…”

Section: Discussionmentioning

confidence: 99%

Effect of liposomes containing cerebroside and cerebroside sulfate on cytoskeleton of cultured oligodendrocytes

Boggs

Wang

2001

J of Neuroscience Research

View full text Add to dashboard Cite

Oligodendrocytes (OLs) and the myelin produced by them are enriched in two glycosphingolipids, galactosylceramide (GalC) and its sulfated form, cerebroside sulfate (CBS). We showed earlier that these two glycolipids in opposed liposomal membranes or in methanol solution can adhere to each other. Here we have examined the potential effect of an interaction between GalC/CBS in apposed membranes of oligodendrocytes (OLs) by incubating cultured OLs with GalC/CBS-containing liposomes and observing the effect on the membrane sheets produced by OLs and on the distribution of OL constituents using fluorescent antibodies and confocal microscopy. The GalC/CBS-containing liposomes caused redistribution or a decrease in the density of anti-GalC and anti-MBP staining but had no effect on the density or distribution of staining by anti-PI(4,5)P(2) that remained uniformly distributed in the membrane sheets. There was no apparent change in the area of the membrane sheets nor in the amount of MBP in OL membranes, as determined by slot blots. In addition, the GalC/CBS-containing liposomes caused depolymerization of microtubules and actin filaments suggesting that the interaction of GSL-containing liposomes with the extracellular surface of the OL caused transmission of a signal across the membrane. Because these two glycolipids can adhere to each other across apposed membranes, the liposomal glycolipids may be interacting with a GalC/CBS-enriched signaling domain in the OL plasma membrane.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of liposomes containing cerebroside and cerebroside sulfate on cytoskeleton of cultured oligodendrocytes

Boggs

Wang

2001

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…Subsequent studies confirmed that CNS myelin and mature oligodendrocytes contain molecular components that restrict axonal regeneration [58][59][60][61]. Several proteins expressed by oligodendrocytes have been identified as myelin-associated inhibitors on the basis of their ability to inhibit neurite outgrowth and induce growth cone collapse.…”

Section: Molecular Determinants Involved In the Dampening Of The Plasmentioning

confidence: 99%

The Experimental Manipulation of Visual Cortex Efferents

Gerrikagoitia¹,

Rienda²,

Martínez‐Millán³

2012

Visual Cortex - Current Status and Perspectives

View full text Add to dashboard Cite

Differentiating oligodendrocytes inhibit neuronal growth cone motility in different ways

Moorman

Gould

1997

J. Neurosci. Res.

View full text Add to dashboard Cite

Oligodendrocyte-specific proteins are thought to limit regeneration by inhibiting motility of the neuronal growth cone. These inhibitory proteins are among an array of molecules, each expressed in a stage-specific fashion as an oligodendrocyte differentiates. Here we show that neuronal growth cone response to contact with differentiating oligodendrocytes does not strictly correlate with the expression of inhibitory proteins. This suggests that the neuronal growth cone response depends on the context in which inhibitory molecules are encountered. In addition, we provide evidence that there are at least two cellular pathways, within the same growth cones, that lead to growth cone collapse.

show abstract

The inhibition of motility that results from contact between two oligodendrocytes in vitro can be blocked by pertussis toxin

Cited by 12 publications

References 22 publications

Effect of liposomes containing cerebroside and cerebroside sulfate on cytoskeleton of cultured oligodendrocytes

Effect of liposomes containing cerebroside and cerebroside sulfate on cytoskeleton of cultured oligodendrocytes

The Experimental Manipulation of Visual Cortex Efferents

Differentiating oligodendrocytes inhibit neuronal growth cone motility in different ways

Contact Info

Product

Resources

About