The inhibition of pectin oligosaccharides on degranulation of RBL-2H3 cells from apple pectin with high hydrostatic pressure assisted enzyme treatment

Allergenicity evaluation of five types of commercial food-derived oligopeptide products

“…RBL-2H3 cell degranulation assay was performed as previously described with slight modifications. 27 RBL-2H3 cells (5 × 10 5 cells per mL, 200 μL per well) were inoculated into a 96-well plate. 24 h later, the cells were washed with PBS, and then 100 μL sera diluted with minimum essential medium without fetal bovine serum was added to each well.…”

Extraction and characterization of a pectin from sea buckthorn peel

“…The degree of esterification (SBHMP) of SBHMP was determined by the method of Ma et al ( 17 ). The DE was calculated using the following Equation:…”

Recent advances in the production of oligogalacturonides and their biological properties

“…Several approaches have been described in the literature for pectin depolymerization, including physical treatments, such as electron beam irradiation, 29 free-radical hydrolysis, 38 physico-chemical processes, 25,37 enzymatic hydrolysis either in submerged systems 8,11,12,15,21–23,35 or with immobilized enzymes 24,31 and even microbial fermentation using wild 10 or genetically modified microorganisms. 32,34 In addition, combined technologies such as enzymatic hydrolysis and high hydrostatic pressure 7 or enzymatic hydrolysis and chemical de-esterification 14 were also employed for the production of di- and tri-galacturonides and mixtures of OGalA with DP1–8, respectively.…”