The inhibitory effect of BSP‐A1/‐A2 on protein kinase C and tyrosine protein kinase

Yu, Bin; Zhao, Yanyan; Zhao, Wei; Chen, Fei; Liu, Ying; Zhang, Jie; Fu, Wei; Zong, Zhi‐Hong; Yu, Alice L.; Guan, Yunqian

doi:10.1002/cbf.1018

Cited by 11 publications

(6 citation statements)

References 27 publications

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“…Indeed, the stimulation of BSPs for capacitation is dependent on exposure time and presence of heparin or high‐density lipoprotein or glycosaminoglycan present in the oviductal fluid (Manjunath and Therien, 2002). On the contrary, Yu et al (2003) have proposed that PDC‐109 also prevents premature acrosome reaction of spermatozoa in the female reproductive tract by inhibiting protein kinase C activity. BSP proteins exert both beneficial and detrimental effects, which are important in maintaining spermatozoa in an appropriate state to facilitate fertilization in the female reproductive tract.…”

Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

169

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“…The major bovine SPPs are BSP-A1/A2, BSP-A3 and BSP-30 kDa which play substantial roles in fertility by maintaining sperm in an appropriate state in the female genital tract until the oocyte reaches the site of fertilisation (Yu et al, 2003;Gwathmey et al, 2006). Our results are partially consistent with these findings because protein bands of 15 and 13.5 kDa which might correspond to BSP-A1 or BSP-A3 and BSP-A2, respectively were the most abundant proteins in these fertile bulls.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, two additional functions were proposed for BSPs. The first one is the activity of protein kinase C (PKC) and of tyrosine protein kinase (TPK) inhibited in vitro by BSP-A1/A2 (Yu et al, 2003). It was proposed that the inhibition of PKC may serve to prevent the premature acrosome reaction of sperm in the female geni-tal tract.…”

Section: Introductionmentioning

confidence: 99%

Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Almadaly¹,

Farrag²,

El-Nasr³

2019

BJVM

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Protein and lipid molecules in seminal plasma (SP) collected from fertile bulls were investigated. Semen was collected from 10 bulls (2 ejaculates each) and examined for standard semen analysis. Raw SP was recovered by centrifugation and total protein (TP) concentration was determined using a refractometer. Raw SP was desalted using a Sephadex G-25 desalting column then both raw and desalted SP was subjected to SDS-PAGE. Neutral lipids and phospholipids of raw and desalted SP were separated by thin-layer chromatography (TLC). The results revealed that, all bulls had normal semen characteristics and TP concentration in SP ranged from 7.0 to 10.4 g/dL except bull No. 6 had a relatively low concentration of 4.9 to 6.8 g/dL. Neither proteins nor lipids species were different between raw and desalted SP. Seventeen proteins were detected ranging from 8.5 to 185.8 kDa, and those of 12, 13.5, 15, 21, 23 and 38 kDa were predominant. Notably, proteins of 10, 17.5, 19, 21, 80 and 185.8 kDa might be new candidates of SP proteins (SPPs). The detected neutral lipid spots corresponded to cholesterol, 1,2-dimyristoyl glycerol, 1,2-dioleoyloglycerol, 1,3-dimyristoyl glycerol and 1,3-dioleoyloglycerol. The detected phospholipids spots corresponded to non-migrating phospholipids, sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylethanolamine (PE), cerebroside and polyglycerol phosphatide. Cholesterol represents the major molecule of neutral lipids, whereas SM, PC, PI and PE represent the major phospholipids. Noteworthy, there were 2 species of diacylglycerol (DAG) and 3 species of PI in bovine SP. In conclusion, this study gave a general picture of SP protein and lipid species in fertile bull semen, which might serve as fundamental knowledge for either semen analysis or prediction of male fertility.

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“…Abundant low molecular weight bovine seminal plasma proteins were replaced during cryopreservation by high molecular weight extender-derived chicken structural proteins. Major bovine seminal plasma proteins PDC-109 (BSP-A1/A2), BSP-A3 and BSP-30 kDa play important roles in fertility by maintaining sperm in an appropriate state in the female tract until the oocyte reaches the site of fertilization [10][11][12][13]. It is unclear whether the effect of processing that decreased the abundance of these BSP proteins by more than half would render them ineffective in preventing premature capacitation and acrosome reaction of sperm from normal fertility bulls in the female tract.…”

Section: Discussionmentioning

confidence: 99%

Characterization of proteins in cryopreserved and non-cryopreserved seminal plasma of dairy bulls of dif-fering fertility

Odhiambo¹,

Dailey²

2011

OJAS

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Seminal plasma is composed of secretions from accessory sex glands, which are mixed with sperm at ejaculation and contribute the majority of semen volume. Seminal plasma is considered a transport and support medium for sperm in the female reproductive tract. Because seminal plasma is not required for fertilization, the importance of its constituents to the establishment of normal pregnancy has been overlooked.

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The inhibitory effect of BSP‐A1/‐A2 on protein kinase C and tyrosine protein kinase

Cited by 11 publications

References 27 publications

Seminal Plasma: An Essential Attribute to Spermatozoa

Seminal Plasma: An Essential Attribute to Spermatozoa

Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Characterization of proteins in cryopreserved and non-cryopreserved seminal plasma of dairy bulls of dif-fering fertility

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