The inhibitory effect of gypenoside stereoisomers, gypenoside L and gypenoside LI, isolated from Gynostemma pentaphyllum on the growth of human lung cancer A549 cells

Xing, Shao‐Fang; Liu, Linhua; Zu, Ma‐Li; Ding, Xiaoyun; Cui, Wei-Ye; Chang, Tuo; Piao, Xiang‐Lan

doi:10.1016/j.jep.2018.03.012

Cited by 24 publications

(12 citation statements)

References 30 publications

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“…Apoptosis is a controlled form of cell death that can lead to various morphological changes, such as nuclear pyknosis, cell surface changes, and chromosome condensation . We examined morphological changes by Hoechst 33258 staining and show that A549 cells treated with SCU display specific apoptotic changes in a concentration‐dependent manner.…”

Section: Discussionmentioning

confidence: 98%

“…Apoptosis is a controlled form of cell death that can lead to various morphological changes, such as nuclear pyknosis, cell surface changes, and chromosome condensation. 25,26 We examined morphological changes by Hoechst 33258 staining and show that A549 cells treated with SCU display specific apoptotic changes in a concentration-dependent manner. Flow cytometry was used to externalize phosphatidylserine (cell surface changes) in A549 cells and our results show that the percentage of apoptotic cells is markedly increased in a concentration-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

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Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Cao

Liu

et al. 2019

Thoracic Cancer

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Background Scutellarin (SCU), a flavonoid isolated from Erigeron breviscapus (Vant.) Hand.‐Mazz., increases autophagy and apoptosis in the adenocarcinoma A549 cell line, which is resistant to cisplatin. However, whether SCU alone has antitumor activity against non‐small cell lung cancer (NSCLC) is unknown. Methods Cell Counting Kit‐8, flow cytometry, colony formation, Hoechst 33258 staining, and Western blot analyses were used to examine the proliferation and apoptosis of A549 cells treated with SCU and the possible molecular mechanisms. Results The cell viability assay indicated that SCU markedly suppressed the proliferation of A549 cells in concentration and time‐dependent manners. SCU caused significant G0/G1 phase arrest and apoptosis, as evidenced by flow cytometric analyses, Hoechst 33258 staining, and Western blot analyses of cyclin D1, cyclin E, BCL‐2, cleaved‐caspase‐3, and BAX. Furthermore, SCU treatment reduced the level of pan‐AKT, phosphorylated (p)‐mTOR, mTOR, BCL‐XL, STAT3, and p‐STAT3, and increased the level of 4EBP1. Conclusions SCU can suppress proliferation and promote apoptosis in A549 cells through AKT/mTOR/4EBP1 and STAT3 pathways. This suggests that SCU may be developed into a promising antitumor agent for treating NSCLC.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Cao

Liu

et al. 2019

Thoracic Cancer

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“…In vivo experiments show that transcriptional regulation of lipogenesis genes (e.g., Srebp-1c, Fas, and Scd) is affected by insulin sensitivity changes in the liver [21]. Several in vivo studies indicate that obesity can be prevented by inhibiting hepatic lipogenesis and suppressing hepatic adipogenesis [22][23][24]. Thus, the preventive effect of kaempferol on obesity-related complications is possibly attributable to the weakened pathological processes of insulin resistance and inflammation [25].…”

Section: Discussionmentioning

confidence: 99%

Preventive Effects of Kaempferol on High-Fat Diet-Induced Obesity Complications in C57BL/6 Mice

Tieqiao

Zhao

2020

BioMed Research International

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Kaempferol is a dietary flavanol that regulates cellular lipid and glucose metabolism. Its mechanism of action in preventing hepatic steatosis and obesity-related disorders has yet to be clarified. The purpose of this research was to examine kaempferol’s antiobesity effects in high-fat diet- (HFD-) fed mice and to investigate its impact on their gut microbiota. Using a completely randomized design, 30 mice were equally assigned to a control group, receiving a low-fat diet, an HFD group, receiving a high-fat diet, and an HFD+kaempferol group, receiving a high-fat diet and kaempferol doses of 200 mg/kg in the diet. After eight weeks, the HFD mice displayed substantial body and liver weight gain and high blood glucose and serum cholesterol levels. However, treatment with kaempferol moderated body and liver weight gain and elevation of blood glucose and serum cholesterol and triglyceride levels. Examination of 16S ribosomal RNA showed that HFD mice exhibited decreased microbial diversity, but kaempferol treatment maintained it to nearly the same levels as those in the control group. In conclusion, kaempferol can protect against obesity and insulin resistance in mice on a high-fat diet, partly through regulating their gut microbiota and moderating the decrease in insulin resistance.

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“…The overexpression of acyl-CoA oxidase (ACO) and carnitine palmitoyltransferase-1 (CPT-1) may be involved in the hepatoprotective effects by regulating mitochondrial fatty acid β-oxidation [ 11 ]. Additionally, gypenosides isolated from G. pentaphyllum showed inhibitory effects on lung cancer cell growth [ 12 ] and improved diabetic cardiomyopathy [ 13 ]. A phytochemical and pharmacological study reported that kaempferol isolated from a water extract of N. nucifera leaves reduced the expression of different adipogenic transcription factors and their target genes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Preventive Effect of Flavonol Derivatives Abundant Sanglan Tea on Long-Term High-Fat-Diet-Induced Obesity Complications in C57BL/6 Mice

Guruvaiah

Guo

et al. 2018

Nutrients

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Sanglan Tea (SLT) is a Chinese medicine-based formulation that is consumed as a health drink for the effective management of obesity-associated complications. However, its chemical components and mechanism of action in the prevention of hepatic steatosis and obesity-related impairments have been uncertain. In this study, we aimed to unveil the chemical profile of SLT and to explore its preventive mechanism in high-fat-diet-induced non-alcoholic fatty liver disease (NAFLD) and obesity-related consequences in C57BL/6 mice. Ultrahigh-performance liquid chromatography (UHPLC) coupled to a quadrupole-orbitrap high-resolution mass spectrometry (MS) analysis of SLT indicated that analogs of quercetin and kaempferol are major compounds of flavonoids in SLT. A dietary supplement of SLT efficiently managed the blood glucose elevation, retained the serum total cholesterol (TC), LDL-cholesterol (LDL-C), and triglyceride (TG) levels, as well as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, and reduced the fat storage in the liver induced by a high-fat diet. The underlying mechanism of this preventive effect is hypothesized to be related to the inhibition of over-expression of lipogenesis and adipogenesis-related genes. Overall, this study suggests that SLT, being rich in quercetin and kaempferol analogs, could be a potential food supplement for the prevention of high-fat-diet-induced NAFLD and obesity-related complications.

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The inhibitory effect of gypenoside stereoisomers, gypenoside L and gypenoside LI, isolated from Gynostemma pentaphyllum on the growth of human lung cancer A549 cells

Cited by 24 publications

References 30 publications

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Preventive Effects of Kaempferol on High-Fat Diet-Induced Obesity Complications in C57BL/6 Mice

Preventive Effect of Flavonol Derivatives Abundant Sanglan Tea on Long-Term High-Fat-Diet-Induced Obesity Complications in C57BL/6 Mice

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