The Inhibitory Effect of<i>Prunella vulgaris</i>L. on Aldose Reductase and Protein Glycation

Li, Hong Mei; Kim, Jin Kyu; Jang, Jai Man; Kwon, Sang Oh; Cui, Cheng Bi; Lim, Soon Sung

doi:10.1155/2012/928159

Cited by 11 publications

(11 citation statements)

References 31 publications

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“…Each 1 mL cuvette contained 531 μ L of 100 mM sodium phosphate buffer (pH 6.2), 90 μ L of AR homogenate, 90 μ L of 1.6 mM NADPH, 9 μ L of the samples dissolved in DMSO, and 90 μ L of 25 mM of DL-glyceraldehyde as the substrate. The RLAR activity was assayed spectrophotometrically by measuring the decrease in the absorption of NADPH at 340 nm over a 4 min period [2]. …”

Section: Methodsmentioning

confidence: 99%

Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant inMaackia amurensis

Kim

Hwang

Wang

et al. 2017

BioMed Research International

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Oxidative stress and aldose reductase activity have been implicated in the development of diabetic complications. In this study, the antioxidant and aldose reductase (AR) inhibitory effects of Maackia amurensis (MA) were investigated. The ethyl acetate fraction of the MA extract showed the highest inhibitory activity in antioxidant and rat lens AR (RLAR). To identify and isolate the active components in the ethyl acetate fraction of the MA extract, high-speed countercurrent chromatography and Sephadex LH-20 column chromatography were performed and guided by an offline HPLC-ABTS assay and HPLC microfractionation AR assay. Four antioxidants, namely, piceatannol (IC50 = 6.73 μM), resveratrol (IC50 = 11.05 μM), trans-ferulic acid (IC50 = 13.51 μM), and chlorogenic acid (IC50 = 27.23 μM), and six AR inhibitors, namely, chlorogenic acid (IC50 = 4.2 μM), tectoridin (IC50 = 50.4 μM), genistein (IC50 = 57.1 μM), formononetin (IC50 = 69.2 μM), resveratrol (IC50 = 117.6 μM), and daidzein (IC50 = 151.9 μM), were isolated and identified. The screening results of the offline HPLC-ABTS assay and HPLC microfractionation AR assay matched the activity of isolated compounds. Thus, MA is potentially valuable for antioxidant and AR inhibitor discovery and efficient drug design for the prevention and treatment of diabetic complications.

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Section: Methodsmentioning

confidence: 99%

Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant inMaackia amurensis

Kim

Hwang

Wang

et al. 2017

BioMed Research International

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“…Furthermore, caffeic acid ethyl ester ameliorates diabetic retinopathy via an anti-oxidant, anti-AGE formation mechanism, and has been shown to possess stronger activity than the commonly-prescribed drug aminoguanidine. These findings suggest that Prunella vulgaris is a potential source of active components capable of ameliorating DR [ 61 ]. However, as is the case with many other medicinal plants, additional studies using animal models are needed to unveil the mechanisms underlying its anti-AGE formation activities.…”

Section: Resultsmentioning

confidence: 99%

Phytochemicals: Target-Based Therapeutic Strategies for Diabetic Retinopathy

Parveen

Kim

et al. 2018

Molecules

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Background: A variety of causative factors are involved in the initiation of diabetic retinopathy (DR). Current antidiabetic therapies are expensive and not easily accessible by the public. Furthermore, the use of multiple synthetic drugs leads to severe side effects, which worsen the diabetic patient’s condition. Medicinal plants and their derived phytochemicals are considered safe and effective treatment and their consumption can reduce the DR risk. In this article, we discuss a variety of medicinal plants, and their noteworthy bio-active constituents, that will be utilized as target based therapeutic strategies for DR. Methods: A broad-spectrum study was conducted using published English works in various electronic databases including Science Direct, PubMed, Scopus, and Google Scholar. Results: Targeting the multiple pathological factors including ROS, AGEs formation, hexosamine flux, PARP, PKC, and MAPK activation through variety of bioactive constituents in medicinal plants, diabetes progression can be delayed with improved loss of vision. Conclusions: Data reveals that traditional herbs and their prominent bioactive components control and normalize pathological cellular factors involved in DR progression. Therefore, studies should be carried out to explore the protective retinopathy effects of medicinal plants using experimental animal and humans models.

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“…Comparable results were observed for RHAR (IC 50 = 55 to 210 µM) and AGE activities (IC 50 = 7.6 µM) [ 66 , 67 , 68 ]. Interestingly, no inhibitory effects were observed for caffeic acid isolated from Perilla frutescens L. [ 69 ] and Prunella vulgaris L. [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

Cataract Preventive Role of Isolated Phytoconstituents: Findings from a Decade of Research

Lim

Schneider

et al. 2018

Nutrients

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Cataract is an eye disease with clouding of the eye lens leading to disrupted vision, which often develops slowly and causes blurriness of the eyesight. Although the restoration of the vision in people with cataract is conducted through surgery, the costs and risks remain an issue. Botanical drugs have been evaluated for their potential efficacies in reducing cataract formation decades ago and major active phytoconstituents were isolated from the plant extracts. The aim of this review is to find effective phytoconstituents in cataract treatments in vitro, ex vivo, and in vivo. A literature search was synthesized from the databases of Pubmed, Science Direct, Google Scholar, Web of Science, and Scopus using different combinations of keywords. Selection of all manuscripts were based on inclusion and exclusion criteria together with analysis of publication year, plant species, isolated phytoconstituents, and evaluated cataract activities. Scientists have focused their attention not only for anti-cataract activity in vitro, but also in ex vivo and in vivo from the review of active phytoconstituents in medicinal plants. In our present review, we identified 58 active phytoconstituents with strong anti-cataract effects at in vitro and ex vivo with lack of in vivo studies. Considering the benefits of anti-cataract activities require critical evaluation, more in vivo and clinical trials need to be conducted to increase our understanding on the possible mechanisms of action and the therapeutic effects.

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The Inhibitory Effect ofPrunella vulgarisL. on Aldose Reductase and Protein Glycation

Cited by 11 publications

References 31 publications

Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant inMaackia amurensis

Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant inMaackia amurensis

Phytochemicals: Target-Based Therapeutic Strategies for Diabetic Retinopathy

Cataract Preventive Role of Isolated Phytoconstituents: Findings from a Decade of Research

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