The inhibitory effect of micafungin on biofilm formation by<i>Pseudomonas aeruginosa</i>

Bazzi, Wael; Sabra, Ahmad; Zahreddine, L T; Khairallah, Marie Therese; Baroud, Maysa; Hadi, Usamah; Matar, Ghassan M.

doi:10.1080/08927014.2013.816299

Cited by 9 publications

(18 citation statements)

References 40 publications

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“…Similar results have been reported by Bazzi et al (2013) and additional research in this area may be helpful in further exploring this treatment option.…”

Section: Discussionsupporting

confidence: 89%

“…Micafungin, by inhibiting the synthesis of 1,3-β-D-glucan, which is present in fungal cell walls and also in P. aeruginosa, may be one of these anti-biofilm agents. Previously, Bazzi et al (2013) showed that micafungin can disrupt the biofilm structure of P. aeruginosa, thereby exposing the core cells to treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have revealed that the ndvB gene encodes for the glucosyltransferase enzyme, which is necessary for the development of 1,3-β-D-glucan in P. aeruginosa (Mennink-Kersten et al 2008;Coulon et al 2010;Beaudoin et al 2012). Previously, Bazzi et al (2013) showed that micafungin can disrupt the biofilm structure of P. aeruginosa, thus possibly exposing the core cells to treatment. Therefore, this study was undertaken to assess the effect of micafungin in combination with each of the four antibacterial agents in vitro and in vivo.…”

Section: Rna Extractionmentioning

confidence: 97%

“…The development of biofilms of P. aeruginosa after the addition of micafungin (10 mg ml −1 ) and MIC doses of the four antibacterial agents was monitored for four days using the protocol described by Bazzi et al (2013) with minor modifications. Briefly, 100 μl of the PAN 14 isolate suspension were transferred in triplicate into a 96-well polystyrene microtiter plate (Costar 3788, Corning Incorporated, Corning, NY, USA).…”

Section: Introductionmentioning

confidence: 99%

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The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

et al. 2016

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Micafungin inhibits biofilm formation by impeding 1,3-β-D-glucan synthesis in Candida albicans. Since Pseudomonas aeruginosa also has 1,3-β-D-glucan in its cell wall, this study assessed the effects of antibacterial agents in vitro and in vivo on micafungin-treated biofilm-forming P. aeruginosa isolates. After treatment with micafungin as well as with a panel of four antibacterial agents, biofilm production was significantly reduced as measured by spectrophotometry. The relative mRNA transcription levels for the genes encoding pellicles (pelC) and cell wall 1,3-β-D-glucan (ndvB), which were measured by quantitative reverse transcription PCR (qRT-PCR), significantly decreased with micafungin treatment. In vivo, the survival rates of P. aeruginosa-infected BALB/c mice significantly increased after combined treatment with micafungin and each of the antibacterial agents. Of these treatments, the combination of micafungin with levofloxacin had the highest survival rate; this combination was the most effective treatment against P. aeruginosa-induced infection.

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“…Similar results have been reported by Bazzi et al (2013) and additional research in this area may be helpful in further exploring this treatment option.…”

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Rna Extractionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

et al. 2016

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“…The plate was incubated for 20 hours at 37ºC then the wells were washed three times with sterile distilled water. Afterwards, subsequent steps of staining with Crystal Violet solution and dissolving in ethanol were done according to a previous protocol [17]. Absorbance was measured with BIO-TEK ELx800 Automated Microplate Reader at 630nm.…”

Section: Assessment Of Biofilm Formation Using the Microtiter Plate Amentioning

confidence: 99%

Genotypic and virulence characteristics of Listeria monocytogenes recovered from food items in Lebanon

Haidar-Ahmad

Kissoyan

Fadlallah

et al. 2016

J Infect Dev Ctries

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Introduction: Listeria monocytogenes is the agent of listeriosis, a life threatening foodborne disease for immunocompromised patients and pregnant women. This bacterium is not routinely screened for in Lebanon and there is lack of data about the prevalent strains and their potential pathogenicity. To that purpose, this study was undertaken to characterize L. monocytogenes from various food products, by assessing the in vitro biofilm forming ability, detecting their virulence potential, and characterizing them at the strain level. Methodology: Fifty-nine isolates were obtained from the Lebanese Agriculture Research Institute (LARI). They were collected in 2012-2013 from local and imported food products in the Lebanese market. Biofilm formation was measured using the Microtiter Plate Assay. PCR amplification was performed for three main virulence genes; hly, actA, and inlB. Pulsed field gel electrophoresis (PFGE) and BIONUMERICS analysis were carried out. Results: Lebanese isolates from cheese and raw meat showed higher biofilm formation than imported and Lebanese seafood isolates. A total of 100% of the isolates were PCR positive for hly and actA genes and 98.3% for inlB gene. PFGE analysis demonstrated the prevalence of 13 different subtypes with 100% similarity. Detected subtypes were grouped into 6 clusters of 90% genomic similarity. Clustered subtypes were particular to the country of origin. Conclusion: This study highlights the presence of L. monocytogenes in the Lebanese food market with high pathogenic potential and stresses the importance of enhanced surveillance and the implementation of strict regulations on local and imported food. Future investigations may be conducted on a larger food selection.

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Effect of 2, 4-di-tert-butylphenol on growth and biofilm formation by an opportunistic fungusCandida albicans

et al. 2015

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Candida albicans, an opportunistic pathogen, has been known to form hypoxic biofilms on medical devices which in turn confers resistance towards antifungals, resulting in subsequent therapeutic failures. Inclusion of anti-biofilm agents in the control of infections is a topic of current interest in developing potential anti-infectives. The in vitro anti-fungal and anti-biofilm efficacy of 2,4-di-tert-butyl phenol [DTBP] was evaluated in this study, which revealed the potential fungicidal action of DTBP at higher concentrations where fluconazole failed to act completely. DTBP also inhibited the production of hemolysins, phospholipases and secreted aspartyl proteinase which are the crucial virulence factors required for the invasion of C. albicans. Various anti-biofilm assays and morphological observations revealed the efficacy of DTBP in both inhibiting and disrupting biofilms of C. albicans. Inhibition of hyphal development, a key process that aids in initial adhesion of C. albicans, was observed, and this could be a mechanism for the anti-biofilm activity of DTBP.

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The inhibitory effect of micafungin on biofilm formation byPseudomonas aeruginosa

Cited by 9 publications

References 40 publications

The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

Genotypic and virulence characteristics of Listeria monocytogenes recovered from food items in Lebanon

Effect of 2, 4-di-tert-butylphenol on growth and biofilm formation by an opportunistic fungusCandida albicans

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