The Inhibitory Effect of Tartary Buckwheat Extracts on Adipogenesis and Inflammatory Response

Lee, Mak-Soon; Shin, Yoonjin; Jung, Sunyoon; Kim, Seog Young; Jo, Young Hee; Kim, Chong Tai; Yun, Min Kyu; Lee, Sung‐Jin; Sohn, Jungwoo; Yu, Heui Jong; Kim, Yangha

doi:10.3390/molecules22071160

Cited by 29 publications

(27 citation statements)

References 65 publications

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“…Hypertrophic adipocyte and immune cell interaction have been well proven for the progression of insulin resistance and noninsulin dependent diabetes [32]. The appropriate ratio of omega-6/omega-3 fatty acid is an important health determinant and decreases the inflammatory condition and cardiovascular disease [33,34]. Our present observation confirmed that the chia seed fatty acid ratio suppresses adipocyte lipogenesis and hypertrophy, which favor the suppression of proinflammatory cytokine formation in the adipose tissue.…”

Section: Resultssupporting

confidence: 82%

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

Subash-Babu

Almaiman

Al-Harbi

et al. 2020

Foods

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The present study aimed to determine the role of Salvia hispanica L., (chia seed) fatty acid content in adipocyte lipid accumulation and human macrophage immunoregulatory potential. Chia seed fatty acid was extracted using hexane by the cold percolation method. A gas chromatography-mass spectrometry (GC-MS) analysis showed a 3:1 ratio of omega 3 and omega 6 fatty acid composition and it was more beneficial for human health. We treated it with increasing concentrations (0–6.4 μg/mL) of chia seed fatty acid extract to determine the cytotoxicity on the preadipocytes and macrophage; no significant cytotoxicity was observed. Chia seed, in 0.2 and 0.4 μg/mL doses, significantly arrested adipocyte hypertrophy and macrophage foam cell development. The gene expression levels of adipocyte confirmed the increased expression of adipocyte mitochondrial thermogenesis related genes, such as uncoupling protein-1 (UCP-1), peroxisome proliferator activated receptor gamma coactivator 1 alpha (PPARγC1α) and PR domain containing 16 (PRDM16); and the down regulated expression of the lipid synthesis related gene sterol regulatory element binding of protein-1c (SREBP-1c). In addition, adipogenesis related genes, such as the proliferator activated receptor γ (PPARγ) and CCAAT/enhancer binding protein (C/EBPα) expressions, have been down regulated by chia seed treatment. Macrophage treated with chia seed-treated adipocyte condition media significantly inhibited the obesity associated inflammatory genes and protein expression levels, such as monocyte chemo attractant protein-1 (MCP-1), prostaglandins E2, interleukin-6, plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-α (TNF-α). In conclusion, a 3:1 ratio of omega 3 and omega 6 fatty acid composition of chia seed fatty acid content potentially inhibits lipid accumulation, and enhanced fatty acid oxidation, via UCP-1 and PRDM16 expression. Macrophage recruitment to adipocyte and the development of obesity associated inflammation was suppressed by chia seeds.

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Section: Resultssupporting

confidence: 82%

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

Subash-Babu

Almaiman

Al-Harbi

et al. 2020

Foods

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“…Researchers found that buckwheat extracts may inhibit adipogenesis and inflammatory response during adipocyte differentiation of 3T3-L1 cells. 24 Brazilian red propolis (Apis mellifera), Copaifera oleoresins, flavonoid fraction of bergamot juice (Citrus bergamia), effusanin C (Isodon japonicus), oligomeric proanthocyanidins (Crataegus oxyacantha) are other isolated compounds with anti-inflammatory actions in activated monocytes and macrophages. [25][26][27][28][29] Lupinus angustifolius L. is a legume that contents a high level of plant protein.…”

Section: Discussionmentioning

confidence: 99%

GPETAFLR, an octapeptide isolated from Lupinus angustifolius L. protein hydrolysate, promotes the skewing to the M2 phenotype in human primary monocytes

et al. 2019

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“…The culture of 3T3-L1 cells was performed as described previously [ 37 ]. Mouse 3T3-L1 preadipocytes were maintained in DMEM containing 10% ( v / v ) BCS, 100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM glutamine under conditions of 37 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxicity of ISOR in adipocytes was evaluated using the WST-8 [2-(2-methoxy-4-nitropheyl)-3-(4-nitrophenyl)-5-(2,4-dinitrophenyl)-2H-tetrazolium, monosodium salt method, with a commercial CCK-8 kit as described previously [ 37 ]. Differentiated 3T3-L1 adipocytes were incubated for 1, 2, 5, or 7 days with ISOR (at a dose of 0, 0.1, 0.5, 1, 10, 20, or 50 µM).…”

Section: Methodsmentioning

confidence: 99%

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Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation

Lee

Kim

2018

Molecules

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Isorhamnetin (ISOR), 3-O-methylquercetin, is a naturally occurring flavonoid in many plants. It is a metabolite derived from quercetin and is known to exert beneficial effects on the prevention of obesity. However, the molecular mechanism of action involved in ISOR-mediated mitochondrial biogenesis, and AMP-activated protein kinase (AMPK) activation in 3T3-L1 cells remains unclear. The aim of this study was to determine whether ISOR affected mitochondrial biogenesis and AMPK activation, during 3T3-L1 adipocyte differentiation. Intracellular lipid and triglyceride accumulation, and glycerol-3-phosphate dehydrogenase (GPDH) activity decreased in ISOR-treated cells. The mRNA levels of adipogenic genes, such as the proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2), were inhibited by ISOR. In contrast, mRNA levels of mitochondrial genes, such as peroxisome proliferator-activated reporter gamma coactivator-1α (PGC-1α), nuclear respiratory factor (NRF)-1, transcription factor A (Tfam), and carnitine palmitoyl transferase-1α (CPT-1α), were all stimulated by ISOR treatment. Mitochondria DNA (mtDNA) copy number and AMPK activity were also stimulated by ISOR. The results suggested that the mitochondrial biogenic effect of ISOR in adipocytes might have been associated with stimulation of mitochondrial gene expression, mtDNA replication, and AMPK activation.

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The Inhibitory Effect of Tartary Buckwheat Extracts on Adipogenesis and Inflammatory Response

Cited by 29 publications

References 65 publications

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

GPETAFLR, an octapeptide isolated from Lupinus angustifolius L. protein hydrolysate, promotes the skewing to the M2 phenotype in human primary monocytes

Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation

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