The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats

Objective: The aim of this study was to develop and optimize the formulation of tablets containing Aquilaria crassna extract using the direct compression method.Methods: D-optimal design based on three independent variables was applied to evaluate the cause-effect relations and optimize the A. crassna tablet formulation. The weight variation (Y1), disintegration time (Y2), hardness (Y3) and friability (Y4) were investigated with respect to three independent variables including % dicalcium phosphate anhydrous (DCPA) in filler (X1), % filler (X2) and % croscarmellose sodium (CCNa) (X3). The dissolution study of the optimized A. crassna tablets were investigated in simulated gastric fluid (SGF) (pH 1.2) using a validated high-performance liquid chromatography (HPLC) method for mangiferin analysis.Results: All investigation factors were found to have significant effects on the physical properties of A. crassna tablet. The tablet hardness and the disintegration time increased in positive relations with the ratios of DCPA. The results exhibited the negative relations between disintegration time and the percentages of CCNa. The optimized A. crassna tablet formulation which included 35 % (w/w) DCPA in filler, 60 % (w/w) filler and 7% (w/w) CCNa possessed the weight variation of 1.38 % (w/w), the disintegration time of 6.29 min, the hardness of 85.63 N and the friability of 0.41 % (w/w). The optimized A. crassna tablet formulation was experimentally examined which demonstrated a good agreement between the experimental and predicted values. Mangiferin was found to release completely from the optimized A. crassna tablets within 30 min. Conclusion:The cause-effect relations and optimization of A. crassna tablet formulation were investigated and reported for the first time. The A. crassna spray-dried extract could be formulated into tablet by direct compression method with good mechanical properties and acceptable release profile.

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“…-Test groups: Y1 [9,13], Y2 [6,12], Y3 [5,7], Y4 [2,13] -Transfer function: Back Propagation Learning…”

Section: X1 (% Dcpa In Filler); X2 (% Filler); X3 (% Ccna); Y1 (Weighmentioning

confidence: 99%

“…1) is one of the active xanthone components of A. crassna leaves [3,4]. Mangiferin has been reported to exhibit antioxidant, anti-diabetic, anti-hyperuricemic, antiviral, anticancer and anti-inflammatory activities [5,6].…”

Section: Introductionmentioning

confidence: 99%

Study on Cause-Effect Relations and Optimization of Tablets Containing Aquilaria Crassna Spray-Dried Extract

Huong

Monsatta

Hanh

et al. 2017

Int J Pharm Pharm Sci

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“…Mangiferin is a flavonoid, extracted from the mango tree; and studies have revealed that it is capable of inhibiting the growth of liver and colon cancer cells, K562 leukemia cells and other tumor cells (11)(12)(13)(14). Pharmacological experiments indicate that mangiferin is capable of effectively treating chronic bronchitis (3).…”

Section: Introductionmentioning

confidence: 99%

“…Casanova-Salas et al (9) demonstrated that miR-182 was a potential biomarker for prostate cancer diagnosis, prognosis predictions and for improvement of the predictive capability of existing biomarkers. Siva et al (10) reported that molecular assays may be used to detect miR-182 expression in prostate cancer.Mangiferin is a flavonoid, extracted from the mango tree; and studies have revealed that it is capable of inhibiting the growth of liver and colon cancer cells, K562 leukemia cells and other tumor cells (11)(12)(13)(14). Pharmacological experiments indicate that mangiferin is capable of effectively treating chronic bronchitis (3).…”

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confidence: 99%

Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182

Li¹,

Ma²,

Yang³

2015

Oncology Letters

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Abstract. Mangiferin, a flavonoid extracted from the mango tree, possesses anti-inflammatory, antibacterial, anti-herpes simplex and antitumor activity, and is able to affect immune function. The present study investigated the anticancer effects of mangiferin treatment on PC3 human prostate cancer cells, and the potential underlying mechanisms. In the present study, an MTT assay was used to analyze the proliferation of PC3 cells. Subsequently, flow cytometry and colorimetric assay kits were utilized to measure the PC3 cell apoptotic rate. The expression levels of B-cell lymphoma-2 (Bcl-2) and microRNA-182 (miR-182) were detected using western blot analysis and quantitative reverse transcription-polymerase chain reaction, respectively. Finally, miR-182 and anti-miR-182 were transfected into PC3 cells, which were used to investigate the effects of mangiferin. Mangiferin treatment reduced the proliferation of PC3 human prostate cancer cells in a concentration-and time-dependent manner. In addition, mangiferin was able to promote apoptosis and induce the caspase-3 activity of PC3 human prostate cancer cells. Mangiferin treatment was also able to significantly reduce Bcl-2 expression levels and enhance miR-182 expression in PC3 cells. Finally, it was observed that mangiferin inhibited proliferation and induced apoptosis in PC3 human prostate cancer cells, and this effect was correlated with downregulation of Bcl-2 and upregulation of miR-182. IntroductionProstate cancer is a disease that affects older males, and is one of the most common malignant tumors amongst males in Europe (1). Previously, prostate cancer was observed relatively rarely in China; however, in recent years the incidence of prostate cancer and associated mortality rates have been increasing (1).In 2008, the worldwide standardized incidence rate of prostate cancer was 28.5/100,000 and the standardized mortality rate was 7.5/100,000 (2-4). Therefore, prostate cancer has become a significant area of research.Cell apoptosis may be inseparable from the development of prostate cancer. Apoptosis, or programmed cell death, differs from necrosis, the natural cell death process (5). Reduced apoptosis and increased proliferation are thought to be the primary mechanisms underlying the formation of the majority of tumors. Inhibition of apoptosis may also possess a significant role in the progression of prostate cancer, and B-cell lymphoma-2 (Bcl-2) is thought to be one of the most significant genes required for apoptosis (6).MicroRNAs (miRs) are a class of endogenously expressed non-protein encoding RNAs (2). Mature miRs are single-stranded and consist of ~22 nucleotides (7). Previous studies have indicated that miRs may be involved in human tumorigenesis and development. Numerous tumors exhibit alterations in miR expression levels, and these miRs may have a role in tumorigenesis, functioning as cancer genes or tumor suppressor genes (8). Casanova-Salas et al (9) demonstrated that miR-182 was a potential biomarker for prostate cancer diagnosis, prognosis predict...

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“…It is known that mangiferin (I) (Figure 1) is potentially a naturally-occurring chemopreventive agent in rat colon carcinogenesis (Yoshimi et al, 2001), exerts antidiabetic activity by increasing insulin sensitivity (Ichiki et al, 1998;Sellamuthu et al, 2009), appears to act as a potential biological response modifier with antitumor, immune modulatory and anti-HIV effect (Guha et al, 1996), is useful as an analgesic without adverse effects (Makare et al, 2001), and inhibits the late event in Herpes Simplex/Virus-2 replication (Zhu et al, 1995). A thin layer chromatography (TLC) densitometric method was developed for the quantitative determination of I in leaves of Cratoxylum pruniflorum (Nedialkov et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Determination of mangiferin in Mangifera indica L. stem bark extract (Vimang®) and pharmaceuticals by liquid chromatography

Gil

Hernández²,

Paz

et al. 2014

Emir. J. Food Agric

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A new liquid chromatography method for the analysis of mangiferin (I) from Mangifera indica L. stem bark extract (Vimang®), and pharmaceuticals is described. Screening experiments were performed by an experimental design to find the influence of some important chromatographic variables (methanol, column temperature and acetic acid) on the retention times and resolution between critical pairs in the separation. This design also permitted to estimate method robustness and optimal conditions to achieve the best resolution. The best separation was found using a LiChrospher RP 18, 5 µm, 250 x 4.6 mm I.D. column maintained at 25°C, a mobile phase comprising methanol-2.5% v/v aqueous solution of acetic acid (280:720, v/v) at a flow rate of 1.0 ml/min, and detection by UV at 254 nm. The method resolves I, the major component, from other components of the extract. The method showed good selectivity, repeatability (RSD < 2%) and linearity (r = 0.9998). The limits of detection and quantitation were 0.008% (0.9 ng) and 0.05% (6.2 ng), respectively, relative to a 0.6 mg/ml standard solution, injection volume 20 µl. This method was used to quantify I in some aqueous extracts from the natural product and pharmaceuticals.

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The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats

Cited by 199 publications

References 18 publications

Study on Cause-Effect Relations and Optimization of Tablets Containing Aquilaria Crassna Spray-Dried Extract

Study on Cause-Effect Relations and Optimization of Tablets Containing Aquilaria Crassna Spray-Dried Extract

Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182

Determination of mangiferin in Mangifera indica L. stem bark extract (Vimang®) and pharmaceuticals by liquid chromatography

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