1989
DOI: 10.1016/0192-0561(89)90174-4
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The inhibitory effects of N-ethylmaleimide, colchicine and cytochalasins on human T-cell functions

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Cited by 26 publications
(18 citation statements)
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“…Cytochalasin A is chemically similar to the thiol-alkylating agent N-ethylmaleimide (NEM) and has secondary effects on cell metabolism and thiol alkylation in mammalian cells that are unrelated to effects on actin (18,32,35). In this study, unlike studies with mammalian cells in which NEM did not interfere with actin filaments (18), NEM did in fact interfere with actin polymerization in C. albicans (not shown).…”
Section: Discussioncontrasting
confidence: 43%
See 1 more Smart Citation
“…Cytochalasin A is chemically similar to the thiol-alkylating agent N-ethylmaleimide (NEM) and has secondary effects on cell metabolism and thiol alkylation in mammalian cells that are unrelated to effects on actin (18,32,35). In this study, unlike studies with mammalian cells in which NEM did not interfere with actin filaments (18), NEM did in fact interfere with actin polymerization in C. albicans (not shown).…”
Section: Discussioncontrasting
confidence: 43%
“…Cytochalasin A is chemically similar to the thiol-alkylating agent N-ethylmaleimide (NEM) and has secondary effects on cell metabolism and thiol alkylation in mammalian cells that are unrelated to effects on actin (18,32,35). In this study, unlike studies with mammalian cells in which NEM did not interfere with actin filaments (18), NEM did in fact interfere with actin polymerization in C. albicans (not shown). The differential effect of NEM on actin from C. albicans compared to mammalian cells points to an intrinsic difference in sensitivity to oxidation between the two actins in their respective environments that may reflect the observation by us and others (32) that yeast and fungi are sensitive to cytochalasin A but not to cytochalasin B.…”
Section: Discussioncontrasting
confidence: 43%
“…Note, however, that F-actin binding of a second costimulation-related actin-binding protein, L-Plastin (30), is not reduced by CPH (data not shown). Moreover, the effects of CPH clearly differ from those of cytochalasins, well accepted inhibitors of actin polymerization (40,41). Thus, although in parallel experiments cytochalasins inhibited T cell proliferation, they did not reduce IL-2 production (data not shown).…”
Section: Discussionmentioning
confidence: 82%
“…Cytochalasin B depolymerizes microfilaments, a process which has a number of effects that inhibit antigen uptake and processing (14,17,54). However, low concentrations of cytochalasin B enhance T-cell responses to mitogens such as ConA (17,24,47,51).…”
Section: Resultsmentioning
confidence: 99%