The Inhibitory Effects of Pure Black Tea Theaflavins on the Growth of Four Selected Human Cancer Cells

Abstract

The growth inhibition and cytotoxic effects of theaflavins mixture and pure theaflavins [theaflavin, theaflavins-3-gallate, theaflavins-3′-gallate and theaflavin-3, 3′-digallate] from black tea on human liver cancer HepG2 cells, gastric cancer SGC-7901 cells, lung cancer A549 cells and acute promyelocytic leukemia K562 cells were investigated. The four major theaflavins were obtained from enzymatically oxidized catechins mixture using preparative high-performance liquid chromatography (HPLC). The purity and the structures of the isolated compounds were confirmed by Journal of Food Biochemistry ISSN 1745-4514

Synergistic effect of black tea polyphenol, theaflavin-3,3′-digallate with cisplatin against cisplatin resistant human ovarian cancer cells

“…It’s orange-red in color and possesses a benzotropolone skeleton that is formed from the co-oxidation of (–)-epicatechin gallate and (–)-epigallocatechin gallate (EGCG) during black tea production (Finger, 1994). TF3 has been demonstrated to inhibit human prostate cancer cells (Lee, Ho, & Lin, 2004; Sun, et al, 2013), liver cancer cells, gastric cancer cells and lung cancer cells (K. Wang, et al, 2011). TF3 exerted antitumor effects in breast cancer cells through suppressing proteasomal activities (Lin, Chen, & Lin-Shiau, 2006).…”

Theaflavin-3,3′-Digallate Enhances the Inhibitory Effect of Cisplatin by Regulating the Copper Transporter 1 and Glutathione in Human Ovarian Cancer Cells

“…TF3 is a polyphenolic compound extracted from black tea and is formed via the co-oxidation of (−)-epigallocatechin gallate (EGCG) and (−)-epicatechin gallate during black tea production [ 17 ]. TF3 has shown potent antioxidant [ 18 ], anti-inflammatory [ 19 ], and anticancer properties [ 20 , 21 , 22 ]. We have previously reported that TF3 inhibited human ovarian cancer cells by inducing apoptosis and cell cycle arrest [ 23 ] and by suppressing angiogenesis [ 24 ].…”

Tannase-mediated biotransformation assisted separation and purification of theaflavin and epigallocatechin by high speed counter current chromatography and preparative high performance liquid chromatography: A comparative study

“…Generally, it requires organic acids to assist the separation and avoid the overlapping between theaflavins and catechins. Since the frequently chosen organic acids (Wang et al, ; Xu et al, ) are formic acid, acetic acid, phosphate acid, trifluoroacetic acid that are difficult to get rid of. The concentrated theaflavins fractions are always washed repeatedly with water to remove the residual acid.…”