The inhibitory mechanism of &lt;em&gt;Cordyceps sinensis&lt;/em&gt; on cigarette smoke extract-induced senescence in human bronchial epithelial cells

Liu, Ailing; Wu, Jinxiang; Li, Aijun; Bi, Wenxiang; Liu, Tian; Cao, Liuzhao; Liu, Yahui; Dong, Liang

doi:10.2147/copd.s107396

Cited by 28 publications

(17 citation statements)

References 44 publications

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“…LTB4 is a potent neutrophil chemoattractant and activator and a very important regulator of inflammation in vivo [26]. LTB4 can activate and aggregate a variety of inflammatory and immune effector cells [27].…”

Section: Discussionmentioning

confidence: 99%

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

Zhou

et al. 2017

J Cardiothorac Surg

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BackgroundLung transplantation is the only effective treatment for end-stage lung diseases. Bronchiolitis obliterans, which is known as non-infectious chronic lung allograft dysfunction (CLAD) in the new classification, is the greatest threat to long-term survival after lung transplantation. This study investigated the role of leukotriene B4 (LTB4) and montelukast in transplantation-related bronchiolitis obliterans and discussed the pathophysiological significance of LTB4 in chronic rejection.MethodsRats were randomly divided into an experimental group (montelukast), a positive control group (dexamethasone), and a blank control group (normal saline solution; NS). Each piece of trachea removed from a F344 rat was transplanted into a Lewis rat through a 5-mm incision at the episternum by subcutaneous embedding. The recipients were treated with gastric lavage with 3 mg/kg · d montelukast suspension, 1 mg/kg · d dexamethasone, and 1 mL/kg · d NS, respectively, in each group. On Day 28, peripheral blood was drawn to measure the white blood cell counts and plasma LTB4 levels. The donor specimens were stained by H-E and Masson, and their organizational structure and extent of fibrosis were visually assessed. The measurement data were compared using one-way analysis of variance, and the categorical data were compared using the chi-square test. A P value of less than 0.05 was considered to indicate statistical significance.ResultsThe white blood cell counts of the montelukast, dexamethasone, and NS groups were (16.0 ± 4.2) × 109/L, (19.5 ± 11.6) × 109/L, and (25.8 ± 3.6) × 109/L; no statistical significance was found (P = 0.101). The concentrations of LTB4 were 2230 ± 592 pg/mL, 1961 ± 922 pg/mL, and 3764 ± 1169 pg/mL, and statistical significance was found between the NS group and each of the others (P = 0.009). The percentages of tracheal occlusion were 73.6% ± 13.8%, 23.4% ± 3.2%, and 89.9% ± 11.3%, and statistical significance was found among the three groups (P = 0.000).ConclusionsThe study established a model to simulate bronchiolitis obliterans after clinical lung transplantation. Oral administration of montelukast reduced plasma LTB4 levels in rats and played a preventive role against tracheal fibrosis after transplantation. This suggests that LTB4 may be involved in bronchiolitis obliterans after pulmonary transplantation. This study indicates a new direction for research into the prevention and treatment of bronchiolitis obliterans after lung transplantation.

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Section: Discussionmentioning

confidence: 99%

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

Zhou

et al. 2017

J Cardiothorac Surg

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“… 17 – 19 Numerous studies have shown that the C. sinensis extracts have a therapeutic effect on patients with COPD, including improvement of symptoms, quantity of life, and lung function. 20 , 21 A mechanistic study suggests that the C. sinensis extract inhibits the senescence induced by CSE in human bronchial epithelial cells via the ROS/PI3K/AKT/mTOR signaling pathway. 20 …”

Section: Introductionmentioning

confidence: 99%

“… 20 , 21 A mechanistic study suggests that the C. sinensis extract inhibits the senescence induced by CSE in human bronchial epithelial cells via the ROS/PI3K/AKT/mTOR signaling pathway. 20 …”

Section: Introductionmentioning

confidence: 99%

“…Ophiocordyceps sinensis is rich in nucleosides, polysaccharides, sterols, proteins, amino acids, and polypeptides. 20 The nucleosides are recognized as the main bioactive components in C. sinensis , and are used as chemical markers for quality control of C. sinensis . 22 , 23 The objective of this work was to explore the anti-inflammatory effect and preliminary pathways of nucleosides from cultured C. sinensis on RAW264.7 macrophages and COPD mice stimulated by CSE.…”

Section: Introductionmentioning

confidence: 99%

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Nucleosides isolated from <em>Ophiocordyceps sinensis</em> inhibit cigarette smoke extract-induced inflammation via the SIRT1–nuclear factor-κB/p65 pathway in RAW264.7 macrophages and in COPD mice

Sun¹,

Dong²,

Li³

et al. 2018

COPD

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BackgroundOphiocordyceps sinensis (C. sinensis) extracts have been found to have a therapeutic effect on patients with chronic obstructive pulmonary disease (COPD). Silent information regulator 1 (SIRT1) plays an important role in the regulation of inflammatory mediators and correlates with lung function and COPD exacerbations. The objective of this work was to explore the anti-inflammatory effect and preliminary pathways of nucleosides from cultured C. sinensis on RAW264.7 macrophages and COPD mice.Materials and methodsThe nucleosides were extracted from cultured C. sinensis powder and further purified by macroporous resin D101 and glucan G10 columns. Inflammation and oxidative stress models in RAW264.7 macrophages and in mice were established by injection of cigarette smoke extract (CSE). We then examined how the isolated nucleosides regulated the production of the associated inflammatory mediators in vitro and in vivo by enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and Western blot.ResultsThe nucleosides inhibited inflammatory mediator expression of tumor necrosis factor-α, interleukin-6, interleukin-1β, and nitric oxide in both the CSE-stimulated RAW264.7 macrophages and mice. Moreover, the nucleosides elevated SIRT1 activation and suppressed nuclear factor-κB (NF-κB)/p65 activation in vitro and in vivo. Nucleoside treatment significantly decreased the levels of the inflammatory mediators in the bronchoalveolar lavage fluid (BALF) and serum of the CSE-induced mice. The nucleosides also altered the recruitment of inflammatory cells in BALF and improved characteristic features of the lungs in the CSE-induced mice.ConclusionThese results show that the nucleosides suppressed COPD inflammation through the SIRT1–NF-κB/p65 pathway, suggesting that the nucleosides may be partly responsible for the therapeutic effects of cultured C. sinensis on COPD patients.

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“…Previous studies have indicated that this medicine may also have a protective effect against lung diseases ( 23 , 24 ). In a previous study conducted by the present research group, it was revealed that C. sinensis was able to significantly inhibit senescence via the reactive oxygen species and phosphoinositide 3-kinase/AKT/mechanistic target of rapamycin signaling pathways in cigarette smoke extract (CSE)-induced 16 human bronchial epithelial cells (HBEs) ( 25 ). Previous reports have suggested that C. sinensis may have anti-fibrotic effects ( 24 , 26 , 27 ).…”

Section: Introductionmentioning

confidence: 96%

Cordycepsï¿½sinensis inhibits airway remodeling in rats with chronic obstructive pulmonary disease

Yang

Jiao

et al. 2018

Exp Ther Med

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Cordyceps sinensis is a traditional Chinese herbal medicine that has been used for centuries in Asia as a tonic to soothe the lung for the treatment of respiratory diseases. The aim of the present study was to determine the effects of C. sinensis on airway remodeling in chronic obstructive pulmonary disease (COPD) and investigate the underlying molecular mechanisms. Rats with COPD were orally administered C. sinensis at low, moderate or high doses (2.5, 5 or 7.5 g/kg/day, respectively) for 12 weeks. Airway tissue histopathology, lung inflammation and airway remodeling were evaluated. C. sinensis treatment significantly ameliorated airway wall thickening, involving collagen deposition, airway wall fibrosis, smooth muscle hypertrophy and epithelial hyperplasia in model rats with COPD. Additionally, C. sinensis administration in rats with COPD reduced inflammatory cell accumulation and decreased inflammatory cytokine production, including tumor necrosis factor-α, interleukin-8 and transforming growth factor (TGF)-β1 in bronchoalveolar lavage fluid. Meanwhile, the increased levels of α-smooth muscle actin and collagen I in the COPD group were also markedly decreased by C. sinensis treatment. Furthermore, compared with untreated rats with COPD, C. sinensis reduced the expression level of phosphorylated (p)-Smad2, p-Smad3, TGF-β1 and its receptors, with the concomitant increased expression of Smad7 in the lungs of rats with COPD. These results indicated that treatment with C. sinensis may be a useful approach for COPD therapy.

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The inhibitory mechanism of <em>Cordyceps sinensis</em> on cigarette smoke extract-induced senescence in human bronchial epithelial cells

Cited by 28 publications

References 44 publications

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

Nucleosides isolated from <em>Ophiocordyceps sinensis</em> inhibit cigarette smoke extract-induced inflammation via the SIRT1–nuclear factor-κB/p65 pathway in RAW264.7 macrophages and in COPD mice

Cordycepsï¿½sinensis inhibits airway remodeling in rats with chronic obstructive pulmonary disease

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The inhibitory mechanism of <em>Cordyceps sinensis</em> on cigarette smoke extract-induced senescence in human bronchial epithelial cells

Cited by 28 publications

References 44 publications

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

LTB4 and montelukast in transplantation-related bronchiolitis obliterans in rats

Nucleosides isolated from <em>Ophiocordyceps sinensis</em> inhibit cigarette smoke extract-induced inflammation via the SIRT1&ndash;nuclear factor-&kappa;B/p65 pathway in RAW264.7 macrophages and in COPD mice

Cordycepsï¿½sinensis inhibits airway remodeling in rats with chronic obstructive pulmonary disease

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Nucleosides isolated from <em>Ophiocordyceps sinensis</em> inhibit cigarette smoke extract-induced inflammation via the SIRT1–nuclear factor-κB/p65 pathway in RAW264.7 macrophages and in COPD mice