The inhibitory role of effective microorganisms on the growth of pathogenic bacteria

Abstract

This study was conducted to evaluate the efficacy of Effective Microorganisms (EM1 ® ) for inhibiting the growth of some pathogenic bacteria Staphylococcus aureus and E. coli were used in this study and isolated from pathological conditions. These bacteria were diagnosed in laboratory of microbiology, College of Veterinary Medicine, University of Mosul. The colonies that taken from blood agar were 5-7 and cultured in the nutrient broth and incubated at 37 ºC for 24 hours. Bacterial growth was calibrated with the second tube of the McFarland tubes 0.5%. Several concentrations of EM product were prepared 1, 0.5, 0.25 and 0.125%. Decimal dilutions were done for each concentration of EM product with bacterial suspension, except control group was done for bacterial suspension with nutrient broth. The bacterial count was done on nutrient agar, milk agar and EMB agar. The results of this study showed that the product of EM1 ® within concentrations 0.5-1% was highly efficient in inhibiting the growth of pathogenic bacteria under study. The bacterial count of both S. aureus and E. coli was 54x10 7 and 52x10 7 CFU/ ml respectively at 1% EM1 ® , and 67x10 7 and 86x10 7 CFU/ ml respectively at 0.5%, while the counting of the control group was 42x10 9 and 67x10 9 CFU/ ml respectively. This study concluded that EM1 ® at low concentrations have a clear role in inhibiting the growth of pathogenic bacteria, particularly S. aureus and E. coli.

The Impact of Effective Microorganisms (EM) on Egg Quality and Laying Performance of Chickens

“…This might be due to the beneficial effect of the effective microorganisms in the gut of the chickens by suppressing pathogenic bacteria and facilitating digestion and absorption of nutrients. Moreover, others [25] also confirmed that the use of EM at 1% to have the best effect in reducing the growth of pathogenic bacteria. In contrast to the current result, Fathiet al (2018) [26] reported no significant difference in egg production performance among the different dietary treatments.…”

Effective Microorganisms (EM) Improve Internal Organ Morphology, Intestinal Morphometry and Serum Biochemical Activity in Japanese Quails under Clostridium perfringens Challenge

“…Further studies are needed to evaluate EM activity against other pathologic bacteria ( Escherichia coli and Staphylococcus aureus ). Hamad et al [ 36 ] performed microbiological studies with the mentioned bacteria and found an inhibiting role of EM in the growth of pathogenic bacteria. Thus, further studies involving EM in animals are needed, especially in birds, to improve EM as a potential alternative to antibiotics used as growth promoters and to develop future natural production systems.…”

Pathological study of genitourinary invasion by Aspergillus flavus in male rats

“…Therefore, milk may have considered to be a serious source of infection by this fungus. Considered the Aspergilli as one of effective fermenting microorganisms which may use for inhibition of pathogenic bacterial growth (23). In addition, (24) mentioned that Aspergillus species was recorded as one cause of urinary system organs, in particular the urinary bladder in calves.…”