The inhibitory role of Mir-29 in growth of breast cancer cells

Wu, Zhigang; Huang, Xiaona; Huang, Xing; Zou, Qiang; Guo, Yang

doi:10.1186/1756-9966-32-98

Cited by 69 publications

(55 citation statements)

References 30 publications

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“…Similarly, Wei et al (26) recently reported the up-regulation of miR-29 family members with age in postnatal mouse skeletal muscle and during muscle differentiation and showed that miR-29 negatively regulates muscle cell proliferation by targeting the Akt signaling pathway. These findings, combined with numerous reports on miR-29 as a tumor suppressor (27)(28)(29), collectively suggest that miR-29 plays an important role in regulating proliferation of normal cells during postnatal life as well as malignant cells.…”

Section: Discussionmentioning

confidence: 52%

Evidence That Up-Regulation of MicroRNA-29 Contributes to Postnatal Body Growth Deceleration

Kamran

Andrade

Nella

et al. 2015

Molecular Endocrinology

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Body growth is rapid in infancy but subsequently slows and eventually ceases due to a progressive decline in cell proliferation that occurs simultaneously in multiple organs. We previously showed that this decline in proliferation is driven in part by postnatal down-regulation of a large set of growth-promoting genes in multiple organs. We hypothesized that this growth-limiting genetic program is orchestrated by microRNAs (miRNAs). Bioinformatic analysis identified target sequences of the miR-29 family of miRNAs to be overrepresented in age-down-regulated genes. Concomitantly, expression microarray analysis in mouse kidney and lung showed that all members of the miR-29 family, miR-29a, -b, and -c, were strongly up-regulated from 1 to 6 weeks of age. Real-time PCR confirmed that miR-29a, -b, and -c were up-regulated with age in liver, kidney, lung, and heart, and their expression levels were higher in hepatocytes isolated from 5-week-old mice than in hepatocytes from embryonic mouse liver at embryonic day 16.5. We next focused on 3 predicted miR-29 target genes (Igf1, Imp1, and Mest), all of which are growth-promoting. A 3'-untranslated region containing the predicted target sequences from each gene was placed individually in a luciferase reporter construct. Transfection of miR-29 mimics suppressed luciferase gene activity for all 3 genes, and this suppression was diminished by mutating the target sequences, suggesting that these genes are indeed regulated by miR-29. Taken together, the findings suggest that up-regulation of miR-29 during juvenile life drives the down-regulation of multiple growth-promoting genes, thus contributing to physiological slowing and eventual cessation of body growth.

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Section: Discussionmentioning

confidence: 52%

Evidence That Up-Regulation of MicroRNA-29 Contributes to Postnatal Body Growth Deceleration

Kamran

Andrade

Nella

et al. 2015

Molecular Endocrinology

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“…Zhang et al discovered that hsa-miR-21 down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in non-small cell lung cancer [38]. Wu et al reported that hsa-miR-29a, a member of the hsa-miR-29 family, plays an important role in inhibiting growth of breast cancer cells and arresting cells at G0/G1 phase [39]. In addition, several researchers have also studied the possibility of using hsa-miR-21 as biomarker for GC diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Hsa-miR-21 and Hsa-miR-29 in Tissue as Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer

Wang

Zhang

Liu

et al. 2015

Cell Physiol Biochem

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Background/Aims: Gastric cancer (GC) is the fourth most common cancer and the second most common cause of cancer deaths worldwide. Endoscopic examination is the most used method to detect the GC nowadays, whereas this method is expensive and invasive. MicroRNAs (miRNAs) are a group of recently discovered small non-protein-coding RNAs. They regulate the expression of hundreds of target genes; thereby control a wide range of tumorigenic processes. In this study, we selected two miRNAs, hsa-miR-21 and hsa-miR-29, as the targets to assess their diagnostic and prognostic value for GC. Methods: A total of 50 GC patients including 24 females and 26 males were recruited. Tumor and adjacent non-tumor tissue samples were collected from all these participants during the endoscopic examination. RNAs were extracted from these samples, then quantified via qRT-PCR and normalized with RNU43 as the internal control. Statistical analyses were conducted using the GraphPad Prism 5.0. Results: We discovered a higher expression of hsa-miR-21 and a relatively lower expression of hsa-miR-29hsa-miR-29 in the tumor tissue than in the adjacent non-tumor tissue. Moreover, both the two miRNAs showed moderate diagnostic performance (hsa-miR-21: AUC = 0.75, sensitivity = 0.70, specificity = 0.78; hsa-miR-29hsa-miR-29: AUC = 0.73, sensitivity = 0.70, specificity = 0.68). In the follow-up research, we found that higher tissue hsa-miR-21 level was related to a lower overall survival rate, whereas higher tissue hsa-miR-29hsa-miR-29 level was associated with the higher overall survival rate. These results indicated that both hsa-miR-21 and hsa-miR-29 had the potential to be the biomarkers for GC prognosis. Conclusion: In summary, we verified the diagnostic and prognostic value of tissue hsa-miR-21hsa-miR-21 and hsa-miR-29 in GC. Both of them can be potentially applied as novel and non-invasive biomarkers for GC.

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“…cancers, including breast cancer, glioma, and colorectal carcinoma [21][22][23]. However, the relationship between miR-29a expression and CCA prognosis has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of miR-29a and Its Prognostic Significance in Patients with Cholangiocarcinoma

Deng

Chen²

2017

Oncol Res Treat

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Background: This study aimed to examine the expression level and prognostic value of microRNA-29a (miR-29a) in human cholangiocarcinoma (CCA). Patients and Methods: The expression of miR-29a was measured in tissues collected from 125 CCA patients using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Survival analysis was carried out using the Kaplan-Meier method and log-rank test. The prognostic value of miR-29a was examined with Cox regression analysis. Results: The results of qRT-PCR demonstrated that miR-29a expression was significantly upregulated in CCA tissue samples compared with matched noncancerous samples. Overexpression of miR-29a was found to be correlated with lymph node metastasis, clinical stage, and differentiation of CCA. The Kaplan-Meier survival curves suggested that patients with high miR-29a levels had poor overall survival compared to those with low miR-29a expression. From the results of the Cox regression analysis, we considered increased miR-29a expression to be an independent prognostic factor for patients with CCA. Conclusion: Our study data demonstrated that upregulated miR-29a expression is associated with progression of CCA and might act as a prognostic biomarker in CCA patients.

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The inhibitory role of Mir-29 in growth of breast cancer cells

Cited by 69 publications

References 30 publications

Evidence That Up-Regulation of MicroRNA-29 Contributes to Postnatal Body Growth Deceleration

Evidence That Up-Regulation of MicroRNA-29 Contributes to Postnatal Body Growth Deceleration

Hsa-miR-21 and Hsa-miR-29 in Tissue as Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer

Increased Expression of miR-29a and Its Prognostic Significance in Patients with Cholangiocarcinoma

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