The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification

Abstract

The aim of this study was to investigate the influence of hydrophobic acid-etched (A) and coarse-blasted large-grit and acid-etched (SLA) surfaces as well as hydrophilic modified acid-etched (modA) and modified coarse-blasted large-grit and acid-etched (modSLA) surfaces on the behavior of MG63 cells grown on these surfaces through determination of cell attachment and cell proliferation, time-lapse microscopy of fluorescence-labeled cells, and determination of gene expression by reverse transcription-polymerase chain reaction (RT-PCR). No significant difference of cell attachment on various titanium surfaces was found. Increased cell proliferation was observed on the A surface and the SLA surface compared with the modA surface and the modSLA surface. After 2 days of incubation, on modSLA and modA surfaces a tendency of formation of cell clusters has been observed, which was most pronounced on modSLA surface. On the A and the SLA surface, cell cluster formation started after longer incubation periods. The expression level of the bone-associated genes (alkaline phosphatase, osteocalcin, type-I-collagen, osteoprotegerin, and glyceraldehyde-3-phosphate-dehydrogenase) detected by RT-PCR was highest on the modSLA surface. In conclusion it has been demonstrated that the modSLA surface results in an enhanced cluster formation of osteoblasts grown on this surface and in an increased expression of key osteogenic regulatory genes in osteoblasts.

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

“…Implementation of Ti implants with moderately rough surface topography resulted in substantially improved tissue integration of the implants 20) , which is presumably associated with their positive effect on the proliferation and differentiation of osteoblasts 3,21,22) . One of the possible strategies for further development of the implant therapy could be enhancing the angiogenesis.…”

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

“…A previous study showed that superhydrophilicity was maintained by storage in distilled water immediately after sandblasting and acid etching of titanium (Ti) 5) , and this surface enhanced cell response 6) . In addition, more differentiated phenotypes of cells grown on the superhydrophilic surface were confirmed by the remarkable increase of alkaline phosphatase activity, osteocalcin, and osteoprotegerin production [7][8][9] .…”

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

“…To improve the osteoconductive and osteogenic properties for optimal osteointegration, the surface of titanium implants was constantly modified, mostly trying to increase its hydrophilicity 7) . The microstructure and the nanostructure of the titanium surface influenced the behavior of mesenchymal precursors of osteoblasts, thus affecting cell proliferation and differentiation towards the osteoblastic lineage [8][9][10][11] . Mesenchymal stem cells are capable of self-renewal and, when appropriately induced, of differentiating into many cellular lineages [12][13][14] , undifferentiated and highly clonogenic cells may be isolated from the dental pulp, the periodontal ligament and the dental papilla 15,16) .…”