The insensitivity to uncouplers of testis mitochondrial ATPase

Vázquez-Memije, Marta Elisa; Izquierdo-Reyes, Víctor; Delhumeau-Ongay, G.

doi:10.1016/0003-9861(88)90425-0

Cited by 5 publications

(1 citation statement)

References 38 publications

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“…Binding of ATP to BSA0 to our knowledge, has not yet been described. BSA, however, has been utilized in several ATP-dependent systems as ionmotive ATPases, proteases and others, mainly to antagonize drug-induced effects (for examples, see [4][5][6][7][8][9][10]). Schraw and Post described covalent labeling of BSA by the dialdehyde derivative of ATP which was prevented by high concentrations of ATP.…”

Section: Introductionmentioning

confidence: 99%

ATP binding to bovine serum albumin

1992

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Specific binding of ATP to bovi~,e serum albumin (BSA) is demonstrated employing ATP derivatives spin-labeled at either N 6 or C8 of adenine ring or at the ribose moiety. Based on a 1:1 stoichiomctry binding constants are in the 50-100,tiM range. Binding is largely competitive with ATP or ste.'tric acid, A snaall fraction or the I~heled nueleotides could not be liberated by these ligands. Binding of AMP is in the millimolar range, only.

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Section: Introductionmentioning

confidence: 99%

ATP binding to bovine serum albumin

1992

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Analysis of age-associated changes in mitochondrial free radical generation by rat testis

et al. 2007

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Throughout spermatogenesis, mitochondria undergo a morphological and functional differentiation. Mitochondria are involved in the production of reactive oxygen species (ROS), considered one of the mediators of ageing. Particularly, lipid peroxidation is regarded as a major phenomenon by which ROS can impair cellular function. In the present study, we examined the production of superoxide anion, superoxide dismutase activity and the effect of Fe(2+)/ascorbate induced-lipid peroxidation on the respiratory chain activities of testis mitochondria throughout the process of spermatogenesis and ageing. Mitochondria from rat testes generated superoxide anion, mainly using NADH as substrate, which increased according to age. The activity of SOD is age-dependent and greatly stimulated during the first wave of spermatogenesis, but decreases in adulthood and old age. TBARS concentration was also markedly increased by ageing. The activity of mitochondrial respiratory chain complexes is differentially affected by oxidative stress induced by iron/ascorbate, succinate-dehydrogenase activity being less vulnerable than that of NADH-dehydrogenase and cytochrome c oxidase. The data suggest that ageing is accompanied by reduced activity of SOD, leading to excessive oxidative stress and enhanced lipid peroxidation that compromises the functionality of the electron transport chain. The data support the concept that mitochondrial function is an important determinant in ageing.

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