The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins

Wang, Wanhe; Wang, Xueliang; Liu, Jingqi; Lin, Chuankai; Liu, Jianhua; Wang, Jing

doi:10.3390/bios12060421

Cited by 12 publications

(4 citation statements)

References 108 publications

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“…(e) GNPs modulate DNA polymerase activity, mimicking hot start effect. Given their biocompatibility, large surface area, ease of preparation, and the possibility of surface modi cation, GNPs offer a valuable tool for boosting the PCR ampli cation process 39,40 .…”

Section: Discussionmentioning

confidence: 99%

Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses

Ghoniem

ElZorkany²,

Hagag

et al. 2023

Preprint

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Gold nanoparticles (GNPs) biosensors can detect low viral loads and differentiate between viruses types, enabling early diagnosis and effective disease management. In the present study, we developed GNPs biosensors with two different capping agent, citrate- GNPs biosensors and polyvinylpyrrolidone (PVP)- GNPs biosensors for detection of EHV-1 and EHV- 4 in multiplex real time PCR (rPCR). Citrate- GNPs and PVP- GNPs biosensors can detect dilution 1010 of EHV-1 with mean Cycle threshold (Ct) 11.7 and 9.6, respectively and one copy as limit of detection, while citrate- GNPs and PVP- GNPs biosensors can detect dilution 1010 of EHV-4 with mean Ct 10.5and 9.2, respectively and one copy as limit of detection. These findings were confirmed by testing 87 different clinical samples, 4 more samples were positive with multiplex GNPs biosensors rPCR than multiplex rPCR. Multiplex citrate- GNPs and PVP- GNPs biosensors for EHV-1 and EHV-4 are a significant breakthrough in the diagnosis of these virus types. These biosensors offer high sensitivity and specificity, allowing for the accurate detection of the target viruses at very low concentrations and improve the early detection of EHV-1 and EHV-4, leading to faster control of infected animals to prevent the spread of these viruses.

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Section: Discussionmentioning

confidence: 99%

Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses

Ghoniem

ElZorkany²,

Hagag

et al. 2023

Preprint

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show abstract

“…Au NPs Au NPs are the most well-studied nanoparticles, and their interesting chemical and photophysical properties make them an integral part of nanoscience and ideal for biological and other commercial laboratories, which contain non-toxic, good biocompatibility, and unique chemical and optoelectronic properties. For example, Au NPs are characterized by adjustable size and physical size, catalytic activity, high surface volume ratio, high stability, easy synthesis and surface modification, and strong light absorption and scattering properties [5,14]. Figure 1 shows transmission electron microscopy (TEM) images of Au NPs in different morphologies [15,16].…”

Section: Utilizing Different Nanomaterials To Enhance Pcr Effectsmentioning

confidence: 99%

Application of Nanomaterials to Enhance Polymerase Chain Reaction

et al. 2022

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Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology. However, it suffers from low efficiency and specificity problems, false positive results, and so on. Although many conditions can be optimized to increase PCR yield, such as the magnesium ion concentration, the DNA polymerases, the number of cycles, and so on, they are not all-purpose and the optimization can be case dependent. Nano-sized materials offer a possible solution to improve both the quality and productivity of PCR. In the last two decades, nanoparticles (NPs) have attracted significant attention and gradually penetrated the field of life sciences because of their unique chemical and physical properties, such as their large surface area and small size effect, which have greatly promoted developments in life science and technology. Additionally, PCR technology assisted by NPs (NanoPCR) such as gold NPs (Au NPs), quantum dots (QDs), and carbon nanotubes (CNTs), etc., have been developed to significantly improve the specificity, efficiency, and sensitivity of PCR and to accelerate the PCR reaction process. This review discusses the roles of different types of NPs used to enhance PCR and summarizes their possible mechanisms.

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“…Therefore, complementary methods have been developed to improve the detection of Bacillus cereus by PCR. One of these methods is the use of gold nanoparticles (AuNPs) in combination with conventional PCR as enhancers promising results in terms of sensitivity and specificity [14,28].…”

Section: Introductionmentioning

confidence: 99%

A recent technique for the detection of Bacillus cereus in milk

Elgushi,

Mohammed,

Elbarbary

et al. 2023

Egypt. J. Chem.

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The occurrence of Bacillus cereus in milk and dairy products is attributed to deficient hygiene protocols, consequently resulting in two gastrointestinal illnesses: rare emetic sickness and diarrheal illness triggered by enterotoxins. The current study aimed to overcome the limitations of PCR in detecting low bacterial concentrations through the incorporation of unmodified gold nanoparticles (GNPs) in polymerase Chain Reaction (PCR). Bacillus cereus in milk was analyzed using the GNPs-assisted PCR method to detect the nheA gene in comparison to conventional PCR and SYBRGreen qPCR. The nheA gene is a key virulence factor that encodes a protein involved in the generation of non-hemolytic enterotoxins. The result showed adding GNPs to PCR reactions boosted DNA yields and enabled detection down to 10 2 copies of Bacillus cereus DNA, versus 10 3 with standard PCR. GNPs-assisted PCR detected Bacillus cereus for spiked milk samples at 10 2 cfu/ml, while conventional PCR required 10 3 cfu/ml. SYBRGreen qPCR also achieved detection at 10 2 DNA copies and 10 2 cfu/ml for spiked milk. GNPs-assisted PCR specifically amplified Bacillus cereus, not other bacteria like Bacillus subtilis, E. coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella, demonstrating assay specificity. Overall, GNPs improved PCR sensitivity for Bacillus cereus detection.

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The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins

Cited by 12 publications

References 108 publications

Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses

Development of multiplex gold nanoparticles biosensors for ultrasensitive detection and genotyping of equine herpes viruses

Application of Nanomaterials to Enhance Polymerase Chain Reaction

A recent technique for the detection of Bacillus cereus in milk

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