The Interaction Between Niche and Hematopoietic Stem Cells

Wang, Chaoyu; Tian, Chen; Zhang, Yizhuo

doi:10.1007/s12288-016-0639-1

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…The specific microenvironment for the survival of HSCs is termed as 'niche' [11,12]. Scientists have done extensive research on the mechanisms of their maintenance and the complex molecular and cellular composition, but the factors that currently regulate HSCs regulation are still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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Background: The present study investigated the effects of microvascular endothelial cells (MECs) on the chemotaxis, adhesion and proliferation of bone marrow hematopoietic stem cells (HSCs) ex vivo. Methods and Results: MECs were collected from the lung tissue of C57BL/6 mice, and HSCs were isolated with immunomagnetic beads from bone marrow of GFP mice. MECs and HSCs were co-cultured with or without having direct cell–cell contact in Transwell device for the measurement of chemotaxis and adhesion of MECs to HSCs. Experimental results indicate that the penetration rate of HSCs from the Transwell upper chamber to lower chamber in ‘co-culture’ group was significantly higher than that of ‘HSC single culture’ group. Also, the HSCs in co-culture group were all adherent at 24 h, and the co-culture group with direct cell–cell contact had highest proliferation rate. The HSC number was positively correlated with vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels in supernatants of the culture. Conclusions: Our study reports that MECs enhance the chemotaxis, adhesion and proliferation of HSCs, which might be related to cytokines SDF-1 and VEGF secreted by MECs, and thus MECs enhance the HSC proliferation through cell–cell contact. The present study revealed the effect of MECs on HSCs, and provided a basis and direction for effective expansion of HSCs ex vivo.

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Section: Introductionmentioning

confidence: 99%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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“…For hematopoietic cells in the bone marrow microenvironment (3) cells may enter a quiescent state or a proliferative state depending on the specific environmental niche (3). The discovery of the micro environmental effect on the fate of stem cells (7)(8)(9)(10)(11)(12)(13) gave rise to the concept of "quiescent"-now widely considered to be a "dormant-like cell" in the bone marrow (14,15). Studies on the interplay between microenvironment and cancer proliferation have suggested additional prognostic factors such as cell cycle and metabolismrelated chemo-resistant heterogeneity in cancers (16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA Profiling Identifies Diverse Cellular Responses to EWSR1-FLI1 Down-regulation in Ewing Sarcoma

Khoogar

Lawlor

Ignatius

et al. 2019

Preprint

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Single-cell analyses provide insight into time dependent behaviors in response to dynamic changes of oncogene expression. We developed an unbiased approach to study gene expression variation using a model of cellular dormancy induced via EWSR1-FLI1 down-regulation in Ewing sarcoma (EWS) cells. We propose that variation in the expression of EWSR1-FLI1 over time determines cellular responses. Cell state and functions were assigned using random forest feature selection in combination with machine learning. Notably, three distinct expression profiles were uncovered contributing to Ewing sarcoma cell heterogeneity. Our predictive model identified ~1% cells in a dormant-like state and ~2-4% with higher stem-like and neural stem-like features in an exponentially proliferating EWS cell line and EWS xenografts. Following oncogene knockdown, cells re-entering the proliferative cycle have greater stem-like properties, whereas for those remaining quiescent, FAM134B-dependent dormancy provides a survival mechanism. We also show cell cycle heterogeneity related to EWSR1-FLI1 expression as an independent feature driving cancer heterogeneity, and drug resistance.SIGNIFICANCE We show that time-dependent changes induced by suppression of oncogenic EWSR1-FLI1 induces dormancy, with different subpopulation dynamics, including stem-like characteristics and prolonged dormancy. Cells with these characteristics are identified in exponentially growing cell populations and confer drug resistance, and could potentially contribute to metastasis or late recurrence in patients.

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“…In vivo stem cells live in a complex microenvironment called stem cell niche [1][2][3]. Stem cell niche can affect stem cell behavior and regulate stem cell fate by multiple signals.…”

mentioning

confidence: 99%

Extracellular Matrix Plays an Important Role in Stem Cell Biology

Wen¹,

Hu²,

Wu³

2018

J Stem Cell Res Ther

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The Interaction Between Niche and Hematopoietic Stem Cells

Cited by 5 publications

References 38 publications

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Single-cell RNA Profiling Identifies Diverse Cellular Responses to EWSR1-FLI1 Down-regulation in Ewing Sarcoma

Extracellular Matrix Plays an Important Role in Stem Cell Biology

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