The interaction between PAMAM G3.5 dendrimer, Cd2+, dendrimer–Cd2+ complexes and human serum albumin

Sękowski, Szymon; Kaźmierczak, Andrzej; Mazur, Janusz; Przybyszewska, Magdalena; Zaborski, Marian; Shcharbin, Dzmitry; Gabryelak, Teresa

doi:10.1016/j.colsurfb.2008.11.006

Cited by 17 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it was safer to use lower G2.5 concentration when testing its activity. The main assumption of this study was based on the proven chelating properties of half-generations PAMAM dendrimers [3][4][5]. We believed that G2.5 is able to successfully bind excess of Ca 2+ ions and thus limits their input to mitochondria.…”

Section: Discussionmentioning

confidence: 99%

“…Neutral concentration of G2.5 was assessed experimentally in our laboratory, but we did not check its chelating activity towards Ca 2+ . Based only on the literature data, we hoped that even low generations of PAMAMs can effectively bind metal ions, such as: Ag + , Cu 2+ , Zn 2+ , Fe 2+ , and Fe 3+ [5,15]. Nevertheless, so far, the investigation of binding Ca 2+ by PAMAM dendrimers was not proven using appropriate experimental techniques (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, some papers reported that PAMAM dendrimers were able to bind metal ions [3][4][5]. It was evidenced using different methods that ions can be chelated not only buy dendrimers' terminal groups but also by their internal parts, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…was also revealed that anionic dendrimers could form complexes with metals which interact much less strongly with cell biomacromolecules than pure dendrimer or pure metal [5].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Łabieniec-Watała¹,

Siewiera²

2013

CellBio

View full text Add to dashboard Cite

Seasonal variations play an essential role in the metabolism, behavior and activity of the laboratory animals. This study was aimed to examine whether mitochondrial function can be influenced by the seasonal changes and how large is the impact of these fluctuations on experiments with using animal models and further results interpretation. Liver mitochondria were isolated from male Wistar rats and exposed to calcium ions, PAMAM dendrimers G2.5 or their combination: (Ca 2+ ) and dendrimer. The scientific hypothesis assumed that dendrimer G2.5 is able to limit the detrimental effect of Ca 2+ ions on mitochondria function, possibly through affecting the following parameters: calcium transport, mitochondrial potential and membrane fluidity. The activity of mitochondria was monitored using fluorescent labels. The changes in calcium transport were detected using Calcium Green 5-N, the mitochondrial membrane potential and membrane fluidity were elucidated using JC-1 and DPH, respectively. The experiments were carried out during the autumn (October/November) or during the spring (May/June). The obtained data emphasize the effect of seasonal differences on liver mitochondria originating from laboratory animals and outline the importance of planning the experiments during the same seasonal period in order to receive objective and reliable results in the future. Finally, it was revealed the neutral effect of G2.5 dendrimer on mitochondria and its inability to protect mitochondria against overload of calcium ions regardless of seasonality. It was also evidenced that liver mitochondria isolated from autumn-derived animals were more sensitive to calcium and/or dendrimer exposure in comparison with mitochondria isolated from animals investigated during the spring.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…was also revealed that anionic dendrimers could form complexes with metals which interact much less strongly with cell biomacromolecules than pure dendrimer or pure metal [5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Łabieniec-Watała¹,

Siewiera²

2013

CellBio

View full text Add to dashboard Cite

show abstract

“…Poly(amidoamine) (PAMAM) type dendrimers exhibit an excellent capability to bind metal ions and form stable coordination complexes [14,15]. These nanoparticles are globular molecules with uniform structure (Figure 1), low dispersion and good water solubility [16,17].…”

Section: Introductionmentioning

confidence: 99%

Computational study of the complexation of metals ions with poly(amidoamine) PAMAM G0 dendrimers

Camarada

Zúñiga

Alzate‐Morales

et al. 2014

Chemical Physics Letters

View full text Add to dashboard Cite

The Effects of Paclitaxel in the Combination of Diamond Nanoparticles on the Structure of Human Serum Albumin (HSA) and Their Antiproliferative Role on MDA-MB-231cells

2020

View full text Add to dashboard Cite

The interaction between PAMAM G3.5 dendrimer, Cd2+, dendrimer–Cd2+ complexes and human serum albumin

Cited by 17 publications

References 29 publications

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Computational study of the complexation of metals ions with poly(amidoamine) PAMAM G0 dendrimers

The Effects of Paclitaxel in the Combination of Diamond Nanoparticles on the Structure of Human Serum Albumin (HSA) and Their Antiproliferative Role on MDA-MB-231cells

Contact Info

Product

Resources

About