The Interaction between Uranium and Bovine Serum Albumin

Abstract

There is mounting scientific evidence from both animal, and in vitro studies that suggest deleterious effects on human health from inhaled uranium. Serum albumin plays a significant role in carrying, buffering and nutrition. In order to study the interaction between uranium and proteins, as well as the in vivo mechanism of uranium metabolic pathway and biological effect, the orthogonal design experiments in four levels and the three factors were carried out in this research. The results showed that the best reaction conditions between uranyl ions and BSA (Bovine Serum Albumin) was pH3.0, when uranyl ions concentration was 0.60 mg/mL, BSA concentrations was 0.45 mg/mL. In this research the FTIR (Fourier-transform infrared) spectra was also used to analysis the interaction between the uranium and BSA. The FTIR spectra indicated that the -O-H, -C=O of BSA contributed to the major binding groups and the uranyl ions ccould change the BSA conformation of BSA when interaction each other under different pH conditions.

The feature of migration and deoxygenation of radionuclides U(Ⅵ) on genetically recombined bacteria W1

“…Both 623.52cm −1 and 538.64cm −1 are the out-of-plane bending vibration peaks of PO4 3-, but the absorption band of 538.64 cm −1 is completely covered by the absorption band of 623.52 cm −1 . there is a characteristic vibrating absorption peak of antisymmetric carboxylic acid O-H stretch corresponding to UO2 2+ at the 918.12 cm −1 (Liu et al, 2011). The vicinity of 1339.15 cm -1 and 1070. cm -1 are attributed to the asymmetric methyl and the C-N bond of aliphatic amine vibrating absorption, respectively.…”

Research Progress on the Impact of Nuclear Radiation in Food on Human Health

“…Similarly, U(VI) interacts with serum proteins. Fourier-transform infrared spectroscopy (FTIR) shows that uranium ions can change the conformation of bovine serum albumin (BSA) under different pH conditions, indicating interaction between them [18].…”