The interaction of bovine prothrombin fragment 1 with metal ions. An immunological approach to kinetic and equilibrium studies.

Calcium-dependent .alpha.-helical structure in osteocalcin

“…Ca2+ mediates a structural transition in prothrombin by a relatively high-affinity interaction (Kd 2 X 10"4 M) of two Ca2+ ions with two specific pairs of Gla residues and weaker binding of about six Ca2+ ions at other Gla sites (Nelsestuen, 1976;Bloom & Mann, 1978;Marsh et al, 1981;Furie et al, 1979). This binding promotes adoption of the -helical conformation and facilitates adsorption of prothrombin to acidic phospholipid surfaces (Nelsestuen, 1976;Nelsestuen et al, 1979;Tuhy et al, 1979;Madar et al, 1980).…”

Immunochemical studies of conformational alterations in bone .gamma.-carboxyglutamic acid containing protein

“…Ca2+ mediates a structural transition in some of the vitamin K dependent blood-clotting proteins, including prothrombin (Nelsestuen, 1976; Stenflo, 1977; Bloom & Mann, 1978;Tuhy et al, 1979;Madar et al, 1980) and factor X (Keyt et al, 1982). Recently, a Ca2+-dependent confor-1 Abbreviations: BGP, bone Gla-containing protein (also called osteocalcin); Gla, 7-carboxyglutamic acid; EDTA, ethylenediaminetetraacetic acid; Tris, tris(hydroxymethyl)aminomethane; BSA, bovine serum albumin.…”

Conformation-specific monoclonal antibodies directed against the calcium-stabilized structure of human prothrombin

“…to the study of conformational motility of polypeptides (Sachs et al, 1972;Furie et al, 1975;Hurrell et al, 1977), pathways of protein folding (Chavez & Scheraga, 1977; Creighton et al, 1978), distinction of abnormal and mutant proteins (Young et al, 1975;Blanchard et al, 1979), and the effects of ligand binding on protein conformation (Furie et al, 1975; ; Dean & Schechter, 1979;Tai et al, 1980;Madar et al" 1980).…”