The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy

Szekeres, Gergő Péter; Krekic, Szilvia; Miller, Rebecca L.; Mero, Mark; Pagel, Kevin; Heiner, Zsuzsanna

doi:10.1039/d1cp01975a

Cited by 5 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, recent research suggests that CS is able to interact with the lipid monolayer. Notably, in the presence of Ca 2+ , the head groups together with the head-group-bound water molecules in the dipalmitoyl phosphatidylcholine monolayer, which are significantly impacted by the interaction with CS, indicating that CS displays a linearly chiral secondary structure at a charged biological interface in solution and is likely to form a helical coil [147]. Aside from CS together with other substrates acting as the conjugate status, it can be considered as a mixture with its original status, akin to the mixtures of CS and collagen, CS and glucosamine, and CS and hyaluronic acid, which will be summarized in Section 4.2.…”

Section: The Complex Properties Of Csmentioning

confidence: 99%

A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Shen,

Guo,

Wang

et al. 2023

Molecules

View full text Add to dashboard Cite

Chondroitin sulfate (CS) is a natural macromolecule polysaccharide that is extensively distributed in a wide variety of organisms. CS is of great interest to researchers due to its many in vitro and in vivo functions. CS production derives from a diverse number of sources, including but not limited to extraction from various animals or fish, bio-synthesis, and fermentation, and its purity and homogeneity can vary greatly. The structural diversity of CS with respect to sulfation and saccharide content endows this molecule with distinct complexity, allowing for functional modification. These multiple functions contribute to the application of CS in medicines, biomaterials, and functional foods. In this article, we discuss the preparation of CS from different sources, the structure of various forms of CS, and its binding to other relevant molecules. Moreover, for the creation of this article, the functions and applications of CS were reviewed, with an emphasis on drug discovery, hydrogel formation, delivery systems, and food supplements. We conclude that analyzing some perspectives on structural modifications and preparation methods could potentially influence future applications of CS in medical and biomaterial research.

show abstract

Section: The Complex Properties Of Csmentioning

confidence: 99%

A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Shen,

Guo,

Wang

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…In a proof-of-concept experiment series, we recently demonstrated that it is possible to study lipid-GAG interactions at the air-liquid interface within a DPPC monolayer on aqueous subphase containing CS and Ca 2+ ions (Fig. 2c ) at physiologically relevant salt- and sub-micromolar analyte concentrations [ 39 ]. During this interaction, the structural changes of the DPPC monolayer were monitored by VSFG spectroscopy.…”

Section: Emerging Bioanalytical Tools For the Characterization Of Gag-lipid Interactionsmentioning

confidence: 99%

“…2c ). These results led to the conclusion that long CS chains form an organized layer below the DPPC monolayer, at which the CS aligns into a chiral secondary structure, most probably a helical coil [ 39 ]. The folding of GAG chains into helical segments was shown previously in solution, but has so far not been demonstrated at interfaces.…”

Section: Emerging Bioanalytical Tools For the Characterization Of Gag-lipid Interactionsmentioning

confidence: 99%

Analytical challenges of glycosaminoglycans at biological interfaces

2021

Self Cite

View full text Add to dashboard Cite

The analysis of glycosaminoglycans (GAGs) is a challenging task due to their high structural heterogeneity, which results in diverse GAG chains with similar chemical properties. Simultaneously, it is of high importance to understand their role and behavior in biological systems. It has been known for decades now that GAGs can interact with lipid molecules and thus contribute to the onset of atherosclerosis, but their interactions at and with biological interfaces, such as the cell membrane, are yet to be revealed. Here, analytical approaches that could yield important knowledge on the GAG-cell membrane interactions as well as the synthetic and analytical advances that make their study possible are discussed. Due to recent developments in laser technology, we particularly focus on nonlinear spectroscopic methods, especially vibrational sum-frequency generation spectroscopy, which has the potential to unravel the structural complexity of heterogeneous biological interfaces in contact with GAGs, in situ and in real time. Graphical abstract

show abstract

Ion Mobility Mass Spectrometry‐Based Disaccharide Analysis of Glycosaminoglycans

Polewski,

Moon,

Zappe

et al. 2024

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

Glycosaminoglycans (GAGs) are linear and acidic polysaccharides. They are ubiquitous molecules, which are involved in a wide range of biological processes. Despite being structurally simple at first glance, with a repeating backbone of alternating hexuronic acid and hexosamine dimers, GAGs display a highly complex structure, which predominantly results from their heterogeneous sulfation patterns. The commonly applied method for compositional analysis of all GAGs is "disaccharide analysis." In this process, GAGs are enzymatically depolymerized into disaccharides, derivatized with a fluorescent label, and then analysed through liquid chromatography. The limiting factor in the high throughput analysis of GAG disaccharides is the time‐consuming liquid chromatography. To address this limitation, we here utilized trapped ion mobility‐mass spectrometry (TIM‐MS) for the separation of isomeric GAG disaccharides, which reduces the measurement time from hours to a few minutes. A full set of disaccharides comprises twelve structures, with eight possessing isomers. Most disaccharides cannot be differentiated by TIM‐MS in underivatized form. Therefore, we developed chemical modifications to reduce sample complexity and enhance differentiability. Quantification is performed using stable isotope labelled standards, which are easily available due to the nature of the performed modifications.

show abstract

The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy

Abstract: The first vibrational sum-frequency generation (VSFG) spectra of chondroitin sulfate (CS) interacting with dipalmitoyl phosphatidylcholine (DPPC) at air-liquid interface are reported here, collected at a laser repetition rate of 100...

Cited by 5 publications

References 34 publications

A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Analytical challenges of glycosaminoglycans at biological interfaces

Ion Mobility Mass Spectrometry‐Based Disaccharide Analysis of Glycosaminoglycans

Contact Info

Product

Resources

About