The interaction of CsWRKY4 and CsOCP3 with CsICE1 regulates CsCBF1/3 and mediates stress response in tea plant (Camellia sinensis)

Peng, Jing; Li, Nana; Di, Taimei; Ding, Changqing; Li, Xiaoman; Wu, Yedie; Hao, Xinyuan; Yang, Yajun; Wang, Xinchao; Wang, Lu

doi:10.1016/j.envexpbot.2022.104892

Cited by 13 publications

(6 citation statements)

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“…We confirmed that the cold stress treatment was successful by examining the induction of cold stress marker genes. Consistent with previous studies [ 6 , 33 ], we found that CsCBF1 , CsCBF2 , CsCBF3 , and CsCBF4 had low expression levels under normal temperature conditions and were induced by cold stress treatment ( Figure 3 A). In the non-cold stress treatment, 90 genes, including 43 up-regulated and 47 down-regulated genes, were differentially expressed genes (DEGs) in the AsA treatment (NV) compared with the normal temperature control (NO) (NV vs. NO) ( Figure 3 B, Table S3 ).…”

Section: Resultssupporting

confidence: 93%

“…Cold stress is one of the harshest abiotic stresses that negatively affects both the yield and quality of tea [ 35 ]. Therefore, it is crucial to develop approaches for improving the cold tolerance of tea plants [ 6 , 33 , 36 , 37 , 38 , 39 , 40 ]. Natural metabolites have recently been widely used to improve plant growth, crop yield, and stress resistance [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling

Cao

Wang

et al. 2023

IJMS

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Cold stress is a major environmental factor that adversely affects the growth and productivity of tea plants. Upon cold stress, tea plants accumulate multiple metabolites, including ascorbic acid. However, the role of ascorbic acid in the cold stress response of tea plants is not well understood. Here, we report that exogenous ascorbic acid treatment improves the cold tolerance of tea plants. We show that ascorbic acid treatment reduces lipid peroxidation and increases the Fv/Fm of tea plants under cold stress. Transcriptome analysis indicates that ascorbic acid treatment down-regulates the expression of ascorbic acid biosynthesis genes and ROS-scavenging-related genes, while modulating the expression of cell wall remodeling-related genes. Our findings suggest that ascorbic acid treatment negatively regulates the ROS-scavenging system to maintain ROS homeostasis in the cold stress response of tea plants and that ascorbic acid’s protective role in minimizing the harmful effects of cold stress on tea plants may occur through cell wall remodeling. Ascorbic acid can be used as a potential agent to increase the cold tolerance of tea plants with no pesticide residual concerns in tea.

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Section: Resultssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling

Cao

Wang

et al. 2023

IJMS

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“…After 48 h, the LCI assay was performed using a chemiluminescence image analysis system of Tanon (Shanghai, China). These N. benthamiana specimens were then collected for the Dual-Luc assay using the Dual-Luciferase Reporter System of Promega (Madison, WI, USA) according to the manufacturer’s instructions, as previously described [ 46 ]. Six independent biological replicates were taken.…”

Section: Methodsmentioning

confidence: 99%

CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants

Peng

et al. 2023

IJMS

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Photosystem II repair in chloroplasts is a critical process involved in maintaining a plant’s photosynthetic activity under cold stress. FtsH (filamentation temperature-sensitive H) is an essential metalloprotease that is required for chloroplast photosystem II repair. However, the role of FtsH in tea plants and its regulatory mechanism under cold stress remains elusive. In this study, we cloned a FtsH homolog gene in tea plants, named CsFtsH5, and found that CsFtsH5 was located in the chloroplast and cytomembrane. RT-qPCR showed that the expression of CsFtsH5 was increased with leaf maturity and was significantly induced by light and cold stress. Transient knockdown CsFtsH5 expression in tea leaves using antisense oligonucleotides resulted in hypersensitivity to cold stress, along with higher relative electrolyte leakage and lower Fv/Fm values. To investigate the molecular mechanism underlying CsFtsH5 involvement in the cold stress, we focused on the calcineurin B-like-interacting protein kinase 11 (CsCIPK11), which had a tissue expression pattern similar to that of CsFtsH5 and was also upregulated by light and cold stress. Yeast two-hybrid and dual luciferase (Luc) complementation assays revealed that CsFtsH5 interacted with CsCIPK11. Furthermore, the Dual-Luc assay showed that CsCIPK11-CsFtsH5 interaction might enhance CsFtsH5 stability. Altogether, our study demonstrates that CsFtsH5 is associated with CsCIPK11 and plays a positive role in maintaining the photosynthetic activity of tea plants in response to low temperatures.

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“…Mechanistically, jasmonate-zim-domain protein 1/4 (JAZ1/4) can also inhibit the CBF translational activity by interacting with ICE1/2 in Arabidopsis (Hu et al, 2013a). The most recent study in tea plants found that CsWRKYs (CsWRKY29 and CsWRKY37) conferred plants cold tolerance, and CsWRKY4/ CsOCP3 (OVEREXPRESSOR OF CATIONIC PEROXIDASE 3) interacted with CsICE1 and inhibited its transcriptional activation on CsCBF1/3, demonstrating the relevance of CsCBF cascade pathway on cold tolerance of tea plants (Peng et al, 2022;Zhao et al, 2022).…”

Section: Transcriptional and Post-transcriptional Regulation Of Ice-c...mentioning

confidence: 99%

Recent progress and perspectives on physiological and molecular mechanisms underlying cold tolerance of tea plants

et al. 2023

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Tea is one of the most consumed and widely planted beverage plant worldwide, which contains many important economic, healthy, and cultural values. Low temperature inflicts serious damage to tea yields and quality. To cope with cold stress, tea plants have evolved a cascade of physiological and molecular mechanisms to rescue the metabolic disorders in plant cells caused by the cold stress; this includes physiological, biochemical changes and molecular regulation of genes and associated pathways. Understanding the physiological and molecular mechanisms underlying how tea plants perceive and respond to cold stress is of great significance to breed new varieties with improved quality and stress resistance. In this review, we summarized the putative cold signal sensors and molecular regulation of the CBF cascade pathway in cold acclimation. We also broadly reviewed the functions and potential regulation networks of 128 cold-responsive gene families of tea plants reported in the literature, including those particularly regulated by light, phytohormone, and glycometabolism. We discussed exogenous treatments, including ABA, MeJA, melatonin, GABA, spermidine and airborne nerolidol that have been reported as effective ways to improve cold resistance in tea plants. We also present perspectives and possible challenges for functional genomic studies on cold tolerance of tea plants in the future.

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The interaction of CsWRKY4 and CsOCP3 with CsICE1 regulates CsCBF1/3 and mediates stress response in tea plant (Camellia sinensis)

Cited by 13 publications

References 50 publications

Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling

Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling

CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants

Recent progress and perspectives on physiological and molecular mechanisms underlying cold tolerance of tea plants

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