The interaction of mitochondrial ATPase with an alkylating ATP‐analog

The proton-ATPase of bacteria and mitochondria

“…As noted before, the essential tyrosyl and glutamyl residues are conserved in homologous regions of sequence in beef heart Flfi subunit and E. coli Flfl subunit, and they are also conserved in chloroplast Flfl subunit (Zurawski et al, 1982). Two other ATP analogs with reactive substituents in position approximating that of the fl-7 phosphoryl groups of ATP have been shown to inhibit soluble F~ activity and to covalently label the fl subunit (Budker et al, 1977;Drutsa et al, 1979). The inhibitory, reactive analog of Pi(4-azido,2-nitrophenylphosphate) has also been shown to label the fl subunit (Lauquin et al, 1980).…”

Chemical Probes of the Mitochondrial Atp Synthesis and Translocation

“…Careful chemical determinations showed that dial ATP has a tendency to lose its triphosphate group and to generate an adenine containing compound with a highly reactive conjugated aldehyde group, ex plaining the formation of the stable conjugated Schiff base with FI (218, 219). An etheno derivative of dial ATP (dial e-ATP) has been reported to inactivate beef liver F" with full inactivation being attained for 1 mol reagent bound per mol F, at the level of the 13 subunit Two other affinity labels have been used with mitochondrial F" namely the mixed anhydride formed by condensation of ATP and mesitylene carboxylic acid (222) and the A TP -'Y-4(N -2-chloroethy I-N -methy lamino) benzoy lamidate (223). In the presence of Mg 2 + , dial ATP was hydrolyzed to give dial-ADP, indicating that dial ATP binds at the active site of F ,.…”

Affinity labelling of yeast hexokinase with benzylamide derivatives of adenosine mono‐ and triphosphates bearing an alkylating group

“…This is the first example of the active site of such type for alkylating analogs of NTPs. C1RCH2NHpppA inactivates creatine kinase [ 14] and mitochondrial ATP-ase [ 15 ] and a similar dTTP derivative readily reacts with DNA polymerase I [16] and RNA-dependent DNA polymerase [17].…”