The interaction of saccharides with lipid bilayer vesicles: stabilization during freeze-thawing and freeze-drying

Strauss, George; Schurtenberger, Peter; Häuser, H.

doi:10.1016/0005-2736(86)90303-2

Cited by 165 publications

(64 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The obvious interpretation of this is that the sugar molecules increase the area through intercalation between the lipid head groups. Other experimental approaches have reached analogous conclusions in work on lipid bilayers (19,20,24,32), and Viera et al (33) found that trehalose that was bound to a bilayer during dehydration remained associated for hours upon rehydration in buffer without sugar. Most recently, the interaction hypothesis has been supported by many molecular dynamics simulations (see, e.g., refs.…”

mentioning

confidence: 73%

“…Investigations of fully hydrated membranes show an interesting tendency to fall into two groups with mutually conflicting conclusions. Thus, many investigations have suggested direct (favorable) interaction of sugars and the phospholipid interface (16)(17)(18)(19)(20)(21)(22)(23), and it is obvious that such interactions could be the origin of sugar effects, for example, through interlocking of several lipids molecules that simultaneously hydrogen bond to the same disaccharide molecule (24). Other stabilizing consequences of sugar binding have been put forward, and in this paper, we collectively refer to this interpretation as the "interaction hypothesis."…”

mentioning

confidence: 99%

See 1 more Smart Citation

Reconciliation of opposing views on membrane–sugar interactions

Andersen

Wang

Arleth

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

141

177

View full text Add to dashboard Cite

It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used smallangle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above ∼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar-membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.membrane interface | membrane structure | preferential binding | preferential exclusion | interaction free energy S mall sugars such as the disaccharides sucrose and trehalose are among the so-called osmolytes (1) or compensatory solutes (2), which are accumulated in response to environmental stress in virtually all taxa. Their function is to act as inert regulators of the osmotic pressure, but they also optimize the physical properties of the cytosol (3) and stabilize biomolecular conformations against cold, drought, and heat (4-7). The same small carbohydrates have also proven useful in vitro as protectants or excipients for biopreservation (8). Many reports have shown that membranous structures are particularly stabilized by small sugars (4, 6, 9), but the definition of stabilization covers a wide range of biological and physical parameters. Thus, studies on intact cells have documented improved survival following exposure to heat, cold, drought, or chemical stressors (6,10,11). Other works have analyzed stabilization on the basis of phenomenological properties of model membranes, for example, the leakage or intermixing of probes in liposomes (12, 13). Finally, stability has been discussed with respect to rigorous physical parameters such as the structure or mechanical properties of lipid bilayers (14, 15). The current work addresses membrane dimensions and the thermodynamics of interaction with the purpose of elucidating fundamental aspects of membrane-sugar interrelationships. The different observations of sugar stabilization have sparked a large number of studies on sugars and model membranes (usually phospholipid bilayers) over the past 30 y. Investigations of fully hydrated membranes show an interesting tendency to fall into two groups with mutually conflicting conclusions. Thus, many investigations have suggested direct (favorable) interaction of sugars and the phospholipid interface (16-23), and it is obvious that such interactions could be the origin of sugar effects, for example, through int...

show abstract

mentioning

confidence: 73%

mentioning

confidence: 99%

Reconciliation of opposing views on membrane–sugar interactions

Andersen

Wang

Arleth

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

141

177

View full text Add to dashboard Cite

show abstract

“…Cryoprotectants provide an energy source for spermatozoa and maintain the osmotic pressure by forming hydrogen bonds with membrane phospholipids and sugars. This reduces membrane damage and minimizes membrane destabilization during freezing and thawing [39]. This method can preserve spermatozoa for a long time and, after thawing, sperm motility and fertilization ability is restored.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic reprogramming of embryos derived from sperm frozen at −20°C

Chao

Jin

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at 20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at 20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI. spermatozoa freezing, ICSI, epigenetic, histone modification, DNA methylation Citation:Chao S B, Li J C, Jin X J, et al. Epigenetic reprogramming of embryos derived from sperm frozen at 20°C.

show abstract

“…36,37 In addition, drug particles coated by the phospholipids were interdispersed and further stabilized in the aminoclay matrix to prevent aggregation during lyophilization. 38,39 Aminoclay could also enhance the wettability. 12,13,23 Considering the continuous drug movement across the intestinal membrane during the absorption process in vivo, the supersaturation achieved by ALC formulations could be maintained for longer time in vivo.…”

Section: Optimization Of Formulation Variablesmentioning

confidence: 99%

“…Furthermore, drug particles coated by the phospholipids were dispersed in the aminoclay matrix, leading to the prevention of particle aggregation during lyophilization. 38,39 Therefore, the enhanced drug dissolution from ALC formulation may be explained by the reduced particle size and increased surface area. The improved surface-wetting by the phospholipids and aminoclay in the formulation may also contribute to further enhancement in drug dissolution.…”

Section: Dissolution Of the Optimized Alc Formulationmentioning

confidence: 99%

Aminoclay–lipid hybrid composite as a novel drug carrier of fenofibrate for the enhancement of drug release and oral absorption

Yang

Shao

Han

2016

IJN

View full text Add to dashboard Cite

This study aimed to prepare the aminoclay–lipid hybrid composite to enhance the drug release and improve the oral bioavailability of poorly water-soluble fenofibrate. Antisolvent precipitation coupled with an immediate freeze-drying method was adopted to incorporate fenofibrate into aminoclay–lipid hybrid composite (ALC). The optimal composition of the ALC formulation was determined as the ratios of aminoclay to krill oil of 3:1 (w/w), krill oil to fenofibrate of 2:1 (w/w), and antisolvent to solvent of 6:4 (v/v). The morphological characteristics of ALC formulation were determined using scanning electron microscopy, differential scanning calorimetry, and X-ray powder diffraction, which indicated microcrystalline state of fenofibrate in ALC formulation. The ALC formulation achieved almost complete dissolution within 30 minutes, whereas the untreated powder and physical mixture exhibited less than 15% drug release. Furthermore, ALC formulation effectively increased the peak plasma concentration ( C max ) and area under the curve (AUC) of fenofibric acid (an active metabolite) in rats by approximately 13- and seven-fold, respectively. Furthermore, ALC formulation exhibited much lower moisture sorption behavior than the lyophilized formulation using sucrose as a cryoprotectant. Taken together, the present findings suggest that ALC formulation is promising for improving the oral absorption of poorly soluble fenofibrate.

show abstract

The interaction of saccharides with lipid bilayer vesicles: stabilization during freeze-thawing and freeze-drying

Cited by 165 publications

References 19 publications

Reconciliation of opposing views on membrane–sugar interactions

Reconciliation of opposing views on membrane–sugar interactions

Epigenetic reprogramming of embryos derived from sperm frozen at −20°C

Aminoclay–lipid hybrid composite as a novel drug carrier of fenofibrate for the enhancement of drug release and oral absorption

Contact Info

Product

Resources

About

The interaction of saccharides with lipid bilayer vesicles: stabilization during freeze-thawing and freeze-drying

Cited by 165 publications

References 19 publications

Reconciliation of opposing views on membrane–sugar interactions

Reconciliation of opposing views on membrane–sugar interactions

Epigenetic reprogramming of embryos derived from sperm frozen at −20°C

Aminoclay&ndash;lipid hybrid composite as a novel drug carrier of fenofibrate for the enhancement of drug release and oral absorption

Contact Info

Product

Resources

About

Aminoclay–lipid hybrid composite as a novel drug carrier of fenofibrate for the enhancement of drug release and oral absorption