The interaction of TAK1 and TAB1 enhances LPS-induced cytokine release via modulating NF-κB activation (Larimichthys crocea)

Bao, Shiyuan; Sun, Qing-Xue; Yao, Cui-Luan

doi:10.1016/j.fsi.2018.01.005

Cited by 20 publications

(11 citation statements)

References 33 publications

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“…The results showed that the transcription of trTAK1 and trT was constitutive in all tested healthy pompano tissues (Figure 8). Furthermore, the mR of trTAK1 was highly expressed in the liver, intestine and brain, but weakly express the heart and muscle, and the expression of trTAB1 was in agreement with that of g carp TAB1 and large yellow croaker TAB1 [22,25], whereas the highest expressio trTAB2 was observed in the intestine, followed by the liver, brain, gill, spleen, head ney, heart and muscle.…”

Section: Basal Tissue Expression Of Trtak1 Trtab1 and Trtab2supporting

confidence: 60%

“…In mammals, TABs activate TAK1 by forming a kinase complex (TAK-TABs), which is required for the activation of NF-κB [40]. However, the molecular characteristics of TAK1 and TABs in teleost fish, as well as their immune function, remain largely unknown-although they have been reported in grass carp [25], black carp [28] and large yellow croaker [22]. To understand the characteristics and roles of trTAK1, trTAB1 and trTAB2, their gene and amino acid sequences and expression patterns before and after LPS, poly I:C and V. alginolyticus infection were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, TAK1 and/or TAB1 and/or TAB2 have been identified in several marine species, such as large yellow croaker (Larimichthys crocea) [22,23], grass carp (Ctenopharyngodon idellus) [24,25], orange-spotted grouper (Epinephelus coioides) [26,27], black carp (Mylopharyngodon piceus) [28,29], Japanese flounder (Paralichthys olivaceus) [30] and rainbow trout (Oncorhynchus mykiss) [31], providing valuable information about the roles of TAK1, TAB1 and TAB2 in fish innate immunity.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Expression Analysis of TAK1, TAB1 and TAB2 of Golden Pompano (Trachinotus ovatus)

Xie

Lei

et al. 2022

Fishes

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Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), TAK1-binding protein 1 (TAB1) and TAB2 are components of the mitogen-activated protein kinase (MAPK) pathway. In this study, TAK1, TAB1 and TAB2 were characterized from golden pompano (Trachinotus ovatus), a marine fish of great economic value, and named as trTAK1, trTAB1 and trTAB2, respectively. The lengths of the cDNA sequences of the three genes were 2429 bp, 2068 bp and 4229 bp and encoded 575, 506 and 759 amino acids, respectively. The trTAK1, trTAB1 and trTAB2 genes shared high sequence identities and were well clustered with their counterparts from other fish species. Real-time qPCR analysis showed that the three genes were constitutively expressed in all the selected tissues of healthy pompano, and the expression levels of the three genes were significantly up-regulated in head kidney and spleen following Vibrio alginolyticus, lipolysaccharide (LPS) and polyinosinic polycytidylic acid (poly I:C) challenge, indicating their roles in the immune response against pathogens in golden pompano. Our results provide a basis for further study of the functions of these genes in golden pompano.

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Section: Basal Tissue Expression Of Trtak1 Trtab1 and Trtab2supporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Expression Analysis of TAK1, TAB1 and TAB2 of Golden Pompano (Trachinotus ovatus)

Xie

Lei

et al. 2022

Fishes

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“…Previous study showed that LPS‐induced inflammatory responses are associated with the activation of the NF‐κB pathways (Bao, Sun, & Yao, 2018; Iliev, Tom, Martin, & Aleksei, 2013; Li, Sun, et al, 2018). NF‐κB was an effective inflammatory transcript, which can effectively regulate the transcription of inflammatory cytokines (Li, Zhu, et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Amelioration of LPS‐induced inflammatory response and oxidative stress by astaxanthin in Channa argus lymphocyte via activating glucocorticoid receptor signalling pathways

Gao

et al. 2020

Aquac Res

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The present study was conducted to evaluate the effects of astaxanthin (AST) against lipopolysaccharide (LPS)‐induced lymphocyte viability, ultrastructural lesions, apoptosis, oxidative stress and inflammatory responses in Channa argus. Lymphocytes exposed to more than 10 μg/ml LPS alone for 24 hr showed significantly decreased cell viability, elevated nitric oxide (NO) and malondialdehyde (MDA), lactate dehydrogenase (LDH) contents, and increased nuclear factor κB p65 (NF‐κB p65), myeloid differential protein‐88 (MyD88), tumour necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), interleukin‐8 (IL‐8), caspase‐3, caspase‐8 and caspase‐9 gene expression. LPS at a concentration of 10 μg/ml could induce oxidative stress and inflammatory responses in lymphocytes. The activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) were significantly decreased after exposure to 10 μg/ml LPS. Besides, AST strikingly antagonized the LPS‐induced negative effects. AST significantly increased the expression of HSP70, HSP90, IκB‐α, and glucocorticoid receptor (GR) and decreased inflammatory responses. Further study showed that AST can activate GR signalling pathway and inhibit p65 phosphorylation. In addition, AST attenuated LPS‐induced apoptosis, mitochondrial swelling, degeneration and vacuolization. Collectively, these findings suggest that AST has protective roles in LPS‐induced cell damage via modulating GR activation in C. argus lymphocytes.

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“…Real-time PCR was performed in an Applied Biosystems QuantStudio 3 (Thermo Fisher Scientific). β-actin and 5.8S rRNA were employed as endogenous controls for mRNA/lncRNA and miRNA, respectively (51). Primer sequences are displayed in Supplementary table 1.…”

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

The long noncoding RNA MIR122HG is a precursor for miR-122-5p and negatively regulates the TAK1-induced innate immune response in teleost fish

Zheng

Chang

Luo

et al. 2022

Journal of Biological Chemistry

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The interaction of TAK1 and TAB1 enhances LPS-induced cytokine release via modulating NF-κB activation (Larimichthys crocea)

Cited by 20 publications

References 33 publications

Molecular Characterization and Expression Analysis of TAK1, TAB1 and TAB2 of Golden Pompano (Trachinotus ovatus)

Molecular Characterization and Expression Analysis of TAK1, TAB1 and TAB2 of Golden Pompano (Trachinotus ovatus)

Amelioration of LPS‐induced inflammatory response and oxidative stress by astaxanthin in Channa argus lymphocyte via activating glucocorticoid receptor signalling pathways

The long noncoding RNA MIR122HG is a precursor for miR-122-5p and negatively regulates the TAK1-induced innate immune response in teleost fish

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