The interaction of transcription factors controls the spatial layout of plant aerial stem cell niches

Gruel, Jérémy; Deichmann, Julia; Landrein, Benoît; Hitchcock, Thomas; Jönsson, Henrik

doi:10.1038/s41540-018-0072-1

Cited by 29 publications

(29 citation statements)

References 29 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This hypothesis is supported by experimental results and shown plausible by a computational model (Zhou et al, 2018). The hypothesis also aligns with a number of results from earlier, independent studies (Brand et al, 2000;Schoof et al, 2000;Brand et al, 2002;Graf et al, 2010;Schulze et al, 2010;Biedermann and Laux, 2018;Zhou et al, 2018), including a computational model that features an essential role for HAM in control of CLV3 patterns (Gruel et al, 2018). Additionally, the concentration gradient of HAM has been shown to be essential in determining the CLV3 domain in both well-established SAMs and in initiating axillary stem cell niches (Biedermann and Laux, 2018;Zhou et al, 2018), suggesting the important roles of HAM family genes controlling both initiation and maintenance of patterns of gene expression in plant stem cell niches.…”

Section: Introductionsupporting

confidence: 87%

The Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems

et al. 2020

View full text Add to dashboard Cite

In Arabidopsis shoot apical meristems (SAMs), a well-characterized regulatory loop between WUSCHEL (WUS) and CLAVATA3 (CLV3) maintains stem cell homeostasis by regulating the balance between cell proliferation and cell differentiation. WUS proteins, translated in deep cell layers, move into the overlaying stem cells to activate CLV3. The secreted peptide CLV3 then regulates WUS levels through a ligand-receptor mediated signaling cascade. CLV3 is specifically expressed in the stem cells and repressed in the deep cell layers despite presence of the WUS activator, forming an apical-basal polarity along the axis of the SAM. Previously, we proposed and validated a hypothesis that the HAIRY MERISTEM (HAM) family genes regulate this polarity, keeping the expression of CLV3 off in interior cells of the SAM. However, the specific role of each individual member of the HAM family in this process remains to be elucidated. Combining live imaging and molecular genetics, we have dissected the conserved and distinct functions of different HAM family members in control of CLV3 patterning in the SAMs and in the de novo shoot stem cell niches as well.

show abstract

Section: Introductionsupporting

confidence: 87%

The Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Neighboring cells were identified by searching for the overlaps among spheres. Similar multiple spheres based 3D templates have been developed and used in previous modeling research on the transcriptional circuits and gene expression patterns involving WUS, CLV3, HAM, and/or hormone cytokinin in the SAM 8,[46][47][48] .…”

Section: Methodsmentioning

confidence: 99%

“…In the model plant Arabidopsis, several key pathways in control of stem cell homeostasis in the SAMs have been identified [2][3][4] . Among them, the HAIRY MERISTEM (HAM) family GRAS (GAI, RGA and SCR) domain transcription factors play essential roles in determining the specification and proliferation of stem cells in the SAMs [4][5][6][7][8][9] . Two HAM family members, HAM1 and HAM2 proteins, interact with the homeodomain transcription factor WUSCHEL (WUS) and function together with WUS to regulate downstream gene expression and maintain the stem cell homeostasis 7,8 .…”

mentioning

confidence: 99%

“…Among them, the HAIRY MERISTEM (HAM) family GRAS (GAI, RGA and SCR) domain transcription factors play essential roles in determining the specification and proliferation of stem cells in the SAMs [4][5][6][7][8][9] . Two HAM family members, HAM1 and HAM2 proteins, interact with the homeodomain transcription factor WUSCHEL (WUS) and function together with WUS to regulate downstream gene expression and maintain the stem cell homeostasis 7,8 . In addition, HAM1 and HAM2 keep the CLV3 expression off at the inner cell layers to establish an apical-basal polarity of the SAMs and the de novo axillary stem cell niches 9,10 .…”

mentioning

confidence: 99%

See 1 more Smart Citation

A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems

Han

et al. 2020

Nat Commun

View full text Add to dashboard Cite

In multicellular organisms, a long-standing question is how spatial patterns of distinct cell types are initiated and maintained during continuous cell division and proliferation. Along the vertical axis of plant shoot apical meristems (SAMs), stem cells are located at the top while cells specifying the stem cells are located more basally, forming a robust apical-basal pattern. We previously found that in Arabidopsis SAMs, the HAIRY MERISTEM (HAM) family transcription factors form a concentration gradient from the epidermis to the interior cell layers, and this gradient is essential for the stem cell specification and the apical-basal patterning of the SAMs. Here, we uncover that epidermis specific transcription factors, ARABIDOPSIS THALIANA MERISTEM LAYER 1 (ATML1) and its close homolog, define the concentration gradient of HAM in the SAM through activating a group of microRNAs. This study provides a molecular framework linking the epidermis-derived signal to the stem cell homeostasis in plants.

show abstract

“…A biological stress, such as senescence or an acute stress such as abiotic/biotic factors can trigger stem cell formation through altered chromatin conformation and promiscuous expression of transcription factors [32][33][34]. Transcription factors have been shown to function in an important role on the regulation of plant differentiation [35] and development [36]. In monocots, overexpression of various plant transcription factors such as LEAFY COTYLEDON1 [37], WUSCHEL [38], and BABY BOOM [39] have been shown to improve embryo formation and enhanced regeneration [12].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

Parris

et al. 2020

Preprint

View full text Add to dashboard Cite

• Background • Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. • Results • In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5,333 differentially expressed genes (DEG) with 2,534 upregulated and 2,799 downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. • Conclusions • This comparative analysis of NEC and EC transcriptomes gives new insights into the genetic underpinnings of somatic embryogenesis in cotton.

show abstract

The interaction of transcription factors controls the spatial layout of plant aerial stem cell niches

Cited by 29 publications

References 29 publications

The Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems

The Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems

A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

Contact Info

Product

Resources

About