The interaction of wood nanocellulose dressings and the wound pathogen P. aeruginosa

Abstract

Chronic wounds pose an increasingly significant worldwide economic burden (over £1 billion per annum in the UK alone). With the escalation in global obesity and diabetes, chronic wounds will increasingly be a significant cause of morbidity and mortality. Cellulose nanofibrils (CNF) are highly versatile and can be tailored with specific physical properties to produce an assortment of three-dimensional structures (hydrogels, aerogels or films), for subsequent utilization as wound dressing materials. Growth curves using CNF (diameter <20nm) in suspension demonstrated an interesting dose-dependent inhibition of bacterial growth. In addition, analysis of biofilm formation (Pseudomonas aeruginosa PAO1) on nanocellulose aerogels (20g/m) revealed significantly less biofilm biomass with decreasing aerogel porosity and surface roughness. Importantly, virulence factor production by P. aeruginosa in the presence of nanocellulose materials, quantified for the first time, was unaffected (p>0.05) over 24h. These data demonstrate the potential of nanocellulose materials in the development of novel dressings that may afford significant clinical potential.

Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications

“…The versatility of nanocellulose is further reflected in its affinity for structural modification and functionalization. The porosity and surface topography of NFC hydrogels may be modified to augment the adsorptive and bacterial anti-adhesion capabilities of the wound dressing (Jack et al, 2017). Two studies (Rees et al, 2015) (Chinga-Carrasco andSyverud, 2014) highlighted the potential benefits of carboxymethylated-periodate oxidized nanocellulose (C-Periodate NC) for 3D bioprinting and wound dressing applications.…”

Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung epithelial cells and monocyte-derived macrophages

“…Due to its likely biocompatibility, CNF have been investigated in regenerative medicine as scaffolds for tissue-engineered meniscus, blood vessels, ligaments or tendons (Jia et al 2013;Lin and Dufresne 2014;Mathew et al 2012Mathew et al , 2013. Other biomedical applications of CNF are on wound healing (Basu et al 2017;Hakkarainen et al 2016;Jack et al 2017;Sun et al 2017;Syverud et al 2011), stem cell decorated threads for surgical suturing (Mertaniemi et al 2016), haemodialysis membranes (Ferraz et al 2013), long-lasting sustained drug delivery systems (Kolakovic et al 2012) or 3D cell culture scaffolds (Bhattacharya et al 2012;Malinen et al 2014).…”

Application of 3d Printing in Electro Induced Drug Delivery Based on Wound Temperature

“…A study conducted by 14 expressed a wound dressing is used for healing by using series of micro porous silicon rubber membrane with different pore sizes. Study shows the wound healing is done by using pores to control the absorbency of the wound dressing with chemical composition CNF 15 .…”