The Interactions Between Aligned Poly(L-Lactic Acid) Nanofibers and SH-SY5Y Cells In Vitro

Abstract

Aligned nanofibers have been regarded as promising nanomaterials in facilitating nerve regeneration. Investigating the interactions between aligned nanofibers and neuronal cells will be critically important for the design and application of aligned nanofibers in nerve tissue engineering. In this study, we explored the effects of electrospun Poly(L-Lactic Acid) (PLLA) aligned nanofibers on SH-SY5Y cells (a type of human neuroblastoma cell line) and specifically focused on the role of integrin in the PLLA aligned nanofiber-SH-SY5Y cell interaction. We found that PLLA aligned nanofibers could significantly guide the neurite outgrowth of SH-SY5Y cell, and promote the viability, proliferation, glucose and lactic acid metabolism of SH-SY5Y cell. This promotion effect could be alleviated when the functions of integrins on the SH-SY5Y cell membrane were hampered by pentapeptide GRGDS. Moreover, we found that PLLA aligned nanofibers could enhance the expression of phosphorylated-ERK1/2 (p-ERK1/2) in the SH-SY5Y cells and blocking the integrins would decrease p-ERK1/2 expression. These results suggested that PLLA aligned nanofibers might affect many cellular behaviors of SH-SY5Y cells via integrin mediated ERK pathway. Our findings provided more insights for understanding the interaction between aligned nanofibers and neuronal cells.

Electroactive Smart Materials for Neural Tissue Regeneration

“…In fact, extensive progress on bio-fabrication technologies has brought to light techniques such as electrospinning to create contact-guidance fibrous matrices which have been successfully applied for the growth of neural networks. One such example are PLLA nanofibrous scaffolds, which have been shown to promote the differentiation of PC12 cells 90, guide the neurite outgrowth of SH-SY5Y cells 91, guide neurite outgrowth of DRGs and Schwann cell migration along the aligned fibers 92,93 and promote cell adhesion, growth, proliferation and directed-neurite outgrowth of mouse induced pluripotent stem cells (iPSCs)-derived NSCs (iNSCs) 94. Furthermore, in order to improve biocompatibility, processability and biological effects, the surface modification of nanofibrous scaffolds has been considered. Aligned PLLA nanofibrous scaffolds coated with GO have been reported to promote rat Schwann cells proliferation, as well as proliferation, differentiation and NGF-dependent neurite growth of PC12 cells 95 .…”

NMR metabolomics to study the metabolic response of human osteoblasts to non‐poled and poled poly (L‐lactic) acid

“…Regarding PLLA, different polymeric morphologies (namely, films and aligned nanofibers) have been observed to trigger changes in glucose and lactic acid metabolisms in neuroblastoma cells . Such work has shown that cells grown on PLLA films use less glucose and produce more lactic acid, compared with those grown on nanofibers.…”

“…Regarding PLLA, different polymeric morphologies (namely, films and aligned nanofibers) have been observed to trigger changes in glucose and lactic acid metabolisms in neuroblastoma cells. [21] Such work has shown that cells grown on PLLA films use less glucose and produce more lactic acid, compared with those grown on nanofibers. Furthermore, PLLA electrospun nanofibers were shown to induce proteome adaptations in human neuroblastoma cells, in order to block cell proliferation and facilitate cell remodeling and adaption to biomaterial surface without causing immunological rejection.…”