The interactions of 9,10-phenanthrenequinone with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a potential site for toxic actions

Inhibitory effects of diesel exhaust components and flavonoids on 20α-hydroxysteroid dehydrogenase activity in mouse tissues

“…It has been reported that 9,10-PQ and 1,2-NQ serve as inhibitors of enzymes such as nitric oxide synthase [26], carbonyl reductase [27,28] and glyceraldehyde-3-phosphate dehydrogenase [29]. In the present study, 9,10-PQ was demonstrated to be a potent inhibitor for 20a-HSD activity in the cytosolic fractions from the liver and lung of male mice.…”

Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR

“…As an oxidant, a quinone molecule can produce reactive oxygen species (ROS) by reducing itself. Because the oneelectron reduction potential of 1,4-benzoquinone is far beyond the physiological relevant range, 1,4-benzoquinone is commonly regarded as an electrophile (35), which may form Michaeladducts with cysteine residues in QsrR as a way to tune the function of QsrR.…”

Profiling the NIH Small Molecule Repository for Compounds That Generate H2O2by Redox Cycling in Reducing Environments

“…[1][2][3][4]6,7 Several important classes of protein targets are susceptible to H 2 O 2 -mediated inactivation, including protein tyrosine phosphatases (PTPs), cysteine proteases (cathepsins and caspases), and metalloenzymes. [1][2][3][4][6][7][8][9][10][11][12][13][14] Redox cycling compounds (RCCs) generate H 2 O 2 in the presence of strong reducing agents such as DTT and tris(2-carboxyethyl)phosphine (TCEP), but not in the presence of weaker reducing agents like β-mercaptoethanol (BME), glutathione (GSH), or cysteine (Cys). 1,3,7 However, DTT and TCEP are commonly utilized in high-throughput screening (HTS) buffers to preserve the reduced state of critical amino acids and to maintain the catalytic activity or the folding of target proteins.…”