The interactions of piperonyl butoxide and analogues with the metabolic enzymes FE4 and CYP6CY3 of the green peach aphidMyzus persicae(Hemiptera: Aphididae)

Abstract

In vitro assays identified potential candidate synergists with high inhibitory potency. The in vivo confirmation of such results will allow consideration for a possible use in agriculture. © 2016 Society of Chemical Industry.

Biochemical evaluation of interactions between synergistic molecules and phase I enzymes involved in insecticide resistance in B‐ and Q‐type Bemisia tabaci (Hemiptera: Aleyrodidae)

“…Similar to the results found both with the M. persicae P450 (CYP6CY3), and with house fly P450 (CYP6D1): removal of the alkyl chain from the PBO‐like structures, resulted in a loss of inhibitory potency …”

“…The work described here explores the interactions between detoxifying enzymes of the whitefly B. tabaci and PBO analogues designed against the metabolic enzymes of another important agricultural pest, the green peach aphid Myzus persicae …”

“…The work described here explores the interactions between detoxifying enzymes of the whitefly B. tabaci and PBO analogues designed against the metabolic enzymes of another important agricultural pest, the green peach aphid Myzus persicae. 33 Previous findings demonstrated that PBO-pyrethroid applications may enhance the insecticide efficacy in resistant populations of B-type B. tabaci, following the inhibition of pyrethroid resistance-associated esterases. 41 The effect is more evident if PBO is applied before the insecticide, allowing a reduction of the insecticidal dose to be applied, as demonstrated with small-scale field trials performed by the same authors.…”

“…Derivatives 13 , 14 , 15 , 16 , 17 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 and 39 were synthesised following the synthesis procedures reported in Philippou et al Derivative 9 was synthesised according to Moores et al Derivatives 8 and 13 were synthesised according to Arvai et al Benzodioxole and benzodioxane derivatives, 2 , 3 , 4 , 5 , 6 and 44 , were synthesised following the synthesis procedure reported in Ugolini et al Benzodioxole derivative 45 was synthesised according to Tozzi . Dimethoxybenzene derivative was synthesised following the procedure reported in Panini et al …”

“…Derivatives 13, 14, 15, 16, 17, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 and 39 were synthesised following the synthesis procedures reported in Philippou et al 28 Derivative 9 was synthesised according to Moores et al 29 Derivatives 8 and 13 were synthesised according to Arvai et al 30 Benzodioxole and benzodioxane derivatives, 2, 3, 4, 5, 6 and 44, were synthesised following the synthesis procedure reported in Ugolini et al 31 Benzodioxole derivative 45 was synthesised according to Tozzi. 32 Dimethoxybenzene derivative was synthesised following the procedure reported in Panini et al 33 All the derivatives were purified by using the suitable purification techniques such as distillation, crystallisation and column chromatography. The physico-chemical characteristics and NMR analyses of the novel compounds are reported in the supporting information.…”