The Interactive Effect of These Bacterial Substrates on the Growth of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis

Abstract

In order to explore an effect of interaction of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis that are bacteria relevant to periodontal disease on its growth, the bacteria were incubated in trypticase soy hemin menadione broth at 37 o C CO2 incubator for 7 days through anaerobic jar by single and co-culture with heat treated dead bacteria under anaerobic gas pack. In order to confirm growth level, absorbance was measured and for confirming colony structure and form, it was observed with scanning electron microscope. In order to confirm an effect on pathogenicity of P. gingivalis, real time reverse transcriptase polymerase chain reaction was implemented for expression analysis for rgpA gene that produces HRgpA which is gingipain. As a result, the following conclusion was obtained. Colony formation of S. gordonii and P. gingivalis was increased by other dead bacteria and in case of F. nucleatum, its colony formation was showed an aspect of being increased by dead bacterium of P. gingivalis but decreased by dead bacterium of S. gordonii. Therefore, it is considered that the strains being used for this study would affect interactively through bacterial cell itself as well as their interaction factor at the time of colony formation.

Effects of Gossypetin from Hibiscus sabdariffa on Interleukin-6 Production in Porphyromonas gingivalis Lipopolysaccharide-Stimulated Human Gingival Fibroblasts

“…Periodontal disease is a chronic inflammatory and infectious disease caused by the complex toxicity of various oral microorganisms 1) . P. gingivalis, Aggregatibacter actinomycetemcomitans, and Tanerella forsythia, which are known to cause periodontitis, are mostly anaerobic Gramnegative bacteria, and periodontal disease-related various microorganisms, including these bacteria, interact and affect each other's growth and activity 3,16) . In particular, LPS in the outer membrane of Gram-negative bacteria acts as an important endotoxin factor that stimulates the host's immune system and causes inflammation 17) .…”

Oral Metagenomic Analysis Techniques

“…The virulence of the total microbiomes is increased by the core pathogen, Porphyromonas gingivalis, which interacts with secondary pathogens, like Streptococcus mitis (strain B6), and generally causes dysbiosis of the beneficial microbiota and inflammatory diseases [40][41][42][43][44] .…”