The interdependent transport of yeast vacuole Ca2+ and H+ and the role of phosphatidylinositol 3,5-bisphosphate

Zhang, Chi; Feng, Yilin; Balutowski, Adam; Miner, Gregory E.; Rivera‐Kohr, David A.; Hrabak, Michael R.; Sullivan, Katherine D.; Guo, Annie; Calderin, Jorge D.; Fratti, Rutilio A.

doi:10.1016/j.jbc.2022.102672

Cited by 5 publications

(4 citation statements)

References 91 publications

(144 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After Ca 2+ uptake, vacuoles release the cation upon the formation of trans -SNARE complexes between vesicles (Merz & Wickner, 2004). Our previous work used Cal520 fluorescence to show that Ca 2+ transport by isolated vacuoles was regulated by vacuole acidification and the lipid PI(3,5)P 2 (Miner et al , 2020; Zhang et al , 2022b). Here we found that both WT and elo3 Δ vacuole imported Ca 2+ upon the addition of ATP ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Others have found that regulating sphingolipid homeostasis is needed for full V-ATPase activity (Finnigan et al, 2011). Here we tested if isolated vacuoles could become acidified using an acridine orange fluorescence shift assay (Müller et al, 2002;Zhang et al, 2022bZhang et al, , 2022a. Acridine orange becomes trapped in acidic compartments due to its protonation.…”

Section: Vacuoles From Elo3d Cells Have Attenuated Fusion Activitymentioning

confidence: 99%

“…V-ATPase acidification of vacuoles was performed as previously described (Müller et al, 2003;Zhang et al, 2022aZhang et al, , 2022b. In vitro acidification reactions (60 µl) contained 20 µg BJ3505 background vacuoles, reaction buffer, ATP regenerating system, 10 µM CoA, 283 nM Pbi2, and 15 µM acridine orange (Sigma 65-61-2).…”

Section: Vacuole Acidificationmentioning

confidence: 99%

See 2 more Smart Citations

Sphingolipids regulate the tethering stage of vacuole fusion by affecting membrane fluidity

Hurst

Zhang

Kazmirchuk

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

ORCIDRutilio Fratti -https://orcid.org/0000-0001-9109-6666 Running title: Elo3p regulation of vacuole fusion via lipid rafts SummaryMany sphingolipids contain very-long chain fatty-acids with 26 carbons. The deletion of Elo3, the elongase that adds the final two carbons results in pleiotropic effects that negatively alter membrane fusion at the tethering and docking stages. AbstractThe role of sphingolipids in controlling the endolysosomal membrane trafficking remains unclear. Here, we show that in Saccharomyces cerevisiae sphingolipids containing very long-chain fatty-acids (VLCAs) promote homotypic vacuolar fusion. Yeast that lack the C26 VLCA elongase Elo3p display morphological and vacuolar abnormalities. Vacuoles isolated from these cells displayed reduced levels of in vitro fusion, which we traced to a block in tethering and docking. We found that C26 VLCFA deficient yeast mislocalize fusion markers, and the small GTPases Rho1p and Ypt7p fail to selectively concentrate at the boundary and vertex domains of vacuoles isolated from these yeasts. Surprisingly, we only observed mild changes to the localization of other regulatory lipids, but membrane fluidity and solubility was significantly altered. Taken together, these results suggest that sphingolipids containing C26 VLCFAs act as regulatory lipids in the homotypic vacuolar fusion cascade by assembling membrane microdomains that promote the protein and lipid machinery required for the tethering and docking of vacuoles.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Vacuoles From Elo3d Cells Have Attenuated Fusion Activitymentioning

confidence: 99%

See 1 more Smart Citation

Sphingolipids regulate the tethering stage of vacuole fusion by affecting membrane fluidity

Hurst

Zhang

Kazmirchuk

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In C. albicans , oxidative stress and ER stress are found to provoke vacuolar calcium release ( 87 , 88 ). The release of vacuolar calcium is related to the fusion and fission of the vacuolar membrane and occurs during the docking stage of membrane fusion ( 50 , 52 , 89 ). Besides, the vacuolar Ca 2+ /H + antiporter Vcx1 was previously found to elevate cytosolic calcium and block vacuole acidification important for many cellular processes ( 89 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

Chen,

Chang,

Lan

2023

Microbiol Spectr

View full text Add to dashboard Cite

Antimicrobial peptides play an important role in human innate defense against microbial pathogens. LL-37 is the only member of the human cathelicidin family of antimicrobial peptides. We previously showed that LL-37 targets the cell wall of Candida albicans , thereby reducing cell adhesion to plastic surfaces, oral epidermoid cells, and urinary bladders of mice. Moreover, LL-37 also induces cell wall and endoplasmic reticulum stress responses. Here, we found that LL-37 alters plasma membrane properties. In addition, LL-37 affected calcium homeostasis and induced the generation of reactive oxygen species associated with mitochondria and vacuoles. Finally, the Rim101 pathway was linked to the cellular response to LL-37. Together, these findings provide new insights into anti- C . albicans actions of LL-37 and highlight the complex cellular responses induced by LL-37. IMPORTANCE Candida albicans is a major human fungal pathogen, and antimicrobial peptides are key components of innate immunity. Studying the interplay between C. albicans and human antimicrobial peptides would enhance a better understanding of pathogen-host interactions. Moreover, potential applications of antimicrobial peptides in antifungal therapy have aroused great interest. This work explores new mechanisms of LL-37 against C. albicans and reveals the complex connection among calcium homeostasis, oxidative stress, signaling, and possibly organelle interaction. Notably, these findings support the possible use of antimicrobial peptides to prevent and treat fungal infections.

show abstract

Impact of Fab1/Vac14 inhibition on β-1,3-glucanase localization at the tip in Saccharomyces cerevisiae

Takeshita,

Iida

2024

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The interdependent transport of yeast vacuole Ca2+ and H+ and the role of phosphatidylinositol 3,5-bisphosphate

Cited by 5 publications

References 91 publications

Sphingolipids regulate the tethering stage of vacuole fusion by affecting membrane fluidity

Sphingolipids regulate the tethering stage of vacuole fusion by affecting membrane fluidity

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

Impact of Fab1/Vac14 inhibition on β-1,3-glucanase localization at the tip in Saccharomyces cerevisiae

Contact Info

Product

Resources

About