2013
DOI: 10.1371/journal.pone.0059578
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The Internal Architecture of Leukocyte Lipid Body Organelles Captured by Three-Dimensional Electron Microscopy Tomography

Abstract: Lipid bodies (LBs), also known as lipid droplets, are complex organelles of all eukaryotic cells linked to a variety of biological functions as well as to the development of human diseases. In cells from the immune system, such as eosinophils, neutrophils and macrophages, LBs are rapidly formed in the cytoplasm in response to inflammatory and infectious diseases and are sites of synthesis of eicosanoid lipid mediators. However, little is known about the structural organization of these organelles. It is unclea… Show more

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Cited by 29 publications
(48 citation statements)
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“…Live cell staining with ORO has a higher background and more structural variation than in PFA fixed cells, and the clear spherical architecture of the ORO-stained bodies is less apparent [13, 25, 53]. In the absence of fixation, these LB appear to present different surface tension characteristics, and their diversity in shape agrees more closely with our EM observations (HT and WEG, manuscript in submission) and those of other groups published recently [37]. A further consideration is that ORO does not stain all the LB in a mast cell, since we have recently shown that ORO + LB and those that stain positively with reagents such as filipin, lipidtox and nile red are not completely overlapping populations (WEG and HT, manuscript submitted), nevertheless, live cell ORO staining does differentiate a clear LB population and provides a counterpoint for the analysis of calcium signalling in and around these structures.…”
Section: Discussionsupporting
confidence: 90%
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“…Live cell staining with ORO has a higher background and more structural variation than in PFA fixed cells, and the clear spherical architecture of the ORO-stained bodies is less apparent [13, 25, 53]. In the absence of fixation, these LB appear to present different surface tension characteristics, and their diversity in shape agrees more closely with our EM observations (HT and WEG, manuscript in submission) and those of other groups published recently [37]. A further consideration is that ORO does not stain all the LB in a mast cell, since we have recently shown that ORO + LB and those that stain positively with reagents such as filipin, lipidtox and nile red are not completely overlapping populations (WEG and HT, manuscript submitted), nevertheless, live cell ORO staining does differentiate a clear LB population and provides a counterpoint for the analysis of calcium signalling in and around these structures.…”
Section: Discussionsupporting
confidence: 90%
“…Moreover, a study in ovarian follicles suggested that calcium phosphate deposits are localized within the core of lipid droplets [36]. A recent study suggests another intriguing possibility: Melo et al suggest that ER cisternae are present internally to eosinophil LB, reflecting the biogenesis of the LB from the ER itself [37]. If these ER cisternae retain calcium storage capacity, then they may contribute to the phenomena we observe.…”
Section: Discussionmentioning
confidence: 82%
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“…Automated electron tomography, a technique that offers 3D information at very high resolution, i. e., at transmission electron microscopy (TEM) level [32], applied to the study of human eosinophils, revealed that LDs from these cells contain in their cores an intricate system of membranes, organized as a network of tubules which resemble the ER [33]. Therefore, as indicated by other studies from our group [28, 34], leukocyte LDs are not homogeneous organelles but may exhibit membranous structures which explains the association of polar proteins within LD cores and may be important to understanding LD biogenesis [33]. …”
Section: Lds Composition and Structure In Leukocytesmentioning
confidence: 99%