The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding

Penezić, Ana; Aćimović, Jelena M.; Pavićević, Ivan D.; Jovanović, Vesna; Takić, Marija; Mandić, Ljuba M.

doi:10.1007/s00775-018-1628-7

Cited by 6 publications

(11 citation statements)

References 42 publications

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“…It was shown that fatty acids were able to modulate this effect. Finally, one of the latest investigations of this group revealed that the binding of copper cations Cu(II) with defatted HSA practically did not affect the reactivity of Cys34, while the addition of copper to the complex of HSA with FAs (oleic, myristic, or fish oil) increased the reactivity of the cysteine cumulatively [ 150 ].…”

Section: Interplay Of Binding Enzymatic and Antioxidant Propertiementioning

confidence: 99%

The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

et al. 2020

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As a carrier of many biologically active compounds, blood is exposed to oxidants to a greater extent than the intracellular environment. Serum albumin plays a key role in antioxidant defence under both normal and oxidative stress conditions. This review evaluates data published in the literature and from our own research on the mechanisms of the enzymatic and non-enzymatic activities of albumin that determine its participation in redox modulation of plasma and intercellular fluid. For the first time, the results of numerous clinical, biochemical, spectroscopic and computational experiments devoted to the study of allosteric modulation of the functional properties of the protein associated with its participation in antioxidant defence are analysed. It has been concluded that it is fundamentally possible to regulate the antioxidant properties of albumin with various ligands, and the binding and/or enzymatic features of the protein by changing its redox status. The perspectives for using the antioxidant properties of albumin in practice are discussed.

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Section: Interplay Of Binding Enzymatic and Antioxidant Propertiementioning

confidence: 99%

The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

et al. 2020

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“…Reduction of thiol groups and formation of disulfide bonds initiates albumin dimerization. A thiol group originating from Cys34 of human albumin is the major plasma antioxidant and important factor regulating the binding capacity for copper ions [41]. Formation of albumin dimers impairs binding and sequestration of free copper ions from the circulation.…”

Section: Discussionmentioning

confidence: 99%

Major trace elements and their binding proteins in the early phase of Covid-19 infection

et al. 2022

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Metal ions seem to play important roles in the pathogenesis of the novel coronavirus disease of 2019 and are under investigation as potential prognostic markers and supplements in therapeutic procedures. The present study was aimed at assessing the relationship between the most abundant essential microelements (iron, zinc and copper) and their major binding proteins in the circulation in the early stage of infection. The concentration of zinc ions was measured to be higher in infected than in healthy persons, as well as ratios zinc/albumin and zinc/alpha-2-macroglobulin. Increased zinc levels could be attributed to cellular redistribution of zinc ions or to a use of zinc supplementation (zinc concentration was above the upper reference limit in one-third of infected individuals). Immunoblot analysis of protein molecular forms revealed that infected persons had greater amounts of proteinase-bound alpha-2-macroglobulin tetramer and albumin monomer than healthy individuals. The quantities of these forms were correlated with the concentration of zinc ions (r = 0.42 and 0.55, respectively) in healthy persons, but correlations were lost in infected individuals, most likely due to very high zinc concentrations in some participants which were not proportionally followed by changes in the distribution of protein species. Although we still have to wait for a firm confirmation of the involvement of zinc in beneficial defense mechanisms in patients with Covid-19, it seems that this ion may contribute to the existence of circulating protein forms which are the most optimal.

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“…61 3. Albumin structure and binding sites for small molecules Albumin, the most abundant endogenous protein, 62 possesses the remarkable ability to interact with a wide range of molecules, including metal ions, 63 through both covalent and noncovalent interactions. 64 Covalent binding primarily occurs by utilizing the available nucleophilic groups in albumin, such as Cys34 and Lys residues.…”

Section: Molecular Engineering Of Cl-containing Cyanine Dye In the Ni...mentioning

confidence: 99%

Site-specific albumin tagging with chloride-containing near-infrared cyanine dyes: molecular engineering, mechanism, and imaging applications

Su,

Zhang,

Zhu

2023

Chem. Commun.

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The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding

Cited by 6 publications

References 42 publications

The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

Major trace elements and their binding proteins in the early phase of Covid-19 infection

Site-specific albumin tagging with chloride-containing near-infrared cyanine dyes: molecular engineering, mechanism, and imaging applications

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