The interplay between the vaginal microbiome and innate immunity in the focus of predictive, preventive, and personalized medical approach to combat HPV-induced cervical cancer

Kúdela, Erik; Líšková, Alena; Samec, Marek; Koklesová, Lenka; Holubeková, Veronika; Rokos, Tomas; Kozubik, Erik; Pribulová, Terézia; Zhai, Kevin; Büsselberg, Dietrich; Kubatka, Peter; Biringer, Kamil

doi:10.1007/s13167-021-00244-3

Cited by 26 publications

(12 citation statements)

References 197 publications

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“…Tumor-associated microbes could predict to the occurrence of cancer. On the other hand, microbes enriched in tumor pose unexpected effects on the therapeutic effect of different cancer treatments (including radiotherapy, chemotherapy, and especially immunotherapy) [23,24]. Our previous 16S rRNA sequencing analysis suggested that there were distinct microbial signatures in ovarian cancer tissue as compared to the normal fallopian tube tissue [4].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of the Microbiome of the Upper Reproductive Tract Focuses on Prediction, Prevention, and Personalized Medicine to Combat Ovarian Cancer

Zhou

Wang

et al. 2022

Preprint

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Purpose To explore whether there are particular genital microbiota compositions in the reproductive tract in ovarian cancer patients and analyze their potential impact on the preventive, precisive, and personalized treatment. Methods The samples of cotton swabs from the cervical, uterine cavity, fallopian tubes, and ovaries were collected in the operation of the malignant/benign lesion patients, and subjected to metagenomic shotgun sequencing analysis. The distribution characteristics of the microbiota in the reproductive tract of different patients was analyzed with the consideration of associated clinical data. Results Bacteria accounted for the highest proportion in each sample, with a median of 99%. The microbiota of the upper female reproductive tract are mainly from the cervical, charactering in low bacterial biomass and high bacterial diversity. Ovarian cancer had a distinct microbiota signature. The tubal ligation affects its microbial distribution. There were no different species on the surface of ovarian tissue between platinum-sensitive and platinum-resistant patients. Conclusion The identification of the distinct microbiota may benefit in the prediction as well as prevention of different types of ovarian cancer. Moreover, it may dedicate to strengthen the therapeutic effect of adjuvant treatment of cancer. These findings may provide new strategies for prevention, diagnosis, and therapeutic effects to enhance health outcomes for women with ovary cancer.

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Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of the Microbiome of the Upper Reproductive Tract Focuses on Prediction, Prevention, and Personalized Medicine to Combat Ovarian Cancer

Zhou

Wang

et al. 2022

Preprint

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“…Age, an intrinsic host factor, is associated with the risk of acquiring HPV infection. HPV is most prevalent among adolescents and young adults between 15 and 25 years of age, and then is increased by a lack of immune responses and squamous metaplasia because the basal cells are more susceptible to HPV infection associated with dysbiosis 27…”

Section: Discussionmentioning

confidence: 99%

Microbiota unbalance in relation to high-risk human papillomavirus cervical infection

Cherey

Payalef

Fleider

et al. 2023

International Journal of Gynecological Cancer

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ObjectivesTo assess vaginal dysfunction using basic vaginal states and the presence of lactobacillary microbiota in patients with human papillomavirus (HPV) infection with no squamous intra-epithelial lesions (SIL), with low-grade squamous intra-epithelial lesions (L-SIL), and with high-grade squamous intra-epithelial lesions (H-SIL) or squamous cell carcinoma compared with a control group (HPV-negative); to establish the prevalence of bacterial vaginosis, candidiasis, and trichomoniasis in the different age groups; and to characterize the species of lactobacilli according to the type of lesion.MethodsA cross-sectional study was carried out of patients who underwent clinical examination and collection of vaginal fornixes to study basic vaginal states and culture. Species identification of lactobacilli was performed by mass spectrometry. The results were analyzed using the χ2and Fisher’s tests; p<0.05 was considered significant. High-risk viral types were determined using a multiplex real-time polymerase chain reaction test.ResultsA total of 741 patients were analyzed and divided into three age groups: Group 1 aged 18–24 years (n=138), Group 2 aged 25–50 years (n=456), and Group 3 aged >50 years (n=147). All groups were further divided into an HPV-negative (control) group and an HPV-positive group without lesions, with L-SIL, or with H-SIL/squamous cell carcinoma. The prevalence of unbalanced basic vaginal states in patients with H-SIL/squamous cell carcinoma was 72.7% (p=0.03) in Group 1, 53.1% (p=0.05) in Group 2, and no cases of unbalance were detected in Group 3. The prevalence of bacterial vaginosis in women with H-SIL/squamous cell carcinoma in Group 1 was 54.5% and in Group 2 was 43.7%. Patients with H-SIL/squamous cell carcinoma had a prevalence of 21.4% ofLactobacillus crispatus, 42.9% ofL. jensenii, and 14.3% ofL. iners.ConclusionsA greater unbalance of vaginal microbiota was observed in patients with SIL, especially in those with H-SIL/squamous cell carcinoma. In this group, an increase inL. jenseniiandL. inerscompared with control was found.L. crispatushad a similar prevalence to the control group. It is important to characterize the lactobacilli species since the unbalance alters the vaginal microenvironment and acts as a co-factor in the persistence of HPV infection.

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“…There have been many studies on the correlation between local carcinogenesis and dysbiotic microbiota, pathobionts, and/or pathogens in the upper reproductive tract [ 11 , 26 – 28 ]. Importantly, microbes enriched in tumor pose unexpected effects on the therapeutic effect of different cancer treatments (including radiotherapy, chemotherapy, and especially immunotherapy) [ 29 , 30 ]. Our previous 16S rRNA sequencing analysis suggested that there were distinct microbial signatures in ovarian cancer tissue as compared to the normal fallopian tube tissue [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis of the microbiome of the upper reproductive tract: combating ovarian cancer through predictive, preventive, and personalized medicine

Zhou

Wang

et al. 2022

EPMA Journal

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Purpose We investigated whether ovarian cancer could alter the genital microbiota in a specific way with clinical values. Furthermore, we proposed how such changes could be envisioned in a paradigm of predictive, preventive, and personalized medicine (PPPM). Methods The samples were collected using cotton swabs from the cervical, uterine cavity, fallopian tubes, and ovaries of patients subjected to the surgical procedures for the malignant/benign lesions. All samples were then analyzed by metagenomic shotgun sequencing. The distribution patterns and characteristics of the microbiota in the reproductive tract of subjects were analyzed and were interpreted in relation to the clinical outcomes of the subjects. Results While the ovarian cancer was able to alter the genital microbiota, the bacteria were the dominant microorganisms in all samples across all cohorts in the study (median 99%). The microbiota of the upper female reproductive tract were mainly from the cervical, identified by low bacterial biomass and high bacterial diversity. Ovarian cancer had a distinct microbiota signature. The tubal ligation affects its microbial distribution. There were no different species on the surface of platinum-sensitive ovarian tissues compared to samples from platinum-resistant patients. Conclusion The ovarian cancer–induced changes in microbiota magnify the potential of microbiota as a biotherapeutic modality in the treatment of ovarian cancer in this study and very likely for several malignancies and other conditions. Our findings demonstrated, for the first time, that microbiota could be dissected and applied in more specific fashion based on a predictive, preventive, and personalized medicine (PPPM) model in the treatment of ovarian cancer. Utilizing microbiota portfolio in a PPPM system in ovarian cancer would provide a unique opportunity to a clinically intelligent and novel approach in the treatment of ovarian cancer as well as several other conditions and malignancies.

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The interplay between the vaginal microbiome and innate immunity in the focus of predictive, preventive, and personalized medical approach to combat HPV-induced cervical cancer

Cited by 26 publications

References 197 publications

Metagenomic Analysis of the Microbiome of the Upper Reproductive Tract Focuses on Prediction, Prevention, and Personalized Medicine to Combat Ovarian Cancer

Metagenomic Analysis of the Microbiome of the Upper Reproductive Tract Focuses on Prediction, Prevention, and Personalized Medicine to Combat Ovarian Cancer

Microbiota unbalance in relation to high-risk human papillomavirus cervical infection

Metagenomic analysis of the microbiome of the upper reproductive tract: combating ovarian cancer through predictive, preventive, and personalized medicine

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