The interplay of calponin, wnt signaling, and cytoskeleton protein governs transgenerational phenotypic abnormalities in drosophila exposed to zinc oxide nanoparticles

Anand, Avnika Singh; Verma, Kalyani; Amitabh,; Prasad, Dipti; Kohli, Ekta

doi:10.1016/j.cbi.2022.110284

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…In the highest concentrations evaluated (2.0 and 4.0 mg/mL), we observed low daily pupal formation (Figure ), high larval mortality rate, and low pupal formation (Figure ) for the exposure of animals to all NCs. It is already established that ZnO nanoparticles (NPs) are toxic to Drosophila and can induce the increased production of reactive oxygen species (ROS), resulting in oxidative stress and genotoxicity. − The high generation of ROS may be the cause of both the delay in postembryonic development and the high larval lethality after exposure to ZnO and ZnO:0.5Au [51,53,54]. ZnO NPs cause toxicity because they induce the generation of excess ROS and disrupt the flow of Ca 2+ , leading to cellular distress, which consequently induces a series of cellular damage, such as changes in Nrf2 signaling pathways, reduction of antioxidant proteins such as glutathione (GSH), redox imbalance, and mitochondrial dysfunction, which in the end lead to cell death. , …”

Section: Antimicrobial Activity Assaysmentioning

confidence: 99%

Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals

Oliveira,

Silva,

Floresta

et al. 2024

ACS Omega

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Doping nanoparticles represents a strategy for modulating the energy levels and surface states of nanocrystals (NCs), thereby enhancing their efficiency and mitigating toxicity. Thus, we herein focus on the successful synthesis of pure and gold (Au)-doped zinc oxide (ZnO) nanocrystals (NCs), investigating their physical−chemical properties and evaluating their applicability and toxicity through in vitro and in vivo assessments. The optical, structural, and photocatalytic characteristics of these NCs were scrutinized by using optical absorption (OA), X-ray diffraction (XRD), and methylene blue degradation, respectively. The formation and doping of the NCs were corroborated by the XRD and OA results. While the introduction of Au as a dopant did induce changes in the phase and size of ZnO, a high concentration of Au ions in ZnO led to a reduction in their photocatalytic activity. This demonstrated a restricted antibacterial efficacy against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Remarkably, Au-doped counterparts exhibited enhanced biocompatibility in comparison to ZnO, as evidenced in both in vitro (murine macrophage cells) and in vivo (Drosophila melanogaster) studies. Furthermore, confocal microscopy images showed a high luminescence of Au-doped ZnO NCs in vivo. Thus, this study underscores the potential of Au doping of ZnO NCs as a promising technique to enhance material properties and increase biocompatibility.

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Section: Antimicrobial Activity Assaysmentioning

confidence: 99%

Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals

Oliveira,

Silva,

Floresta

et al. 2024

ACS Omega

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Reproductive Toxicity of Nanomaterials Using Silver Nanoparticles and Drosophila as Models

Alaraby,

Abass,

Gutiérrez

et al. 2024

Molecules

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Reproductive toxicity is of special concern among the harmful effects induced by environmental pollutants; consequently, further studies on such a topic are required. To avoid the use of mammalians, lower eukaryotes like Drosophila are viable alternatives. This study addresses the gap in understanding the link between reproductive adverse outcomes and the presence of pollutants in reproductive organs by using Drosophila. Silver nanoparticles (AgNPs) were selected for their ease of internalization, detection, and widespread environmental presence. Both male and female flies were exposed to AgNPs (28 ± 4 nm, 100 and 400 µg/mL) for one week. Internalization and bioaccumulation of AgNPs in organs were assessed using transmission electron microscopy, confocal microscopy, and inductively coupled plasma mass spectrometry. Substantial accumulation of AgNPs in the gastrointestinal tract, Malpighian tubules, hemolymph, reproductive organs (ovaries and testes), and gametes were observed. The highest AgNP content was observed in testes. Exposure to AgNPs reduced ovary size and fecundity, though fertility and gender ratios of the offspring were unaffected. Significant deregulation of reproductive-related genes was observed, particularly in males. These findings underscore the utility of Drosophila as a model for evaluating reproductive hazards posed by AgNP exposure. The ease of AgNP internalization in Drosophila reproductive targets could be extrapolated to mammalians, raising concerns about the potential impacts of nanoparticle exposure on reproduction toxicity in humans.

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The interplay of calponin, wnt signaling, and cytoskeleton protein governs transgenerational phenotypic abnormalities in drosophila exposed to zinc oxide nanoparticles

Cited by 2 publications

References 41 publications

Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals

Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals

Reproductive Toxicity of Nanomaterials Using Silver Nanoparticles and Drosophila as Models

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