The Intertwining of Autophagy and the Ubiquitin Proteasome System in Podocyte (Patho)Physiology

Heintz, Lukas; Meyer-Schwesinger, Catherine

doi:10.33594/000000432

Cited by 15 publications

(7 citation statements)

References 188 publications

(248 reference statements)

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“…Matrix metalloproteinases (MMPs) are a kind of proteolytic enzymes, whose main function is to degrade ECM. MMP-9 is a member of the MMPs family, also known as gelatinase, which can degrade gelatin, type IV collagen and type V collagen in ECM, thus destroying the structure of ECM, inducing EMT, and promoting tumor cell invasion and metastasis ( Heintz and Meyer-Schwesinger, 2021 ). Other studies ( Lazaar and Panettieri, 2005 ; Khodabakhshi et al, 2021 ) have shown that interleukin-1 β (IL-1 β) plays an important role in tumor growth, proliferation, invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Relationship between serum TGF- β 1, MMP-9 and IL-1β and pathological features and prognosis in breast cancer

et al. 2023

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To investigate the levels of serum transforming growth factor-β 1 (TGF-β1), Matrix metalloproteinase-9 (MMP-9) and Interleukin-1 β (IL-1 β) in breast cancer (BC), and analyzing their relationship with pathological features and prognosis. Retrospective analysis of 86 subjects with BC (BC subgroup) and another 50 healthy subjects (control subgroup) during the same period were included. The clinical data were collected. In this research, in BC subgroup, The levels of serum TGF- β 1, MMP-9 and IL-1 β were significantly higher than those in control subgroup. The levels of TGF- β 1 and MMP-9 in serum of BC subjects was correlated with clinical stage, histological grade, lymph node metastasis and molecular classification, but not with age, tumor size and menopausal status. The level of serum IL-1 β was related to tumor size, clinical stage, histological grade and lymph node metastasis. Multivariate Logistic regression analysis showed that the high level of serum TGF- β1 and MMP-9 was independent risk factors for BC. High level of serum IL-1 β was not an independent risk factor for BC. The 3-year disease-free survival rate in high TGF- β1 subgroup and high MMP-9 subgroup was significantly lower than that in low TGF- β 1 subgroup and low MMP- 9 subgroup. To conclude, serum TGF- β 1, MMP-9 and IL-1β are highly expressed in BC, and the subjects with elevated serum levels of TGF- β 1 and MMP-9 suggests poor prognosis.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Relationship between serum TGF- β 1, MMP-9 and IL-1β and pathological features and prognosis in breast cancer

et al. 2023

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“…Much evidence suggests that dysregulation of the ubiquitin/proteasome system is strongly associated with autophagy [ 19 , 20 ]. In our study, LC3 immunofluorescence staining was positive (Fig.…”

Section: Resultsmentioning

confidence: 99%

EOAI, a ubiquitin-specific peptidase 5 inhibitor, prevents non-small cell lung cancer progression by inducing DNA damage

et al. 2023

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Objective Targeting deubiquitinases (DUBs) has emerged as a promising avenue for anticancer drug development. However, the effect and mechanism of pan-DUB inhibitor EOAI on non-small cell lung cancer (NSCLC) remains to be studied. Materials and methods The expression of ubiquitin-specific peptidase 5 (USP5) in NSCLC was evaluated by immunohistochemistry. The effect of the USP5 inhibitor, EOAI, on NSCLC cell growth and cell cycle was evaluated by CCK-8 and PI staining. Apoptosis was detected by Annexin V-FITC/PI double staining. Autophagy was examined by LC3 immunofluorescence. Comet assay and γ-H2AX immunofluorescence staining were used to detect DNA damage, and Western blotting was used to detect the expression of apoptosis, cycle, autophagy and DNA damage-related proteins. In vivo experiments demonstrated the effect of EOAI on NSCLC. Results We also found that USP5 was significantly upregulated in NSCLC tissues in this study. In addition, we show that EOAI can cause DNA damage in NSCLC cells while modulating the transcriptional activity of P53, thereby inducing cell cycle arrest in NSCLC cells, autophagy and apoptosis. In vivo experiments have shown that EOAI can inhibit tumors and synergistically enhance the anti-tumor effect of cisplatin. Conclusion USP5-mediated epigenetic regulation of oncogenes promotes the occurrence of NSCLC, which provides ideas for developing potential targeted therapy.

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“…In this analysis, phagosomes were found to be involved. Research has indicated a close relationship between phagosomes, DN, and autophagy [39,40]. Additionally, specific lncRNAs, such as AK044604 (Risa), have been found to inhibit autophagy, exacerbating podocyte injury in DN [41].…”

Section: Discussionmentioning

confidence: 99%

A novel lncRNA TCONS_00071187 upregulated by activated GSK3β promotes high glucose-induced mesangial cell proliferation in the diabetic nephropathy

Zhaoshou Yang,

Xiaoxiao Sun,

Xiaofeng Deng

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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Inhibiting mesangial cell proliferation is one of the strategies to control the early progression of diabetic nephropathy (DN). GSK3β is closely related to cell apoptosis as well as the development of DN, but whether it acts on the proliferation of mesangial cells is unclear. This study aimed to elucidate the role and mechanism of GSK3β-mediated lncRNA in high glucose-induced mesangial cell proliferation. HBZY-1 cells were used to establish the cell model of DN. The automatic cell counter was applied to assess cell proliferation. Flow cytometry was used to detect cell apoptosis and intracellular ROS levels. High-throughput transcriptomics sequencing was performed to detect the different expressions of long noncoding RNAs (lncRNAs) in the cell model of DN after knocking down the expression of GSK3β by the transfection of siRNA. The expression of RNA was detected by real-time PCR. In the cell model of DN using HBZY-1 cells, cell proliferation was enhanced accompanied by GSK3β activation and elevated apoptosis rate and reactive oxygen species (ROS) levels. A panel of novel lncRNAs, which were differentially expressed after GSK3β knockdown in the cell model of DN, were identified by high-throughput transcriptomics sequencing. Among them, the expression of TCONS_00071187 was upregulated under high glucose conditions while the knockdown of the GSK3β expression led to the downregulation of TCONS_00071187. The knockdown of TCONS_00071187 resulted in reduced mesangial cell proliferation, and decreased apoptosis rates and ROS levels. In conclusion, GSK3β promoted mesangial cell proliferation by upregulating TCONS_00071187, which led to enhanced ROS production under high glucose conditions in the cell model of DN. This study revealed the role of GSK3β medicated lncRNAs in the development of DN.

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The Intertwining of Autophagy and the Ubiquitin Proteasome System in Podocyte (Patho)Physiology

Cited by 15 publications

References 188 publications

RETRACTED: Relationship between serum TGF- β 1, MMP-9 and IL-1β and pathological features and prognosis in breast cancer

RETRACTED: Relationship between serum TGF- β 1, MMP-9 and IL-1β and pathological features and prognosis in breast cancer

EOAI, a ubiquitin-specific peptidase 5 inhibitor, prevents non-small cell lung cancer progression by inducing DNA damage

A novel lncRNA TCONS_00071187 upregulated by activated GSK3β promotes high glucose-induced mesangial cell proliferation in the diabetic nephropathy

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