The Intestinal Bacterial Community and Functional Potential of Litopenaeus vannamei in the Coastal Areas of China

Cheng, Yimeng; Ge, Chaorong; Li, Wei; Yao, Huaiying

doi:10.3390/microorganisms9091793

Cited by 15 publications

(3 citation statements)

References 84 publications

(132 reference statements)

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“…The analysis of species' relative abundance in the disinfectant groups and the control group showed that, at the phylum level, the dominant microflora were Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Cyanobacteria, among which Proteobacteria had the largest relative abundance, which was similar to the results of previous studies (Fan et al, 2019;Cheng et al, 2021). At the genus level, the dominant bacterial genera in the intestinal bacterial community of L. vannamei were Photobacterium, Vibrio, and Ruegeria.…”

Section: Discussionsupporting

confidence: 86%

Germicidal effect of hydrogen peroxide nano-silver ion composite disinfectant and its effect on the microbial community of shrimp intestine and rearing water

et al. 2023

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In this study, the inhibitory effect of different doses of hydrogen peroxide nano-silver ion composite disinfectant (HPS) on DIV1 and bacteria was analyzed, and the antibacterial activity of three disinfectants, HPS, potassium monopersulfate (KMPS) and calcium hypochlorite (Ca(ClO)2), was compared. In addition, 16S rDNA amplification sequencing technology was used to analyze the effects of these three disinfectants on the intestinal microflora of Litopenaeus vannamei and the structure and composition of water microflora. The results showed that HPS did not positively affect the survival rate of prawns infected with DIV1, which needs to be verified in future studies. In the experimental design range, the higher the dosage of HPS, the more obvious the killing effect on the number of Vibrio and total bacteria in rearing water, and the two showed a negative correlation. 5,104 OTUs were obtained based on 16S rDNA high-throughput sequencing, of which 3,012 (59.01%) and 1,475 (28.90%) OUTs were annotated at the phylum and genus levels, respectively. Proteobacteria, Firmicutes, Actinobacteriota, Bacteroidetes, and Cyanobacteria dominated the water samples at the phylum level. At the genus level, the dominant bacterial genera in the intestinal bacterial community of shrimp were Photobacterium, Vibrio, and Ruegeria. The most dominant bacteria genera in water samples were Vibrio, Ruegeria, Pseudoalteromonas, and Nautella. In the water samples, the composition and structure of the Ca(ClO)2 microbial community were relatively simple, and the species richness and diversity of Ca(ClO)2 were significantly lower than those of HPS and KMPS disinfectant groups at 12 h and 24 h (p < 0.05). In terms of inhibiting the diversity and richness of the microbial community, Ca(ClO)2 had a significant effect (p < 0.05), but the intestinal microbial community diversity of shrimp treated with HPS was higher than that of the other two groups. It is worth noting that, compared with the three disinfectants, HPS has the strongest killing effect on Vibrio and total bacteria, and has a certain positive significance for maintaining the stable state of the microbial community. This study provides a scientific basis for applying HPS in aquaculture and broadens the application range of HPS.

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Section: Discussionsupporting

confidence: 86%

Germicidal effect of hydrogen peroxide nano-silver ion composite disinfectant and its effect on the microbial community of shrimp intestine and rearing water

et al. 2023

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“…At the genus level (Figure 3c), the bacterial species initially found in shrimp samples were Ralstonia, Vibrio and Aliivibrio. These genera are known to originate from the intestinal tract of Penaeus vannamei, according to previous studies [48]. In group B, the dominant bacteria were Ralstonia, Alivibrio, Photobacterium, Moritella and Pseudoalteromonas.…”

Section: Species Annotation and Composition Analysismentioning

confidence: 82%

Growth Reduction of Vibrionaceae and Microflora Diversity in Ice-Stored Pacific White Shrimp (Penaeus vannamei) Treated with a Low-Frequency Electric Field

Xu,

Chen,

Liang

et al. 2024

Foods

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A novel storage technique that combines the low-frequency electric field (LFEF) and ice temperature was used to extend the shelf life of Pacific white shrimp (Penaeus vannamei). The study investigated the effect of LFEF treatment on the quality and microbial composition of Penaeus vannamei during storage at ice temperature. The results showed that the LFEF treatment significantly extended the shelf life of shrimp during storage at ice temperature. The total volatile base nitrogen (TVB-N) and pH of samples increased over time, while the total viable count (TVC) showed a trend of first decreasing and then increasing. Obviously, shrimp samples treated with LFEF had a lower pH, TVB-N and TVC values than the untreated samples (p < 0.05) at the middle and late stages of storage. LFEF treatment increased the diversity and altered the composition of the microbial communities in Penaeus vannamei. Additionally, the treatment led to a decrease in the relative abundance of dominant spoilage bacteria, including Aliivibrio, Photobacterium and Moritella, in Penaeus vannamei stored at ice temperature for 11 days. Furthermore, correlation analysis indicated that TVB-N and pH had a significant and positive correlation with Pseudoalteromonas, suggesting that Pseudoalteromonas had a greater impact on shrimp quality. This study supports the practical application of accelerated low-frequency electric field-assisted shrimp preservation as an effective means of maintaining shrimp meat quality.

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“…Thus, studying the potential, resident, and beneficial microbes is crucial for improving host health. To date, several amplicon sequence-based studies on P. vannamei have characterized the microbial communities in different habitats [ 6 ], developmental stages [ 6 ], disease conditions [ 59 ], and diet [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

In silico prediction of potential indigenous microbial biomarkers in Penaeus vannamei identified through meta-analysis and genome-scale metabolic modelling

Devika

Katneni

Jangam

et al. 2023

Environmental Microbiome

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Background Understanding the microbiome is crucial as it contributes to the metabolic health of the host and, upon dysbiosis, may influence disease development. With the recent surge in high-throughput sequencing technology, the availability of microbial genomic data has increased dramatically. Amplicon sequence-based analyses majorly profile microbial abundance and determine taxonomic markers. Furthermore, the availability of genome sequences for various microbial organisms has prompted the integration of genome-scale metabolic modelling that provides insights into the metabolic interactions influencing host health. However, the analysis from a single study may not be consistent, necessitating a meta-analysis. Results We conducted a meta-analysis and integrated with constraint-based metabolic modelling approach, focusing on the microbiome of pacific white shrimp Penaeus vannamei, an extensively cultured marine candidate species. Meta-analysis revealed that Acinetobacter and Alteromonas are significant indicators of "health" and "disease" specific taxonomic biomarkers, respectively. Further, we enumerated metabolic interactions among the taxonomic biomarkers by applying a constraint-based approach to the community metabolic models (4416 pairs). Under different nutrient environments, a constraint-based flux simulation identified five beneficial species: Acinetobacter spWCHA55, Acinetobacter tandoii SE63, Bifidobacterium pseudolongum 49 D6, Brevundimonas pondensis LVF1, and Lutibacter profundi LP1 mediating parasitic interactions majorly under sucrose environment in the pairwise community. The study also reports the healthy biomarkers that can co-exist and have functionally dependent relationships to maintain a healthy state in the host. Conclusions Toward this, we collected and re-analysed the amplicon sequence data of P. vannamei (encompassing 117 healthy and 142 disease datasets). By capturing the taxonomic biomarkers and modelling the metabolic interaction between them, our study provides a valuable resource, a first-of-its-kind analysis in aquaculture scenario toward a sustainable shrimp farming.

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The Intestinal Bacterial Community and Functional Potential of Litopenaeus vannamei in the Coastal Areas of China

Cited by 15 publications

References 84 publications

Germicidal effect of hydrogen peroxide nano-silver ion composite disinfectant and its effect on the microbial community of shrimp intestine and rearing water

Germicidal effect of hydrogen peroxide nano-silver ion composite disinfectant and its effect on the microbial community of shrimp intestine and rearing water

Growth Reduction of Vibrionaceae and Microflora Diversity in Ice-Stored Pacific White Shrimp (Penaeus vannamei) Treated with a Low-Frequency Electric Field

In silico prediction of potential indigenous microbial biomarkers in Penaeus vannamei identified through meta-analysis and genome-scale metabolic modelling

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