The intracellular domain of duck plague virus glycoprotein E affects UL11 protein incorporation into viral particles

Yang, Linjiang; Shen, Bingjie; Wang, Mingshu; Cheng, Anchun; Yang, Qiao; Wu, Ying; Huang, Juan; Tian, Bin; Jia, Renyong; Liu, Mafeng; Zhu, Dekang; Chen, Shun; Zhao, Xinxin; Zhang, Shaqiu; Ou, Xumin; Mao, Sai; Gao, Qun; Sun, Di

doi:10.1016/j.vetmic.2021.109078

Cited by 11 publications

(11 citation statements)

References 38 publications

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“…With the above results, the reduced expression of the UL11 could affect the viral replication. The ICP22 ( Fraser and Rice, 2007 ; Li et al, 2020 ), ICP27 ( Tang et al, 2019 ), and ICP4 ( Frasson et al, 2021 ) are immediate early genes, and UL11 ( Yang et al, 2021 ), UL16 ( Oshima et al, 1998 ; He et al, 2012 ), UL21 ( Yang et al, 2020 ), gE ( Po’ka et al, 2017 ) and gB ( Ramachandran et al, 2010 ) are late genes, and the US3 ( Labiuk et al, 2010 ) is an early gene. Nevertheless, how DPV UL11 takes part in the viral replication process, we still do not know.…”

Section: Resultsmentioning

confidence: 99%

“…Also, the prohibition could precipitate the UL11 ( Figure 5B ). Previously we determined that UL11G2A is important for the Golgi-apparatus and membrane localization ( Yang et al, 2021 ). So the relationship between the G2A and lipid raft is worth exploring.…”

Section: Resultsmentioning

confidence: 99%

“…The pCAGGS-UL11-3 × Flag, pCAGGS-UL11G2A-3 × Flag, pEGFP-N1-UL11 plasmids were used in the homologous recombination method for cloning. pCAGGS-UL11G2A-3 × Flag (were site-directed mutated by point mutation kit (Transgen, CHN) ( Yang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The rabbit antibodies of gB , UL21 , gE, and mouse antibodies of Us3, UL16, ICP27, ICP22 were provided by our lab ( Yang et al, 2021 ). Anti-Flag monoclonal antibody was bought at the MBL with a dilution of 1:3,000 (MBL, JPN).…”

Section: Methodsmentioning

confidence: 99%

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UL11 Protein Is a Key Participant of the Duck Plague Virus in Its Life Cycle

et al. 2022

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Tegument protein UL11 plays a critical role in the life cycle of herpesviruses. The UL11 protein of herpesviruses is important for viral particle entry, release, assembly, and secondary envelopment. Lipid raft is cholesterol-rich functional microdomains in cell membranes, which plays an important role in signal transduction and substance transport. Flotillin and prohibition, which are considered to be specific markers of lipid raft. However, little is known about the function of duck plague virus (DPV) UL11 in the life cycle of the viruses and the relationship between the lipid raft and UL11. In this study, an interference plasmid shRNA126 for UL11 was used. Results showed that UL11 is involved in the replication, cell to cell spread, viral particle assembly, and release processes. Furthermore, UL11 was verified that it could interact with the lipid raft through sucrose density gradient centrifugation and that function correlates with the second glycine of the UL11. When the lipid raft was depleted using the methyl-β-cyclodextrin, the release of the DPV was decreased. Moreover, UL11 can decrease several relative viral genes mRNA levels by qRT-PCR and Western blot test. Altogether, these results highlight an important role for UL11 protein in the viral replication cycle.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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UL11 Protein Is a Key Participant of the Duck Plague Virus in Its Life Cycle

et al. 2022

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“…The expression of CCV genes during infection of CCO cells can be classified into three main phases, including immediate-early (IE), early, and late phases, similar to those of other herpesviruses [25]. The temporal expression of CCV ORF59 was characterized during CCV infection over a 24 h time course using qRT-PCR and Western blotting (Figure 1A).…”

Section: Orf59 Is a Late Expression Gene During CCV Infectionmentioning

confidence: 96%

Characterization of Ictalurid herpesvirus 1 Glycoprotein ORF59 and Its Potential Role on Virus Entry into the Host Cells

Chen

et al. 2021

Viruses

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The channel catfish virus (CCV, Ictalurid herpesvirus 1) has caused sustained economic losses in the fish industry because of its strong infectivity and pathogenicity. Thus, it is necessary to determine the function of viral proteins in the CCV infection process. The present study aimed to characterize CCV glycoprotein ORF59 and explore its impact on virus infection in host cells. Firstly, its exclusive presence in the membrane fraction of the cell lysate and subcellular localization verified that CCV ORF59 is a viral membrane protein expressed at late-stage infection. A protein blocking assay using purified His6 tagged ORF59, expressed in sf9 insect cells using a baculovirus expression system, indicated a dose-dependent inhibitory effect of recombinant ORF59 protein on virus invasion. Knockdown of the ORF59 using a short hairpin (shRNA) showed that ORF59 silencing decreased the production of infectious virus particles in channel catfish ovary cells. The results of this study suggest that recombinant ORF59 protein might inhibit CCV entry into the host cells. These findings will promote future studies of the key functions of glycoprotein ORF59 during CCV infection.

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