The intriguing role of collagen on the rheology of cancer cell spheroids

Tsvirkun, Daria; Révilloud, Jean; Giannetti, Arianna; Verdier, Claude

doi:10.1016/j.jbiomech.2022.111229

Cited by 9 publications

(16 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the Young's Modulus only gives a partial insight into the mechanical response of the material. For example, a recent work 51 highlighted how different amounts of collagen affect both tissue stiffness and viscosity in cellular aggregates. Future studies should focus on the characterization of layer-specific viscoelastic effects.…”

Section: Discussionmentioning

confidence: 99%

Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence

et al. 2023

View full text Add to dashboard Cite

Tissue mechanics determines tissue homeostasis, disease development and progression. Bladder strongly relies on its mechanical properties to perform its physiological function, but these are poorly unveiled under normal and pathological conditions. Here we characterize the mechanical fingerprints at the micro-scale level of the three tissue layers which compose the healthy bladder wall, and identify modifications associated with the onset and progression of pathological conditions (i.e., actinic cystitis and bladder cancer). We use two indentation-based instruments (an Atomic Force Microscope and a nanoindenter) and compare the micromechanical maps with a comprehensive histological analysis. We find that the healthy bladder wall is a mechanically inhomogeneous tissue, with a gradient of increasing stiffness from the urothelium to the lamina propria, which gradually decreases when reaching the muscle outer layer. Stiffening in fibrotic tissues correlate with increased deposition of dense extracellular matrix in the lamina propria. An increase in tissue compliance is observed before the onset and invasion of the tumor. By providing high resolution micromechanical investigation of each tissue layer of the bladder, we depict the intrinsic mechanical heterogeneity of the layers of a healthy bladder as compared with the mechanical properties alterations associated with either actinic cystitis or bladder tumor.

show abstract

Section: Discussionmentioning

confidence: 99%

Micro-mechanical fingerprints of the rat bladder change in actinic cystitis and tumor presence

et al. 2023

View full text Add to dashboard Cite

show abstract

“…This is carried out in detail by varying the model parameters. Finally we optimize these parameters to rationalize previous results [20] obtained for spheroids containing an excess of collagen.…”

mentioning

confidence: 76%

“…drained by water or the culture medium present in the spheroid [19]. But the influence of a larger collagen content may allow the building up of a new microstructure made of interconnected fibers responsible for the larger mechanical moduli observed [20]. It is definitely accepted that the whole spheroid exhibits a viscoelastic pattern, due to the presence of cells embedded within the ECM, a gel-like system.…”

mentioning

confidence: 99%

“…Spheroids were prepared in 15 μL hanging droplets containing 5000 T24 cells in culture medium including small amounts of collagen (initial concentration c 0 = 0.01 mg/mL or 0.03 mg/mL) as described previously [20]. This method allows cells to accumulate ECM without having too much collagen around the spheroids, which would render AFM measurements more difficult.…”

mentioning

confidence: 99%

“…Values of the prefactor are shown in table 1. This shows that even though the collagen modulus G * m (ω) is rather small (plateau of G around 200 Pa), as compared to cell's moduli (plateau of G at 500 Pa), the amplification due to spherical inclusions such as cells could lead to the high values obtained in spheroids [20]. The simplified formula In what follows, calculations are made by accounting for all terms in the set of eqs.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A rheological model for spheroids including extra-cellular matrix

Verdier,

Palade

2024

EPL

Self Cite

View full text Add to dashboard Cite

The rheology of spheroids has been studied intensively recently and it was shown that the presence of the extra-cellular matrix (ECM) can have significant effects on the overall behaviour of these biological systems. Collagen I can indeed be a proxy between cells and bring new intriguing effects, as its content increases. To investigate these effects further, a two-phase emulsion model is proposed including interactions between cells and the ECM. Starting with the single cell and collagen individual viscoelastic properties, the model can be tested against previously obtained data for spheroids. The model has interesting features and capabilities for it covers a variety of behaviours and uses fitting parameters such as collagen and cell concentration, as well as adhesion energy. It is shown that the final intercellular collagen content can be large as compared to the initial one, and that this increase in collagen content induces a larger packing of cells, together with a larger adhesion energy.

show abstract