The Introduction and Expression of Foreign Genes in Plants

Shah, Dilip M.; Tumer, Nilgun E.; Fischhoff, David A.; Horsch, Robert B.; Rogers, Stephen L.; Fraley, Robert T.; Jaworski, Ernest G.

doi:10.1080/02648725.1987.10647835

Cited by 5 publications

(2 citation statements)

References 102 publications

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“…Transformation mediated by A. tumefaciens has been used in many plant species because of its efficiency, simplicity, and stability (Joshi and Joshi 1991;Hansen et al 1997;Shah et al 1987;Yu et al 2007). Sweet potato transformation using A. tumefaciens has been attempted from various explant tissues (Newell et al 1995;Gama et al 1996;Morán et al 1998;Mitchell et al 1998;Otani et al 1998Otani et al , 2001Otani et al , 2003Cipriani et al 1999;Wakita et al 2001;Kimura et al 2001;Song et al 2004;Shimada et al 2006;Luo et al 2006), but most of these studies showed a genotype-dependent response along with low transformation and regeneration efficiency.…”

Section: Introductionmentioning

confidence: 99%

Transgenic sweet potato expressing mammalian cytochrome P450

Anwar

Watanabe

2010

Plant Cell Tiss Organ Cult

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Sweet potato [Ipomoea batatas (L.) Lam] is considered to be recalcitrant to transformation and regeneration because of its genotype-dependent in vitro responses. The lack of a genotype-independent transformation and regeneration system limits biotechnological applications in this plant species. To establish a transformation system for a diverse group of sweet potato genotypes, we examined sweet potato regeneration after transformation in five cultivars. An Agrobacterium tumefaciens transformation system was used for the introduction of mammalian cytochrome P450 genes, which are capable of conferring herbicide tolerance. Among the different factors studied, including explant type, plasmid vectors, and auxin type in the initiation media, auxin type had the greatest effect on the regeneration response. Of the auxins tested, only 4-fluorophenoxyacetic acid (4FA) induced regeneration from all cultivars. In terms of the quality of calli, 4FA promoted the induction of type I calli, which were capable of somatic embryo formation, whereas type II calli fail to produce somatic embryos. The frequency of somatic embryo formation was also affected by the composition of the embryo-induction media. Transgenic plants were regenerated from all cultivars. The stable integration and expression of transgenes was confirmed by several approaches. This Agrobacterium-mediated transformation system should be applicable to a wide range of sweet potato cultivars.

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Section: Introductionmentioning

confidence: 99%

Transgenic sweet potato expressing mammalian cytochrome P450

Anwar

Watanabe

2010

Plant Cell Tiss Organ Cult

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“…These are to be used in crossed breeding approaches to transfer the trait into additional, locally important varieties. A variety of methods to introduce foreign DNA into plant cells have been attempted over the past several years (Shah et al 2013). The broad range in carotene types and concentrations among Cucurbita species and sweet potato (Ipomoea batatas Lam.)…”

Section: Introductionmentioning

confidence: 99%

Stability of PAC (Psy-2A-CrtI) gene and agronomic traits in the F2:3 of IR36/PAC transgenic plants

Lee

Kim

2015

Journal of Integrative Agriculture

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This study was carried out to evaluate the expression of yellow endosperm color in the progeny of PAC (β-carotene gene) transgenic plants crossed with IR36. The selected 25 lines of F 2 progeny exhibited stable expression of yellow endosperm color and high agronomic characteristics. IR36 and PAC transgenic plant of the grain length, width and ratio showed the same tendency of normal distribution. For progeny selection, colorimeters were as employed to distinguish differing visible colors. Correlation analysis was conducted to investigate the relationship between b* value and the expression of specific genes. b* value was associated with the relative expression of the PAC-Rev-MAR product by real-time PCR and t-test revealed significant difference. Based on this study, correlation between colorimetric values and real-time PCR was effective to detect gene expression. Yellow endosperm progenies represent a new genetic pool that might be useful to increase the genetic diversity of yellow endosperm rice.

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Downstream Processing of Potato Proteins

Jervis

1989

Protein-Dye Interactions: Developments and Applications

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The Introduction and Expression of Foreign Genes in Plants

Cited by 5 publications

References 102 publications

Transgenic sweet potato expressing mammalian cytochrome P450

Transgenic sweet potato expressing mammalian cytochrome P450

Stability of PAC (Psy-2A-CrtI) gene and agronomic traits in the F2:3 of IR36/PAC transgenic plants

Downstream Processing of Potato Proteins

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